Actin reorganization at the centrosomal area and the immune synapse regulates polarized secretory traffic of multivesicular bodies in T lymphocytes
T-cell receptor stimulation induces the convergence of multivesicular bodies towards the microtubule- organizing centre (MTOC) and the polarization oftheMTOCtotheimmunesynapse(IS).Theseevents lead to exosome secretion at the IS. We describe herethat upon IS formation centrosomal area F-actin decreas...
| Autores: | , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/216307 |
| Acceso en línea: | http://hdl.handle.net/10261/216307 |
| Access Level: | acceso abierto |
| Palabra clave: | T lymphocytes Immune synapse Actin cytoskeleton Protein kinase C δ Centrosomes Multivesicular bodies, FMNL1 Paxillin |
| Sumario: | T-cell receptor stimulation induces the convergence of multivesicular bodies towards the microtubule- organizing centre (MTOC) and the polarization oftheMTOCtotheimmunesynapse(IS).Theseevents lead to exosome secretion at the IS. We describe herethat upon IS formation centrosomal area F-actin decreased concomitantly with MTOC polarization to the IS. PKCδ-interfered T cell clones showed a sustained level of centrosomal area F-actin associated with defective MTOC polarization. We analysed the contribution of two actin cytoskeleton-regulatory proteins, FMNL1 and paxillin, to the regulation of cortical and centrosomal F-actin networks. FMNL1βphosphorylation and F-actin reorganization at the IS were inhibited in PKCδ-interfered clones. F-actin depletion at the central region of the IS, a requirement for MTOC polarization, was associated with FMNL1βphosphorylation at its C-terminal, autoregulatory region. Interfering all FMNL1 isoforms prevented MTOC polarization;nonetheless, FMNL1βre-expres- sion restored MTOC polarization in a centrosomal area F-actin reorganization-independent manner. Moreover, PKCδ-interfered clones exhibited decreased paxillin phosphorylation at the MTOC, which suggests an alternative actin cytoskeleton regulatory pathway. Our results infer that PKCδregulates MTOC polarization and secretory traffic leading to exosome secretion in a coordinated manner by means of two distinct pathways, one involving FMNL1βregulation and controlling F-actin reorganiza- tion at the IS, and the other, comprising paxillin phosphorylation potentially controlling centrosomal area F-actin reorganization. |
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