Selenite-induced cell death in Saccharomyces cerevisiae: protective role of glutaredoxins

Unlike in higher organisms, selenium is not essential for growth in Saccharomyces cerevisiae. In this species, it causes toxic effects at high concentrations. In the present study, we show that when supplied as selenite to yeast cultures growing under fermentative metabolism, its effects can be diss...

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Autores: Izquierdo Álvarez, Alicia, Casas Herranz, Celia, Herrero Perpiñán, Enrique
Tipo de recurso: artículo
Estado:Versión enviada para evaluación y publicación
Fecha de publicación:2010
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10459.1/47444
Acceso en línea:https://doi.org/10.1099/mic.0.039719-0
http://hdl.handle.net/10459.1/47444
Access Level:acceso abierto
Palabra clave:Llevat de cervesa
Glutaredoxin
Estrès oxidatiu
Saccharomyces cerevisiae
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spelling Selenite-induced cell death in Saccharomyces cerevisiae: protective role of glutaredoxinsIzquierdo Álvarez, AliciaCasas Herranz, CeliaHerrero Perpiñán, EnriqueLlevat de cervesaGlutaredoxinEstrès oxidatiuSaccharomyces cerevisiaeEstrès oxidatiuUnlike in higher organisms, selenium is not essential for growth in Saccharomyces cerevisiae. In this species, it causes toxic effects at high concentrations. In the present study, we show that when supplied as selenite to yeast cultures growing under fermentative metabolism, its effects can be dissected into two death phases. From the time of initial treatment, it causes loss of membrane integrity and genotoxicity. Both effects occur at higher levels in mutants lacking Grx1p and Grx2p than in wild-type cells, and are reversed by expression of a cytosolic version of the membrane-associated Grx7p glutaredoxin. Grx7p can also rescue the high levels of protein carbonylation damage that occur in selenite-treated cultures of the grx1 grx2 mutant. After longer incubation times, selenite causes abnormal nuclear morphology and the appearance of TUNEL-positive cells, which are considered apoptotic markers in yeast cells. This effect is independent of Grx1p and Grx2p. Therefore, the protective role of the two glutaredoxins is restricted to the initial stages of selenite treatment. Lack of Yca1p metacaspase or of a functional mitochondrial electron transport chain only moderately diminishes apoptotic-like death by selenite. In contrast, selenite-induced apoptosis is dependent on the apoptosis-inducing factor Aif1p. In the absence of the latter, intracellular protein carbonylation is reduced after prolonged selenite treatment, supporting the supposition that part of the oxidative damage is contributed by apoptotic cells.Society for General Microbiology2010info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttps://doi.org/10.1099/mic.0.039719-0http://hdl.handle.net/10459.1/47444reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió preprint del document publicat a: https://doi.org/10.1099/mic.0.039719-0Microbiology, 2010, vol. 156, núm. 9, p. 2608-2620(c) Society for General Microbiologyinfo:eu-repo/semantics/openAccessoai:recercat.cat:10459.1/474442026-05-29T05:05:01Z
dc.title.none.fl_str_mv Selenite-induced cell death in Saccharomyces cerevisiae: protective role of glutaredoxins
title Selenite-induced cell death in Saccharomyces cerevisiae: protective role of glutaredoxins
spellingShingle Selenite-induced cell death in Saccharomyces cerevisiae: protective role of glutaredoxins
Izquierdo Álvarez, Alicia
Llevat de cervesa
Glutaredoxin
Estrès oxidatiu
Saccharomyces cerevisiae
Estrès oxidatiu
title_short Selenite-induced cell death in Saccharomyces cerevisiae: protective role of glutaredoxins
title_full Selenite-induced cell death in Saccharomyces cerevisiae: protective role of glutaredoxins
title_fullStr Selenite-induced cell death in Saccharomyces cerevisiae: protective role of glutaredoxins
title_full_unstemmed Selenite-induced cell death in Saccharomyces cerevisiae: protective role of glutaredoxins
title_sort Selenite-induced cell death in Saccharomyces cerevisiae: protective role of glutaredoxins
dc.creator.none.fl_str_mv Izquierdo Álvarez, Alicia
Casas Herranz, Celia
Herrero Perpiñán, Enrique
author Izquierdo Álvarez, Alicia
author_facet Izquierdo Álvarez, Alicia
Casas Herranz, Celia
Herrero Perpiñán, Enrique
author_role author
author2 Casas Herranz, Celia
Herrero Perpiñán, Enrique
author2_role author
author
dc.subject.none.fl_str_mv Llevat de cervesa
Glutaredoxin
Estrès oxidatiu
Saccharomyces cerevisiae
Estrès oxidatiu
topic Llevat de cervesa
Glutaredoxin
Estrès oxidatiu
Saccharomyces cerevisiae
Estrès oxidatiu
description Unlike in higher organisms, selenium is not essential for growth in Saccharomyces cerevisiae. In this species, it causes toxic effects at high concentrations. In the present study, we show that when supplied as selenite to yeast cultures growing under fermentative metabolism, its effects can be dissected into two death phases. From the time of initial treatment, it causes loss of membrane integrity and genotoxicity. Both effects occur at higher levels in mutants lacking Grx1p and Grx2p than in wild-type cells, and are reversed by expression of a cytosolic version of the membrane-associated Grx7p glutaredoxin. Grx7p can also rescue the high levels of protein carbonylation damage that occur in selenite-treated cultures of the grx1 grx2 mutant. After longer incubation times, selenite causes abnormal nuclear morphology and the appearance of TUNEL-positive cells, which are considered apoptotic markers in yeast cells. This effect is independent of Grx1p and Grx2p. Therefore, the protective role of the two glutaredoxins is restricted to the initial stages of selenite treatment. Lack of Yca1p metacaspase or of a functional mitochondrial electron transport chain only moderately diminishes apoptotic-like death by selenite. In contrast, selenite-induced apoptosis is dependent on the apoptosis-inducing factor Aif1p. In the absence of the latter, intracellular protein carbonylation is reduced after prolonged selenite treatment, supporting the supposition that part of the oxidative damage is contributed by apoptotic cells.
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1099/mic.0.039719-0
http://hdl.handle.net/10459.1/47444
url https://doi.org/10.1099/mic.0.039719-0
http://hdl.handle.net/10459.1/47444
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió preprint del document publicat a: https://doi.org/10.1099/mic.0.039719-0
Microbiology, 2010, vol. 156, núm. 9, p. 2608-2620
dc.rights.none.fl_str_mv (c) Society for General Microbiology
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Society for General Microbiology
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Society for General Microbiology
publisher.none.fl_str_mv Society for General Microbiology
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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