Design and Validation of qPCR-Specific Primers for Quantification of the Marketed Terfezia claveryi and Terfezia crassiverrucosa in Soil

Desert truffle crop is a pioneer in southeastern Spain, a region where native edible hypogeous fungi are adapted to the semiarid areas with low annual rainfall. Terfezia claveryi Chatin was the first species of desert truffle to be cultivated, and has been increasing in recent years as an alternativ...

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Autores: Arenas, Francisco, Morte, Asunción, Navarro-Ródenas, Alfonso
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/466357
Acceso en línea:https://doi.org/10.1016/j.foreco.2022.120602
https://hdl.handle.net/10459.1/466357
Access Level:acceso abierto
Palabra clave:ITS
Terfezia claveryi
Terfezia crassiverrucosa
qPCR
Desert truffles
rDNA
Ascomycetes
mycelium
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spelling Design and Validation of qPCR-Specific Primers for Quantification of the Marketed Terfezia claveryi and Terfezia crassiverrucosa in SoilArenas, FranciscoMorte, AsunciónNavarro-Ródenas, AlfonsoITSTerfezia claveryiTerfezia crassiverrucosaqPCRDesert trufflesrDNAAscomycetesmyceliumDesert truffle crop is a pioneer in southeastern Spain, a region where native edible hypogeous fungi are adapted to the semiarid areas with low annual rainfall. Terfezia claveryi Chatin was the first species of desert truffle to be cultivated, and has been increasing in recent years as an alternative rainfed crop in the Iberian Peninsula. However, its behaviour in the field has yet not been investigated. For this purpose, specific primers were designed for the soil DNA quantification of both T. claveryi and Terfezia crassiverrucosa and a real-time qPCR protocol was developed, using the ITS rDNA region as a target. Moreover, a young desert truffle orchard was sampled for environmental validation. The results showed the highest efficiency for the TerclaF3/TerclaR1 primers pair, 89%, and the minimal fungal biomass that could be reliable detected was set at 4.23 µg mycelium/g soil. The spatial distribution of fungal biomass was heterogeneous, and there was not a direct relationship between the quantity of winter soil mycelium and the location/productivity of desert truffles. This protocol could be applied to tracking these species in soil and understand their mycelial dynamics in plantations and wild areas.This research was funded by MCIN/AEI/10.13039/50110001103, grant number PID2020- 115210RB-I00, and FEDER and Fundación Séneca, Agencia de Ciencia y Tecnología of the Region of Murcia (Spain), grant number 20866/PI/18.MDPI2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.1016/j.foreco.2022.120602https://hdl.handle.net/10459.1/466357reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)Inglésinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115210RB-I00Reproducció del document publicat a https://doi.org/10.1016/j.foreco.2022.120602Journal of Fungi, 2022, vol. 8, núm. 10, art. 1095cc-by (c) The Authors, 2022Attribution 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:repositori.udl.cat:10459.1/4663572026-06-24T12:42:17Z
dc.title.none.fl_str_mv Design and Validation of qPCR-Specific Primers for Quantification of the Marketed Terfezia claveryi and Terfezia crassiverrucosa in Soil
title Design and Validation of qPCR-Specific Primers for Quantification of the Marketed Terfezia claveryi and Terfezia crassiverrucosa in Soil
spellingShingle Design and Validation of qPCR-Specific Primers for Quantification of the Marketed Terfezia claveryi and Terfezia crassiverrucosa in Soil
Arenas, Francisco
ITS
Terfezia claveryi
Terfezia crassiverrucosa
qPCR
Desert truffles
rDNA
Ascomycetes
mycelium
title_short Design and Validation of qPCR-Specific Primers for Quantification of the Marketed Terfezia claveryi and Terfezia crassiverrucosa in Soil
title_full Design and Validation of qPCR-Specific Primers for Quantification of the Marketed Terfezia claveryi and Terfezia crassiverrucosa in Soil
title_fullStr Design and Validation of qPCR-Specific Primers for Quantification of the Marketed Terfezia claveryi and Terfezia crassiverrucosa in Soil
title_full_unstemmed Design and Validation of qPCR-Specific Primers for Quantification of the Marketed Terfezia claveryi and Terfezia crassiverrucosa in Soil
title_sort Design and Validation of qPCR-Specific Primers for Quantification of the Marketed Terfezia claveryi and Terfezia crassiverrucosa in Soil
dc.creator.none.fl_str_mv Arenas, Francisco
Morte, Asunción
Navarro-Ródenas, Alfonso
author Arenas, Francisco
author_facet Arenas, Francisco
Morte, Asunción
Navarro-Ródenas, Alfonso
author_role author
author2 Morte, Asunción
Navarro-Ródenas, Alfonso
author2_role author
author
dc.subject.none.fl_str_mv ITS
Terfezia claveryi
Terfezia crassiverrucosa
qPCR
Desert truffles
rDNA
Ascomycetes
mycelium
topic ITS
Terfezia claveryi
Terfezia crassiverrucosa
qPCR
Desert truffles
rDNA
Ascomycetes
mycelium
description Desert truffle crop is a pioneer in southeastern Spain, a region where native edible hypogeous fungi are adapted to the semiarid areas with low annual rainfall. Terfezia claveryi Chatin was the first species of desert truffle to be cultivated, and has been increasing in recent years as an alternative rainfed crop in the Iberian Peninsula. However, its behaviour in the field has yet not been investigated. For this purpose, specific primers were designed for the soil DNA quantification of both T. claveryi and Terfezia crassiverrucosa and a real-time qPCR protocol was developed, using the ITS rDNA region as a target. Moreover, a young desert truffle orchard was sampled for environmental validation. The results showed the highest efficiency for the TerclaF3/TerclaR1 primers pair, 89%, and the minimal fungal biomass that could be reliable detected was set at 4.23 µg mycelium/g soil. The spatial distribution of fungal biomass was heterogeneous, and there was not a direct relationship between the quantity of winter soil mycelium and the location/productivity of desert truffles. This protocol could be applied to tracking these species in soil and understand their mycelial dynamics in plantations and wild areas.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.foreco.2022.120602
https://hdl.handle.net/10459.1/466357
url https://doi.org/10.1016/j.foreco.2022.120602
https://hdl.handle.net/10459.1/466357
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115210RB-I00
Reproducció del document publicat a https://doi.org/10.1016/j.foreco.2022.120602
Journal of Fungi, 2022, vol. 8, núm. 10, art. 1095
dc.rights.none.fl_str_mv cc-by (c) The Authors, 2022
Attribution 4.0 International
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by (c) The Authors, 2022
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
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