The pH sensitivity of Aqp0 channels in tetraploid and diploid teleosts

13 pages, 7 figures, 2 tables

Detalles Bibliográficos
Autores: Chauvigné, François, Zapater, Cinta, Stavang, Jon A., Taranger, Geir L., Cerdà, Joan, Finn, Roderick N.
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/124082
Acceso en línea:http://hdl.handle.net/10261/124082
Access Level:acceso abierto
Palabra clave:Aquaporins
Water transport
Permeability lens
Cataract
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spelling The pH sensitivity of Aqp0 channels in tetraploid and diploid teleostsChauvigné, FrançoisZapater, CintaStavang, Jon A.Taranger, Geir L.Cerdà, JoanFinn, Roderick N.AquaporinsWater transportPermeability lensCataract13 pages, 7 figures, 2 tablesWater homeostasis and the structural integrity of the vertebrate lens is partially mediated by AQP0 channels. Emerging evidence indicates that external pH may be involved in channel gating. Here we show that a tetraploid teleost, the Atlantic salmon, retains 4 aqp0 genes (aqp0a1, -0a2, -0b1, and -0b2), which are highly, but not exclusively, expressed in the lens. Functional characterization reveals that, although each paralog permeates water efficiently, the permeability is respectively shifted to the neutral, alkaline, or acidic pH in Aqp0a1, -0a2, and -0b1, whereas that of Aqp0b2 is not regulated by external pH. Mutagenesis studies demonstrate that Ser, His, and His residues in the extracellular transmembrane domain of a-helix 2 facing the water pore are critical for the pH modulation of water transport. To validate these findings, we show that both zebrafish Aqp0a and -0b are functional water channels with respective pH sensitivities toward alkaline or acid pH ranges and that an N-terminal allelic variant (Ser) of Aqp0b exists that abolishes water transport in Xenopus laevis oocytes. The data suggest that the alkaline pH sensitivity is a conserved trait in teleost Aqp0 a-type channels, whereas mammalian AQP0 and some teleost Aqp0 b-type channels display an acidic pH permeation preference. © The Author(s)This work was supported by Research Council of Norway (RCN) Projects 224816/E40 and 178837/40 (to R.N.F.), and Spanish Ministry of Science and Innovation (MICINN) Grant AGL2010-15597 (to J.C.). F.C. and J.A.S. were supported by postdoctoral fellowships from the RCN (224816/E40 and 178837/40, respectively); C.Z. was supported by a predoctoral fellowship (FPI) from MICINNPeer ReviewedFederation of American Societies for Experimental BiologyMinisterio de Ciencia e Innovación (España)Research Council of NorwayConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]20152015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/124082reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1096/fj.14-267625Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1240822026-05-22T06:33:51Z
dc.title.none.fl_str_mv The pH sensitivity of Aqp0 channels in tetraploid and diploid teleosts
title The pH sensitivity of Aqp0 channels in tetraploid and diploid teleosts
spellingShingle The pH sensitivity of Aqp0 channels in tetraploid and diploid teleosts
Chauvigné, François
Aquaporins
Water transport
Permeability lens
Cataract
title_short The pH sensitivity of Aqp0 channels in tetraploid and diploid teleosts
title_full The pH sensitivity of Aqp0 channels in tetraploid and diploid teleosts
title_fullStr The pH sensitivity of Aqp0 channels in tetraploid and diploid teleosts
title_full_unstemmed The pH sensitivity of Aqp0 channels in tetraploid and diploid teleosts
title_sort The pH sensitivity of Aqp0 channels in tetraploid and diploid teleosts
dc.creator.none.fl_str_mv Chauvigné, François
Zapater, Cinta
Stavang, Jon A.
Taranger, Geir L.
Cerdà, Joan
Finn, Roderick N.
author Chauvigné, François
author_facet Chauvigné, François
Zapater, Cinta
Stavang, Jon A.
Taranger, Geir L.
Cerdà, Joan
Finn, Roderick N.
author_role author
author2 Zapater, Cinta
Stavang, Jon A.
Taranger, Geir L.
Cerdà, Joan
Finn, Roderick N.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Research Council of Norway
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Aquaporins
Water transport
Permeability lens
Cataract
topic Aquaporins
Water transport
Permeability lens
Cataract
description 13 pages, 7 figures, 2 tables
publishDate 2015
dc.date.none.fl_str_mv 2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/124082
url http://hdl.handle.net/10261/124082
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1096/fj.14-267625
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Federation of American Societies for Experimental Biology
publisher.none.fl_str_mv Federation of American Societies for Experimental Biology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869413822438047744
score 15.811543

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