Regulation of DNA Methylation Patterns by CK2-Mediated Phosphorylation of Dnmt3a

DNA methylation is a central epigenetic modification that is established by de novo DNA methyltransferases. The mechanisms underlying the generation of genomic methylation patterns are still poorly understood. Using mass spectrometry and a phosphospecific Dnmt3a antibody, we demonstrate that CK2 pho...

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Detalles Bibliográficos
Autores: Deplus, Rachel, Blanchon, Loïc, Rajavelu, Arumugam, Boukaba, Abdelhalim, Defrance, Matthieu, Luciani, Judith, Rothé, Françoise, Dedeurwaerder, Sarah, Denis, Hélène, Brinkman, Arie B., Simmer, Femke, Müller, Fabian, Bertin, Benjamin, Berdasco, María, Putmans, Pascale, Calonne, Emilie, Litchfield, David W., Launoit, Yvan de, Jurkowski, Tomasz P., Stunnenberg, Hendrik G., Bock, Christoph, Sotiriou, Christos, Fraga, Mario F., Esteller, Manel, Jeltsch, Albert, Fuks, François
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/125782
Acceso en línea:https://hdl.handle.net/2445/125782
Access Level:acceso abierto
Palabra clave:Fosforilació
Metilació
ADN
Epigenètica
Phosphorylation
Methylation
DNA
Epigenetics
Descripción
Sumario:DNA methylation is a central epigenetic modification that is established by de novo DNA methyltransferases. The mechanisms underlying the generation of genomic methylation patterns are still poorly understood. Using mass spectrometry and a phosphospecific Dnmt3a antibody, we demonstrate that CK2 phosphorylates endogenous Dnmt3a at two key residues located near its PWWP domain, thereby downregulating the ability of Dnmt3a to methylate DNA. Genome-wide DNA methylation analysis shows that CK2 primarily modulates CpG methylation of several repeats, most notably of Alu SINEs. This modulation can be directly attributed to CK2-mediated phosphorylation of Dnmt3a. We also find that CK2-mediated phosphorylation is required for localization of Dnmt3a to heterochromatin. By revealing phosphorylation as a mode of regulation of de novo DNA methyltransferase function and by uncovering a mechanism for the regulation of methylation at repetitive elements, our results shed light on the origin of DNA methylation patterns.

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