Circulating lipoprotein-carried miRNome analysis reveals novel VLDL-enriched microRNAs that strongly correlate with the HDL-microRNA profile

Lipoproteins have been described as microRNAs (miRNAs) carriers. Unfortunately, the bibliography on this topic is scarce and shows a high variability between independent investigations. In addition, the miRNA profiles of the LDL and VLDL fractions have not been completely elucidated. Here, we profil...

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Autores: Rossi-Herring, Guido, Belmonte, Thalia, Rivas-Urbina,Andrea, Benítez, Sonia, Rotllan, Noemi, Crespo, Javier, Llorente-Cortés, Vicenta, Sánchez-Quesada, José Luís, Gonzalo-Calvo, David de
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/337380
Acceso en línea:http://hdl.handle.net/10261/337380
Access Level:acceso abierto
Palabra clave:HDL
LDL
Lipoprotein
MicroRNA
VLDL
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oai_identifier_str oai:digital.csic.es:10261/337380
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Circulating lipoprotein-carried miRNome analysis reveals novel VLDL-enriched microRNAs that strongly correlate with the HDL-microRNA profile
title Circulating lipoprotein-carried miRNome analysis reveals novel VLDL-enriched microRNAs that strongly correlate with the HDL-microRNA profile
spellingShingle Circulating lipoprotein-carried miRNome analysis reveals novel VLDL-enriched microRNAs that strongly correlate with the HDL-microRNA profile
Rossi-Herring, Guido
HDL
LDL
Lipoprotein
MicroRNA
VLDL
title_short Circulating lipoprotein-carried miRNome analysis reveals novel VLDL-enriched microRNAs that strongly correlate with the HDL-microRNA profile
title_full Circulating lipoprotein-carried miRNome analysis reveals novel VLDL-enriched microRNAs that strongly correlate with the HDL-microRNA profile
title_fullStr Circulating lipoprotein-carried miRNome analysis reveals novel VLDL-enriched microRNAs that strongly correlate with the HDL-microRNA profile
title_full_unstemmed Circulating lipoprotein-carried miRNome analysis reveals novel VLDL-enriched microRNAs that strongly correlate with the HDL-microRNA profile
title_sort Circulating lipoprotein-carried miRNome analysis reveals novel VLDL-enriched microRNAs that strongly correlate with the HDL-microRNA profile
dc.creator.none.fl_str_mv Rossi-Herring, Guido
Belmonte, Thalia
Rivas-Urbina,Andrea
Benítez, Sonia
Rotllan, Noemi
Crespo, Javier
Llorente-Cortés, Vicenta
Sánchez-Quesada, José Luís
Gonzalo-Calvo, David de
author Rossi-Herring, Guido
author_facet Rossi-Herring, Guido
Belmonte, Thalia
Rivas-Urbina,Andrea
Benítez, Sonia
Rotllan, Noemi
Crespo, Javier
Llorente-Cortés, Vicenta
Sánchez-Quesada, José Luís
Gonzalo-Calvo, David de
author_role author
author2 Belmonte, Thalia
Rivas-Urbina,Andrea
Benítez, Sonia
Rotllan, Noemi
Crespo, Javier
Llorente-Cortés, Vicenta
Sánchez-Quesada, José Luís
Gonzalo-Calvo, David de
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
European Commission
Generalitat de Catalunya
Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv HDL
LDL
Lipoprotein
MicroRNA
VLDL
topic HDL
LDL
Lipoprotein
MicroRNA
VLDL
description Lipoproteins have been described as microRNAs (miRNAs) carriers. Unfortunately, the bibliography on this topic is scarce and shows a high variability between independent investigations. In addition, the miRNA profiles of the LDL and VLDL fractions have not been completely elucidated. Here, we profiled the human circulating lipoprotein-carried miRNome. Lipoprotein fractions (VLDL, LDL and HDL) were isolated from the serum of healthy subjects by ultracentrifugation and purified by size-exclusion chromatography. A panel of 179 miRNAs commonly expressed in circulation was evaluated in the lipoprotein fractions using quantitative real-time PCR (qPCR) assays. A total of 14, 4 and 24 miRNAs were stably detected in the VLDL, LDL and HDL fractions, respectively. VLDL- and HDL-miRNA signatures were highly correlated (rho 0.814), and miR-16–5p, miR-142–3p, miR-223–3p and miR-451a were among the top 5 expressed miRNAs in both fractions. miR-125a-5p, miR-335–3p and miR-1260a, were detected in all lipoprotein fractions. miR-107 and miR-221–3p were uniquely detected in the VLDL fraction. HDL showed the larger number of specifically detected miRNAs (n = 13). Enrichment in specific miRNA families and genomic clusters was observed for HDL-miRNAs. Two sequence motifs were also detected for this group of miRNAs. Functional enrichment analysis including the miRNA signatures from each lipoprotein fraction suggested a potential role in mechanistic pathways previously associated with cardiovascular disease: fibrosis, senescence, inflammation, immune response, angiogenesis, and cardiomyopathy. Collectively, our results not only support the role of lipoproteins as circulating miRNA carriers but also describe for the first time the role of VLDL as a miRNA transporter.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/337380
url http://hdl.handle.net/10261/337380
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104367RB-I00
Rossi-Herring, Guido; Belmonte, Thalia; Rivas-Urbina,Andrea; Benítez, Sonia; Rotllan, Noemi; Crespo, Javier; Llorente-Cortés, Vicenta; Sánchez-Quesada, José Luís; Gonzalo-Calvo, David de; 2023; Supplementary Material Circulating lipoprotein-carried miRNome analysis reveals novel VLDL-enriched microRNAs that strongly correlate with the HDL-microRNA profile [Dataset]; Elsevier; https://doi.org/10.1016/j.biopha.2023.114623
http://dx.doi.org/10.1016/j.biopha.2023.114623
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Circulating lipoprotein-carried miRNome analysis reveals novel VLDL-enriched microRNAs that strongly correlate with the HDL-microRNA profileRossi-Herring, GuidoBelmonte, ThaliaRivas-Urbina,AndreaBenítez, SoniaRotllan, NoemiCrespo, JavierLlorente-Cortés, VicentaSánchez-Quesada, José LuísGonzalo-Calvo, David deHDLLDLLipoproteinMicroRNAVLDLLipoproteins have been described as microRNAs (miRNAs) carriers. Unfortunately, the bibliography on this topic is scarce and shows a high variability between independent investigations. In addition, the miRNA profiles of the LDL and VLDL fractions have not been completely elucidated. Here, we profiled the human circulating lipoprotein-carried miRNome. Lipoprotein fractions (VLDL, LDL and HDL) were isolated from the serum of healthy subjects by ultracentrifugation and purified by size-exclusion chromatography. A panel of 179 miRNAs commonly expressed in circulation was evaluated in the lipoprotein fractions using quantitative real-time PCR (qPCR) assays. A total of 14, 4 and 24 miRNAs were stably detected in the VLDL, LDL and HDL fractions, respectively. VLDL- and HDL-miRNA signatures were highly correlated (rho 0.814), and miR-16–5p, miR-142–3p, miR-223–3p and miR-451a were among the top 5 expressed miRNAs in both fractions. miR-125a-5p, miR-335–3p and miR-1260a, were detected in all lipoprotein fractions. miR-107 and miR-221–3p were uniquely detected in the VLDL fraction. HDL showed the larger number of specifically detected miRNAs (n = 13). Enrichment in specific miRNA families and genomic clusters was observed for HDL-miRNAs. Two sequence motifs were also detected for this group of miRNAs. Functional enrichment analysis including the miRNA signatures from each lipoprotein fraction suggested a potential role in mechanistic pathways previously associated with cardiovascular disease: fibrosis, senescence, inflammation, immune response, angiogenesis, and cardiomyopathy. Collectively, our results not only support the role of lipoproteins as circulating miRNA carriers but also describe for the first time the role of VLDL as a miRNA transporter.This study has been funded by Instituto de Salud Carlos III (ISCIII) through the project "Miguel Servet 2020: CP20/00041" and co-funded by the European Union. This work is supported by Instituto de Salud Carlos III (PI16/00471, PI20/00334, PI20/00577 and PI21/01523), co-funded by the European Union, IRBLleida - Fundació Dr. Pifarré, CERCA Programme/Generalitat de Catalunya. NR has received financial support from Ministerio de Ciencia, Innovación y Universidades (PID2019-104367RB-100), and Agencia Estatal de Investigación (AEI/10.13039/501100011033) within the Subprograma Ramón y Cajal (RYC-201722879). CIBERDEM (CB07/08/0016), CIBERES (CB07/06/2008) and CIBERCV (CB16/11/00276) are initiatives of the Instituto de Salud Carlos III.ElsevierInstituto de Salud Carlos IIIEuropean CommissionGeneralitat de CatalunyaMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2023202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/337380reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104367RB-I00Rossi-Herring, Guido; Belmonte, Thalia; Rivas-Urbina,Andrea; Benítez, Sonia; Rotllan, Noemi; Crespo, Javier; Llorente-Cortés, Vicenta; Sánchez-Quesada, José Luís; Gonzalo-Calvo, David de; 2023; Supplementary Material Circulating lipoprotein-carried miRNome analysis reveals novel VLDL-enriched microRNAs that strongly correlate with the HDL-microRNA profile [Dataset]; Elsevier; https://doi.org/10.1016/j.biopha.2023.114623http://dx.doi.org/10.1016/j.biopha.2023.114623Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3373802026-05-22T06:33:51Z
score 15,811543

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