Centrosome clustering and cyclin D1 gene amplification in double minutes are common events in chromosomal

Background: Aneuploidy, centrosome abnormalities and gene amplification are hallmarks of chromosome instability (CIN) in cancer. Yet there are no studies of the in vivo behavior of these phenomena within the same bladder tumor. Methods: Twenty-one paraffin-embedded bladder tumors were analyzed by co...

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Authors: del Rey, Javier, Prat, Esther, Ponsa, Immaculada, Lloreta, Josep, 1958-, Gelabert, Antoni, Algaba, Ferran, Camps-Puchadas, Jordi, Miró, Rosa
Format: article
Status:Published version
Publication Date:2010
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/23397
Online Access:http://hdl.handle.net/10230/23397
http://dx.doi.org/10.1186/1471-2407-10-280
Access Level:Open access
Keyword:Bufeta -- Càncer
Carcinogènesi
Mitosi
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oai_identifier_str oai:recercat.cat:10230/23397
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spelling Centrosome clustering and cyclin D1 gene amplification in double minutes are common events in chromosomaldel Rey, JavierPrat, EstherPonsa, ImmaculadaLloreta, Josep, 1958-Gelabert, AntoniAlgaba, FerranCamps-Puchadas, JordiMiró, RosaBufeta -- CàncerCarcinogènesiMitosiBackground: Aneuploidy, centrosome abnormalities and gene amplification are hallmarks of chromosome instability (CIN) in cancer. Yet there are no studies of the in vivo behavior of these phenomena within the same bladder tumor. Methods: Twenty-one paraffin-embedded bladder tumors were analyzed by conventional comparative genome hybridization and fluorescence in situ hybridization (FISH) with a cyclin D1 gene (CCND1)/centromere 11 dual-color probe. Immunofluorescent staining of α, β and γ tubulin was also performed. Results: Based on the CIN index, defined as the percentage of cells not displaying the modal number for chromosome 11, tumors were classified as CIN-negative and CIN-positive. Fourteen out of 21 tumors were considered CIN-positive. All T1G3 tumors were included in the CIN-positive group whereas the majority of Ta samples were classified as CIN-negative tumors. Centrosome clustering was observed in six out of 12 CIN-positive tumors analyzed. CCND1 amplification in homogeneously staining regions was present in six out of 14 CIN-positive tumors; three of them also showed amplification of this gene in double minutes. Conclusions: Complex in vivo behavior of CCND1 amplicon in bladder tumor cells has been demonstrated by accurate FISH analysis on paraffin-embedded tumors. Positive correlation between high heterogeneity, centrosome abnormalities and CCND1 amplification was found in T1G3 bladder carcinomas. This is the first study to provide insights into the coexistence of CCND1 amplification in homogeneously staining regions and double minutes in primary bladder tumors. It is noteworthy that those patients whose tumors showed double minutes had a significantly shorter overall survival rate (p < 0.001).The work has been supported by ISCIII: EPICUR-Red (FIS G03/174), SAF2007-64167 and RD06/0020/1020. Javier del Rey was a fellow of Generalitat de Catalunya and is supported by RD06/0020/1020BioMed Central201520152010info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/23397http://dx.doi.org/10.1186/1471-2407-10-280reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésBMC Cancer. 2010;10:280info:eu-repo/grantAgreement/ES/2PN/SAF2007-64167© del Rey J, Prat E, Ponsa I, Lloreta J, Gelabert A, Algaba F, Camps J, Miró R. Creative Commons Attribution License http://creativecommons.org/licenses/by/2.0/http://creativecommons.org/licenses/by/2.0info:eu-repo/semantics/openAccessoai:recercat.cat:10230/233972026-05-29T05:05:01Z
dc.title.none.fl_str_mv Centrosome clustering and cyclin D1 gene amplification in double minutes are common events in chromosomal
title Centrosome clustering and cyclin D1 gene amplification in double minutes are common events in chromosomal
spellingShingle Centrosome clustering and cyclin D1 gene amplification in double minutes are common events in chromosomal
del Rey, Javier
Bufeta -- Càncer
Carcinogènesi
Mitosi
title_short Centrosome clustering and cyclin D1 gene amplification in double minutes are common events in chromosomal
title_full Centrosome clustering and cyclin D1 gene amplification in double minutes are common events in chromosomal
title_fullStr Centrosome clustering and cyclin D1 gene amplification in double minutes are common events in chromosomal
title_full_unstemmed Centrosome clustering and cyclin D1 gene amplification in double minutes are common events in chromosomal
title_sort Centrosome clustering and cyclin D1 gene amplification in double minutes are common events in chromosomal
dc.creator.none.fl_str_mv del Rey, Javier
Prat, Esther
Ponsa, Immaculada
Lloreta, Josep, 1958-
Gelabert, Antoni
Algaba, Ferran
Camps-Puchadas, Jordi
Miró, Rosa
author del Rey, Javier
author_facet del Rey, Javier
Prat, Esther
Ponsa, Immaculada
Lloreta, Josep, 1958-
Gelabert, Antoni
Algaba, Ferran
Camps-Puchadas, Jordi
Miró, Rosa
author_role author
author2 Prat, Esther
Ponsa, Immaculada
Lloreta, Josep, 1958-
Gelabert, Antoni
Algaba, Ferran
Camps-Puchadas, Jordi
Miró, Rosa
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bufeta -- Càncer
Carcinogènesi
Mitosi
topic Bufeta -- Càncer
Carcinogènesi
Mitosi
description Background: Aneuploidy, centrosome abnormalities and gene amplification are hallmarks of chromosome instability (CIN) in cancer. Yet there are no studies of the in vivo behavior of these phenomena within the same bladder tumor. Methods: Twenty-one paraffin-embedded bladder tumors were analyzed by conventional comparative genome hybridization and fluorescence in situ hybridization (FISH) with a cyclin D1 gene (CCND1)/centromere 11 dual-color probe. Immunofluorescent staining of α, β and γ tubulin was also performed. Results: Based on the CIN index, defined as the percentage of cells not displaying the modal number for chromosome 11, tumors were classified as CIN-negative and CIN-positive. Fourteen out of 21 tumors were considered CIN-positive. All T1G3 tumors were included in the CIN-positive group whereas the majority of Ta samples were classified as CIN-negative tumors. Centrosome clustering was observed in six out of 12 CIN-positive tumors analyzed. CCND1 amplification in homogeneously staining regions was present in six out of 14 CIN-positive tumors; three of them also showed amplification of this gene in double minutes. Conclusions: Complex in vivo behavior of CCND1 amplicon in bladder tumor cells has been demonstrated by accurate FISH analysis on paraffin-embedded tumors. Positive correlation between high heterogeneity, centrosome abnormalities and CCND1 amplification was found in T1G3 bladder carcinomas. This is the first study to provide insights into the coexistence of CCND1 amplification in homogeneously staining regions and double minutes in primary bladder tumors. It is noteworthy that those patients whose tumors showed double minutes had a significantly shorter overall survival rate (p < 0.001).
publishDate 2010
dc.date.none.fl_str_mv 2010
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/23397
http://dx.doi.org/10.1186/1471-2407-10-280
url http://hdl.handle.net/10230/23397
http://dx.doi.org/10.1186/1471-2407-10-280
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv BMC Cancer. 2010;10:280
info:eu-repo/grantAgreement/ES/2PN/SAF2007-64167
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/2.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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