Untargeted mutagenesis of brassinosteroid receptor SbBRI1 confers drought tolerance by altering phenylpropanoid metabolism in Sorghum bicolor
Drought is a critical issue in modern agriculture; therefore, there is a need to create crops withdrought resilience. The complexity of plant responses to abiotic stresses, particularly in the fieldof brassinosteroid (BR) signalling, has been the subject of extensive research. In this study, weunveil...
| Autores: | , , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/378256 |
| Acceso en línea: | http://hdl.handle.net/10261/378256 https://api.elsevier.com/content/abstract/scopus_id/85205288147 |
| Access Level: | acceso abierto |
| Palabra clave: | Sorghum bicolor Drought Brassinosteroids BRI1 Phenylpropanoid DAP-seq |
| Sumario: | Drought is a critical issue in modern agriculture; therefore, there is a need to create crops withdrought resilience. The complexity of plant responses to abiotic stresses, particularly in the fieldof brassinosteroid (BR) signalling, has been the subject of extensive research. In this study, weunveil compelling insights indicating that the BRASSINOSTEROID-INSENSITIVE 1 (BRI1) receptor inArabidopsis and Sorghum plays a critical role as a negative regulator of drought responses.Introducing untargeted mutation in the sorghum BRI1 receptor (SbBRI1) effectively enhances theplant’s ability to withstand osmotic and drought stress. Through DNA Affinity Purificationsequencing (DAP-seq), we show that the sorghum BRI1-EMS-SUPPRESSOR 1 (SbBES1)transcription factor, a downstream player of the BR signalling, binds to a conserved G-boxbinding motif, and it is responsible for regulating BR homeostasis, as its Arabidopsis orthologAtBES1. We further characterized the drought tolerance of sorghum bri1 mutants and decipherSbBES1-mediated regulation of phenylpropanoid pathway. Our findings suggest that SbBRI1signalling serves a dual purpose: under normal conditions, it regulates lignin biosynthesis bySbBES1, but during drought conditions, BES1 becomes less active, allowing the activation of theflavonoid pathway. This adaptive shift improves the photosynthetic rate and photoprotection,reinforcing crop adaptation to drought. |
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