Detection of alien genetic introgressions in bread wheat using dot-blot genomic hybridisation

Simple, reliable methods for the identification of alien genetic introgressions are required in plant breeding programmes. The use of genomic dot-blot hybridisation allows the detection of small Hordeum chilense genomic introgressions in the descendants of genetic crosses between wheat and H. chilen...

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Detalles Bibliográficos
Autores: Rey-Santomé, María Dolores, Prieto, Pilar
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/157118
Acceso en línea:http://hdl.handle.net/10261/157118
Access Level:acceso abierto
Palabra clave:Dot-blot hybridisation
Genomic introgressions
H. chilense
Bread wheat
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spelling Detection of alien genetic introgressions in bread wheat using dot-blot genomic hybridisationRey-Santomé, María DoloresPrieto, PilarDot-blot hybridisationGenomic introgressionsH. chilenseBread wheatSimple, reliable methods for the identification of alien genetic introgressions are required in plant breeding programmes. The use of genomic dot-blot hybridisation allows the detection of small Hordeum chilense genomic introgressions in the descendants of genetic crosses between wheat and H. chilense addition or substitution lines in wheat when molecular markers are difficult to use. Based on genomic in situ hybridisation, DNA samples from wheat lines carrying putatively H. chilense introgressions were immobilised on a membrane, blocked with wheat genomic DNA and hybridised with biotin-labelled H. chilense genomic DNA as a probe. This dot-blot screening reduced the number of plants necessary to be analysed by molecular markers or in situ hybridisation, saving time and money. The technique was sensitive enough to detect a minimum of 5 ng of total genomic DNA immobilised on the membrane or about 1/420 dilution of H. chilense genomic DNA in the wheat background. The robustness of the technique was verified by in situ hybridisation. In addition, the detection of other wheat relative species such as Hordeum vulgare, Secale cereale and Agropyron cristatum in the wheat background was also reported.This research was supported by grants AGL2015-64833R from the Spanish Ministerio de Economía y Competitividad (MINECO) and ERC-StG-243118 from the FP7 and The European Regional Development Fund (FEDER) from the European Union.Peer reviewedSpringer NatureMinisterio de Economía y Competitividad (España)European Research CouncilEuropean CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201720172017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/157118reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-64833-Rinfo:eu-repo/grantAgreement/EC/FP7/243118http://doi.org/10.1007/s11032-017-0629-5http://creativecommons.org/licenses/by/4.0/Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1571182026-05-22T06:33:51Z
dc.title.none.fl_str_mv Detection of alien genetic introgressions in bread wheat using dot-blot genomic hybridisation
title Detection of alien genetic introgressions in bread wheat using dot-blot genomic hybridisation
spellingShingle Detection of alien genetic introgressions in bread wheat using dot-blot genomic hybridisation
Rey-Santomé, María Dolores
Dot-blot hybridisation
Genomic introgressions
H. chilense
Bread wheat
title_short Detection of alien genetic introgressions in bread wheat using dot-blot genomic hybridisation
title_full Detection of alien genetic introgressions in bread wheat using dot-blot genomic hybridisation
title_fullStr Detection of alien genetic introgressions in bread wheat using dot-blot genomic hybridisation
title_full_unstemmed Detection of alien genetic introgressions in bread wheat using dot-blot genomic hybridisation
title_sort Detection of alien genetic introgressions in bread wheat using dot-blot genomic hybridisation
dc.creator.none.fl_str_mv Rey-Santomé, María Dolores
Prieto, Pilar
author Rey-Santomé, María Dolores
author_facet Rey-Santomé, María Dolores
Prieto, Pilar
author_role author
author2 Prieto, Pilar
author2_role author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
European Research Council
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Dot-blot hybridisation
Genomic introgressions
H. chilense
Bread wheat
topic Dot-blot hybridisation
Genomic introgressions
H. chilense
Bread wheat
description Simple, reliable methods for the identification of alien genetic introgressions are required in plant breeding programmes. The use of genomic dot-blot hybridisation allows the detection of small Hordeum chilense genomic introgressions in the descendants of genetic crosses between wheat and H. chilense addition or substitution lines in wheat when molecular markers are difficult to use. Based on genomic in situ hybridisation, DNA samples from wheat lines carrying putatively H. chilense introgressions were immobilised on a membrane, blocked with wheat genomic DNA and hybridised with biotin-labelled H. chilense genomic DNA as a probe. This dot-blot screening reduced the number of plants necessary to be analysed by molecular markers or in situ hybridisation, saving time and money. The technique was sensitive enough to detect a minimum of 5 ng of total genomic DNA immobilised on the membrane or about 1/420 dilution of H. chilense genomic DNA in the wheat background. The robustness of the technique was verified by in situ hybridisation. In addition, the detection of other wheat relative species such as Hordeum vulgare, Secale cereale and Agropyron cristatum in the wheat background was also reported.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/157118
url http://hdl.handle.net/10261/157118
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-64833-R
info:eu-repo/grantAgreement/EC/FP7/243118
http://doi.org/10.1007/s11032-017-0629-5
http://creativecommons.org/licenses/by/4.0/
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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