Detection of DDT and carbaryl pesticides in honey by means of immunosensors based on high fundamental frequency quartz crystal microbalance (HFF-QCM)

[EN] BACKGROUND: In recent years there has been a concern about the presence of pesticides in honey because residues of DDT and carbaryl were found in honey samples. Traditional techniques, such as chromatography, reach the required limits of detection (LOD) but are not suitable for in situ implemen...

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Autores: Cervera-Chiner, Lourdes|||0000-0002-6895-4957, Arnau Vives, Antonio|||0000-0002-5709-1690, Jiménez Jiménez, Yolanda|||0000-0003-4835-9007, March, Carmen, Montoya, Ángel
Formato: artículo
Fecha de publicación:2020
País:España
Recursos:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/149414
Acesso em linha:https://riunet.upv.es/handle/10251/149414
Access Level:acceso abierto
Palavra-chave:Immunosensors
HFF-QCM
Pesticides
Carbaryl
DDT
Honey
TECNOLOGIA DE ALIMENTOS
TECNOLOGIA ELECTRONICA
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repository_id_str
spelling Detection of DDT and carbaryl pesticides in honey by means of immunosensors based on high fundamental frequency quartz crystal microbalance (HFF-QCM)Cervera-Chiner, Lourdes|||0000-0002-6895-4957Arnau Vives, Antonio|||0000-0002-5709-1690Jiménez Jiménez, Yolanda|||0000-0003-4835-9007March, CarmenMontoya, ÁngelImmunosensorsHFF-QCMPesticidesCarbarylDDTHoneyTECNOLOGIA DE ALIMENTOSTECNOLOGIA ELECTRONICA[EN] BACKGROUND: In recent years there has been a concern about the presence of pesticides in honey because residues of DDT and carbaryl were found in honey samples. Traditional techniques, such as chromatography, reach the required limits of detection (LOD) but are not suitable for in situ implementation in the honey-packaging industry due to their high cost and the need for highly quali¿ed staff for routine operation. Biosensors offer simplicity, low cost, and easy handling for analytical purposes in food applications. RESULTS: Piezoelectric immunosensors based on high fundamental frequency quartz crystal microbalance (HFF-QCM) have been developed for the detection of carbaryl and DDT in honey. Biorecognition was based on competitive immunoassays in the conjugate-coated format, using monoclonal antibodies as speci¿c immunoreagents. The assay LODs attained by the HFF-QCM immunosensors were 0.05 ~gL¿1 for carbaryl and 0.24 ~gL¿1 for DDT, reaching a similar level of detectability to that of the usual reference techniques. The practical LODs in honey samples were 8 ~gkg¿1 for carbaryl and 24 ~gkg¿1 for DDT. The immunosensors¿ analytical performance allow the detection of these pesticides in honey at EU regulatory levels with good accuracy (recovery percentages ranging from 94% to 130% within the working range of each pesticide standard curve) and precision (coef¿cients of variation in the 9¿36% range). CONCLUSION: The proposed immunosensor is a promising analytical tool that could be implemented for quality control in the honey packaging industry, to simplify and to reduce the cost of the routine pesticide analysis in this appreciated natural food.This research was funded by the Spanish Ministry of Economy and Competitiveness with FEDER funds (AGL Project 2013-48646-R). L.C.C. was the recipient of a PhD fellowship from the European Social Fund and the Generalitat Valenciana (ACIF / 2016/132)John Wiley & SonsDepartamento de Tecnología de AlimentosDepartamento de Ingeniería ElectrónicaEscuela Técnica Superior de Ingeniería de TelecomunicaciónEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalCentro de Investigación e Innovación en BioingenieríaEuropean Social FundGeneralitat ValencianaMinisterio de Economía y CompetitividadRepositorio Institucional de la Universitat Politècnica de València Riunet20202020-01-21journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://riunet.upv.es/handle/10251/149414reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 AGL2013-48646-R DISEÑO DE UNA PLATAFORMA BASADA EN MATRICES DE SENSORES DE CUARZO DE ALTA RESOLUCION PARA HTS (HIGH-THROUGHPUT SCREENING) DE RESIDUOS EN MIELESGeneralitat Valenciana https://doi.org/10.13039/501100003359 ACIF%2F2016%2F132open accesshttp://purl.org/coar/access_right/c_abf2Reserva de todos los derechoshttp://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1494142026-06-13T07:49:27Z
dc.title.none.fl_str_mv Detection of DDT and carbaryl pesticides in honey by means of immunosensors based on high fundamental frequency quartz crystal microbalance (HFF-QCM)
title Detection of DDT and carbaryl pesticides in honey by means of immunosensors based on high fundamental frequency quartz crystal microbalance (HFF-QCM)
spellingShingle Detection of DDT and carbaryl pesticides in honey by means of immunosensors based on high fundamental frequency quartz crystal microbalance (HFF-QCM)
Cervera-Chiner, Lourdes|||0000-0002-6895-4957
Immunosensors
HFF-QCM
Pesticides
Carbaryl
DDT
Honey
TECNOLOGIA DE ALIMENTOS
TECNOLOGIA ELECTRONICA
title_short Detection of DDT and carbaryl pesticides in honey by means of immunosensors based on high fundamental frequency quartz crystal microbalance (HFF-QCM)
title_full Detection of DDT and carbaryl pesticides in honey by means of immunosensors based on high fundamental frequency quartz crystal microbalance (HFF-QCM)
title_fullStr Detection of DDT and carbaryl pesticides in honey by means of immunosensors based on high fundamental frequency quartz crystal microbalance (HFF-QCM)
title_full_unstemmed Detection of DDT and carbaryl pesticides in honey by means of immunosensors based on high fundamental frequency quartz crystal microbalance (HFF-QCM)
title_sort Detection of DDT and carbaryl pesticides in honey by means of immunosensors based on high fundamental frequency quartz crystal microbalance (HFF-QCM)
dc.creator.none.fl_str_mv Cervera-Chiner, Lourdes|||0000-0002-6895-4957
Arnau Vives, Antonio|||0000-0002-5709-1690
Jiménez Jiménez, Yolanda|||0000-0003-4835-9007
March, Carmen
Montoya, Ángel
author Cervera-Chiner, Lourdes|||0000-0002-6895-4957
author_facet Cervera-Chiner, Lourdes|||0000-0002-6895-4957
Arnau Vives, Antonio|||0000-0002-5709-1690
Jiménez Jiménez, Yolanda|||0000-0003-4835-9007
March, Carmen
Montoya, Ángel
author_role author
author2 Arnau Vives, Antonio|||0000-0002-5709-1690
Jiménez Jiménez, Yolanda|||0000-0003-4835-9007
March, Carmen
Montoya, Ángel
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Tecnología de Alimentos
Departamento de Ingeniería Electrónica
Escuela Técnica Superior de Ingeniería de Telecomunicación
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
Centro de Investigación e Innovación en Bioingeniería
European Social Fund
Generalitat Valenciana
Ministerio de Economía y Competitividad
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Immunosensors
HFF-QCM
Pesticides
Carbaryl
DDT
Honey
TECNOLOGIA DE ALIMENTOS
TECNOLOGIA ELECTRONICA
topic Immunosensors
HFF-QCM
Pesticides
Carbaryl
DDT
Honey
TECNOLOGIA DE ALIMENTOS
TECNOLOGIA ELECTRONICA
description [EN] BACKGROUND: In recent years there has been a concern about the presence of pesticides in honey because residues of DDT and carbaryl were found in honey samples. Traditional techniques, such as chromatography, reach the required limits of detection (LOD) but are not suitable for in situ implementation in the honey-packaging industry due to their high cost and the need for highly quali¿ed staff for routine operation. Biosensors offer simplicity, low cost, and easy handling for analytical purposes in food applications. RESULTS: Piezoelectric immunosensors based on high fundamental frequency quartz crystal microbalance (HFF-QCM) have been developed for the detection of carbaryl and DDT in honey. Biorecognition was based on competitive immunoassays in the conjugate-coated format, using monoclonal antibodies as speci¿c immunoreagents. The assay LODs attained by the HFF-QCM immunosensors were 0.05 ~gL¿1 for carbaryl and 0.24 ~gL¿1 for DDT, reaching a similar level of detectability to that of the usual reference techniques. The practical LODs in honey samples were 8 ~gkg¿1 for carbaryl and 24 ~gkg¿1 for DDT. The immunosensors¿ analytical performance allow the detection of these pesticides in honey at EU regulatory levels with good accuracy (recovery percentages ranging from 94% to 130% within the working range of each pesticide standard curve) and precision (coef¿cients of variation in the 9¿36% range). CONCLUSION: The proposed immunosensor is a promising analytical tool that could be implemented for quality control in the honey packaging industry, to simplify and to reduce the cost of the routine pesticide analysis in this appreciated natural food.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-21
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/149414
url https://riunet.upv.es/handle/10251/149414
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 AGL2013-48646-R DISEÑO DE UNA PLATAFORMA BASADA EN MATRICES DE SENSORES DE CUARZO DE ALTA RESOLUCION PARA HTS (HIGH-THROUGHPUT SCREENING) DE RESIDUOS EN MIELES
Generalitat Valenciana https://doi.org/10.13039/501100003359 ACIF%2F2016%2F132
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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