Cerium (III) and (IV) contammg mesoporous glasses/alginate beads for bone regeneration: Bioactivity, biocompatibility and reactive oxygen species activity.

A very small number of biomaterials investigated for bone regeneration was reported as able to prevent the oxidative stress. In this study beads based on alginate hydrogel and mesoporous glasses (MG) containing different amounts of cerium oxides (Ce3+/Ce4+) exhibiting antioxidant properties were inv...

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Detalles Bibliográficos
Autores: Varini, E., Sánchez Salcedo, Sandra, Malavasi, Gianluca, Lusvardi, Gigliola, Vallet Regí, María Dulce Nombre, Salinas Sánchez, Antonio Jesús
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:español
OAI Identifier:oai:docta.ucm.es:20.500.14352/13464
Acceso en línea:https://hdl.handle.net/20.500.14352/13464
Access Level:acceso abierto
Palabra clave:579
Mesoporous glasses
Cenum (III) and (IV)
Alginate
Beads
Oxidative stress
Bone regeneration
Materiales
3312 Tecnología de Materiales
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oai_identifier_str oai:docta.ucm.es:20.500.14352/13464
network_acronym_str ES
network_name_str España
repository_id_str
spelling Cerium (III) and (IV) contammg mesoporous glasses/alginate beads for bone regeneration: Bioactivity, biocompatibility and reactive oxygen species activity.Varini, E.Sánchez Salcedo, SandraMalavasi, GianlucaLusvardi, GigliolaVallet Regí, María Dulce NombreSalinas Sánchez, Antonio Jesús579Mesoporous glassesCenum (III) and (IV)AlginateBeadsOxidative stressBone regenerationMateriales3312 Tecnología de MaterialesA very small number of biomaterials investigated for bone regeneration was reported as able to prevent the oxidative stress. In this study beads based on alginate hydrogel and mesoporous glasses (MG) containing different amounts of cerium oxides (Ce3+/Ce4+) exhibiting antioxidant properties were investigated as a good approach to mimic the action of antioxidant enzymes in our organism. The effect of cerium contents on the bioactivity and biocompatibility of beads were investigated. Moreover, the potential capability of Ce-containing MG to prevent the oxidative stress caused by the activity of reactive oxygen species (ROS) was here investigated for the first time. The increment of cerium oxide from 1.2, to 3.6 and 5.3 mol-% decreases the surface area and porosity of MG and increases the catalase mimetic activity after 168 h. Swelling tests in different cell culture media (D­ and u-MEM) demonstrated the rehydration capability of beads. The presence of beads with the highest Ce-contents (3.6 and 5.3 %) improved the proliferation of pre-osteoblastic cells MC3T3-Cl cells. However, the cell differentiation decreased when increased the cerium content. Lactate dehydrogenase assays showed beads are cytocompatible materials. Moreover, oxidative stress tests with H zO z showed a better response related to cell viability and the elimination of oxidant species when increased cerium content. Beads of glasses with 1.2 and 3.6 % of Ce02 are excellent candidates as bioactive scaffolds for bone regeneration capable of counteract the oxidative stress.ElsevierUniversidad Complutense de Madrid20192019-07-1520192019-07-15journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/13464reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Españolspaopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/134642026-06-02T12:44:21Z
dc.title.none.fl_str_mv Cerium (III) and (IV) contammg mesoporous glasses/alginate beads for bone regeneration: Bioactivity, biocompatibility and reactive oxygen species activity.
title Cerium (III) and (IV) contammg mesoporous glasses/alginate beads for bone regeneration: Bioactivity, biocompatibility and reactive oxygen species activity.
spellingShingle Cerium (III) and (IV) contammg mesoporous glasses/alginate beads for bone regeneration: Bioactivity, biocompatibility and reactive oxygen species activity.
Varini, E.
579
Mesoporous glasses
Cenum (III) and (IV)
Alginate
Beads
Oxidative stress
Bone regeneration
Materiales
3312 Tecnología de Materiales
title_short Cerium (III) and (IV) contammg mesoporous glasses/alginate beads for bone regeneration: Bioactivity, biocompatibility and reactive oxygen species activity.
title_full Cerium (III) and (IV) contammg mesoporous glasses/alginate beads for bone regeneration: Bioactivity, biocompatibility and reactive oxygen species activity.
title_fullStr Cerium (III) and (IV) contammg mesoporous glasses/alginate beads for bone regeneration: Bioactivity, biocompatibility and reactive oxygen species activity.
title_full_unstemmed Cerium (III) and (IV) contammg mesoporous glasses/alginate beads for bone regeneration: Bioactivity, biocompatibility and reactive oxygen species activity.
title_sort Cerium (III) and (IV) contammg mesoporous glasses/alginate beads for bone regeneration: Bioactivity, biocompatibility and reactive oxygen species activity.
dc.creator.none.fl_str_mv Varini, E.
Sánchez Salcedo, Sandra
Malavasi, Gianluca
Lusvardi, Gigliola
Vallet Regí, María Dulce Nombre
Salinas Sánchez, Antonio Jesús
author Varini, E.
author_facet Varini, E.
Sánchez Salcedo, Sandra
Malavasi, Gianluca
Lusvardi, Gigliola
Vallet Regí, María Dulce Nombre
Salinas Sánchez, Antonio Jesús
author_role author
author2 Sánchez Salcedo, Sandra
Malavasi, Gianluca
Lusvardi, Gigliola
Vallet Regí, María Dulce Nombre
Salinas Sánchez, Antonio Jesús
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 579
Mesoporous glasses
Cenum (III) and (IV)
Alginate
Beads
Oxidative stress
Bone regeneration
Materiales
3312 Tecnología de Materiales
topic 579
Mesoporous glasses
Cenum (III) and (IV)
Alginate
Beads
Oxidative stress
Bone regeneration
Materiales
3312 Tecnología de Materiales
description A very small number of biomaterials investigated for bone regeneration was reported as able to prevent the oxidative stress. In this study beads based on alginate hydrogel and mesoporous glasses (MG) containing different amounts of cerium oxides (Ce3+/Ce4+) exhibiting antioxidant properties were investigated as a good approach to mimic the action of antioxidant enzymes in our organism. The effect of cerium contents on the bioactivity and biocompatibility of beads were investigated. Moreover, the potential capability of Ce-containing MG to prevent the oxidative stress caused by the activity of reactive oxygen species (ROS) was here investigated for the first time. The increment of cerium oxide from 1.2, to 3.6 and 5.3 mol-% decreases the surface area and porosity of MG and increases the catalase mimetic activity after 168 h. Swelling tests in different cell culture media (D­ and u-MEM) demonstrated the rehydration capability of beads. The presence of beads with the highest Ce-contents (3.6 and 5.3 %) improved the proliferation of pre-osteoblastic cells MC3T3-Cl cells. However, the cell differentiation decreased when increased the cerium content. Lactate dehydrogenase assays showed beads are cytocompatible materials. Moreover, oxidative stress tests with H zO z showed a better response related to cell viability and the elimination of oxidant species when increased cerium content. Beads of glasses with 1.2 and 3.6 % of Ce02 are excellent candidates as bioactive scaffolds for bone regeneration capable of counteract the oxidative stress.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-07-15
2019
2019-07-15
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/13464
url https://hdl.handle.net/20.500.14352/13464
dc.language.none.fl_str_mv Español
spa
language_invalid_str_mv Español
language spa
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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