Evolutionary dynamics of transcriptional and translational regulation in yeast
All living organisms respond to environmental stimuli by modulating the activity of different genes. This response can occur across many locations throughout the cell such as at the level of transcription, translation, and even post-translational modifications. Some of this variation between species...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | CBUC, CESCA |
| Repositorio: | TDR. Tesis Doctorales en Red |
| OAI Identifier: | oai:www.tdx.cat:10803/668337 |
| Acceso en línea: | http://hdl.handle.net/10803/668337 |
| Access Level: | acceso abierto |
| Palabra clave: | Yeast S. cerevisiae De novo genes Phylostratigraphy Oxidative stress RNA-seq Ribo-seq Llevat Gens de novo Estrès oxidatiu 577 |
| Sumario: | All living organisms respond to environmental stimuli by modulating the activity of different genes. This response can occur across many locations throughout the cell such as at the level of transcription, translation, and even post-translational modifications. Some of this variation between species is attributable to the presence or absence of lineage-specific genes. The birth of new genes via de novo emergence from previously non-genic DNA is predicted to contribute significantly to this diversity. To explore this question, we generated high-depth transcriptomic data for 11 species of yeast, as well as ribosome profiling and proteomics data for Saccharomyces cerevisiae in rich media and oxidative stress conditions. We identified 213 putative de novo genes in S. cerevisiae; over a third of them are translated, and approximately half of them overlap more ancient genes on the antisense strand. We found that a significant fraction of S. cerevisiae genes (~5%) have emerged de novo over the past 20 million years. |
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