Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS/dsRNA-loaded nanoliposomes

Under a Creative Commons license.-- et al.

Detalhes bibliográficos
Autores: Ruyra, Angels, Cano-Sarabia, Mary, MacKenzie, Simon, Maspoch, Daniel, Roher, Nerea
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/127201
Acesso em linha:http://hdl.handle.net/10261/127201
Access Level:acceso abierto
Palavra-chave:Innate immunity
Zebrafish
Infection models
Immunostimulant
Nanoencapsulation
Liposome
Macrophage
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spelling Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS/dsRNA-loaded nanoliposomesRuyra, AngelsCano-Sarabia, MaryMacKenzie, SimonMaspoch, DanielRoher, NereaInnate immunityZebrafishInfection modelsImmunostimulantNanoencapsulationLiposomeMacrophageUnder a Creative Commons license.-- et al.Herein we report the use of immunostimulant-loaded nanoliposomes (called NLc liposomes) as a strategy to protect fish against bacterial and/or viral infections. This work entailed developing a method for in vivo tracking of the liposomes administered to adult zebrafish that enables evaluation of their in vivo dynamics and characterisation of their tissue distribution. The NLc liposomes, which co-encapsulate poly(I:C) and LPS, accumulate in immune tissues and in immunologically relevant cells such as macrophages, as has been assessed in trout primary cell cultures. They protect zebrafish against otherwise lethal bacterial (Pseudomonas aeruginosa PAO1) and viral (Spring Viraemia of Carp Virus) infections regardless of whether they are administered by injection or by immersion, as demonstrated in a series of in vivo infection experiments with adult zebrafish. Importantly, protection was not achieved in fish that had been treated with empty liposomes or with a mixture of the free immunostimulants. Our findings indicate that stimulation of the innate immune system with co-encapsulated immunostimulants in nano-liposomes is a promising strategy to simultaneously improve the levels of protection against bacterial and viral infections in fish.The authors acknowledge financial support from Fundación Ramon Areces, AGL2012-33877 (MINECO, Spain) and Aposta (UAB). AR thanks Fundación Ramon Areces for a PhD fellowship and NR thanks MINECO for a Ramón y Cajal grant.Peer ReviewedElsevierMinisterio de Economía y Competitividad (España)Fundación Ramón ArecesUniversidad Autónoma de BarcelonaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2015201520142015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/127201reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.vaccine.2014.05.010Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1272012026-05-22T06:33:51Z
dc.title.none.fl_str_mv Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS/dsRNA-loaded nanoliposomes
title Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS/dsRNA-loaded nanoliposomes
spellingShingle Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS/dsRNA-loaded nanoliposomes
Ruyra, Angels
Innate immunity
Zebrafish
Infection models
Immunostimulant
Nanoencapsulation
Liposome
Macrophage
title_short Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS/dsRNA-loaded nanoliposomes
title_full Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS/dsRNA-loaded nanoliposomes
title_fullStr Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS/dsRNA-loaded nanoliposomes
title_full_unstemmed Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS/dsRNA-loaded nanoliposomes
title_sort Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS/dsRNA-loaded nanoliposomes
dc.creator.none.fl_str_mv Ruyra, Angels
Cano-Sarabia, Mary
MacKenzie, Simon
Maspoch, Daniel
Roher, Nerea
author Ruyra, Angels
author_facet Ruyra, Angels
Cano-Sarabia, Mary
MacKenzie, Simon
Maspoch, Daniel
Roher, Nerea
author_role author
author2 Cano-Sarabia, Mary
MacKenzie, Simon
Maspoch, Daniel
Roher, Nerea
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Fundación Ramón Areces
Universidad Autónoma de Barcelona
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Innate immunity
Zebrafish
Infection models
Immunostimulant
Nanoencapsulation
Liposome
Macrophage
topic Innate immunity
Zebrafish
Infection models
Immunostimulant
Nanoencapsulation
Liposome
Macrophage
description Under a Creative Commons license.-- et al.
publishDate 2014
dc.date.none.fl_str_mv 2014
2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/127201
url http://hdl.handle.net/10261/127201
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.vaccine.2014.05.010
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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