Presence of viruses in wild eels Anguilla anguilla L, from the Albufera Lake (Spain)
A virological analysis was conducted on wild eels from the Albufera Lake (Spain). A total of 179 individuals at different growth stages were collected in two different surveys (2004 and 2008). Presence of anguillid herpesvirus (AngHV-1), aquabirnavirus and betanodavirus was confirmed by PCR procedur...
| Autores: | , , , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de publicación: | 2014 |
| País: | España |
| Recursos: | Universidad de Santiago de Compostela (USC) |
| Repositorio: | Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela |
| Idioma: | inglés |
| OAI Identifier: | oai:minerva.usc.gal:10347/46010 |
| Acesso em linha: | https://hdl.handle.net/10347/46010 |
| Access Level: | acceso abierto |
| Palavra-chave: | Anguilla anguilla Anguillid herpesvirus Aquabirnaviruses Betanodaviruses Wild eels 24 Ciencias de la vida |
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Presence of viruses in wild eels Anguilla anguilla L, from the Albufera Lake (Spain)Bandín Matos, IsabelSouto Pereira, SandraCutrín, J. M.López Vázquez, CarmenOlveira Hermida, José G.Esteve Sánchez, ConsueloAlcaide Moreno, ElenaPereira Dopazo, CarlosAnguilla anguillaAnguillid herpesvirusAquabirnavirusesBetanodavirusesWild eels24 Ciencias de la vidaA virological analysis was conducted on wild eels from the Albufera Lake (Spain). A total of 179 individuals at different growth stages were collected in two different surveys (2004 and 2008). Presence of anguillid herpesvirus (AngHV-1), aquabirnavirus and betanodavirus was confirmed by PCR procedures in both surveys, although the number of detections was clearly higher in 2008 (83% of the eels analysed resulted positive for virus presence). AngHV-1 was the viral agent most frequently detected, followed by aquabirnaviruses. Betanodaviruses were detected by the first time in wild eels, and although the detections were only made by nested PCR, high percentage of positives were achieved. In addition, in 2008, seven aquabirnaviruses were isolated. Phylogenetic analysis performed using partial sequences of both genomic segments of aquabirnaviruses indicated that the seven isolates could be typed as WB (genogroup I) on the basis of segment A sequences, but when segment B was used six of them clustered with C1 strain (genogroup V) and one was typed as Ab (genogroup II). These results indicate natural reassortment between different strains of aquabirnaviruses in the eels. Although betanodaviruses were not isolated in cell culture, the analysis of the sequence of the nested PCR product indicated that they clustered with SJNNV genotype. The diversity of viral agents and the high level of viral detections suggest that viral infections may play a more prominent role in the decline of the European eel than initially thoughtWileyUniversidade de Santiago de Compostela. Instituto de Acuicultura (IA)Universidade de Santiago de Compostela. Departamento de Microbioloxía e ParasitoloxíaUniversidade de Santiago de Compostela. Instituto de Investigación do Medio Acuático para Unha Saúde Global (iARCUS)20142014-05-2020142014-05-20journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10347/46010reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostelainstname:Universidad de Santiago de Compostela (USC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:minerva.usc.gal:10347/460102026-06-15T12:47:27Z |
| dc.title.none.fl_str_mv |
Presence of viruses in wild eels Anguilla anguilla L, from the Albufera Lake (Spain) |
| title |
Presence of viruses in wild eels Anguilla anguilla L, from the Albufera Lake (Spain) |
| spellingShingle |
Presence of viruses in wild eels Anguilla anguilla L, from the Albufera Lake (Spain) Bandín Matos, Isabel Anguilla anguilla Anguillid herpesvirus Aquabirnaviruses Betanodaviruses Wild eels 24 Ciencias de la vida |
| title_short |
Presence of viruses in wild eels Anguilla anguilla L, from the Albufera Lake (Spain) |
| title_full |
Presence of viruses in wild eels Anguilla anguilla L, from the Albufera Lake (Spain) |
| title_fullStr |
Presence of viruses in wild eels Anguilla anguilla L, from the Albufera Lake (Spain) |
| title_full_unstemmed |
Presence of viruses in wild eels Anguilla anguilla L, from the Albufera Lake (Spain) |
| title_sort |
Presence of viruses in wild eels Anguilla anguilla L, from the Albufera Lake (Spain) |
| dc.creator.none.fl_str_mv |
Bandín Matos, Isabel Souto Pereira, Sandra Cutrín, J. M. López Vázquez, Carmen Olveira Hermida, José G. Esteve Sánchez, Consuelo Alcaide Moreno, Elena Pereira Dopazo, Carlos |
| author |
Bandín Matos, Isabel |
| author_facet |
Bandín Matos, Isabel Souto Pereira, Sandra Cutrín, J. M. López Vázquez, Carmen Olveira Hermida, José G. Esteve Sánchez, Consuelo Alcaide Moreno, Elena Pereira Dopazo, Carlos |
| author_role |
author |
| author2 |
Souto Pereira, Sandra Cutrín, J. M. López Vázquez, Carmen Olveira Hermida, José G. Esteve Sánchez, Consuelo Alcaide Moreno, Elena Pereira Dopazo, Carlos |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade de Santiago de Compostela. Instituto de Acuicultura (IA) Universidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía Universidade de Santiago de Compostela. Instituto de Investigación do Medio Acuático para Unha Saúde Global (iARCUS) |
| dc.subject.none.fl_str_mv |
Anguilla anguilla Anguillid herpesvirus Aquabirnaviruses Betanodaviruses Wild eels 24 Ciencias de la vida |
| topic |
Anguilla anguilla Anguillid herpesvirus Aquabirnaviruses Betanodaviruses Wild eels 24 Ciencias de la vida |
| description |
A virological analysis was conducted on wild eels from the Albufera Lake (Spain). A total of 179 individuals at different growth stages were collected in two different surveys (2004 and 2008). Presence of anguillid herpesvirus (AngHV-1), aquabirnavirus and betanodavirus was confirmed by PCR procedures in both surveys, although the number of detections was clearly higher in 2008 (83% of the eels analysed resulted positive for virus presence). AngHV-1 was the viral agent most frequently detected, followed by aquabirnaviruses. Betanodaviruses were detected by the first time in wild eels, and although the detections were only made by nested PCR, high percentage of positives were achieved. In addition, in 2008, seven aquabirnaviruses were isolated. Phylogenetic analysis performed using partial sequences of both genomic segments of aquabirnaviruses indicated that the seven isolates could be typed as WB (genogroup I) on the basis of segment A sequences, but when segment B was used six of them clustered with C1 strain (genogroup V) and one was typed as Ab (genogroup II). These results indicate natural reassortment between different strains of aquabirnaviruses in the eels. Although betanodaviruses were not isolated in cell culture, the analysis of the sequence of the nested PCR product indicated that they clustered with SJNNV genotype. The diversity of viral agents and the high level of viral detections suggest that viral infections may play a more prominent role in the decline of the European eel than initially thought |
| publishDate |
2014 |
| dc.date.none.fl_str_mv |
2014 2014-05-20 2014 2014-05-20 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 AM http://purl.org/coar/version/c_ab4af688f83e57aa |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/10347/46010 |
| url |
https://hdl.handle.net/10347/46010 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Wiley |
| publisher.none.fl_str_mv |
Wiley |
| dc.source.none.fl_str_mv |
reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela instname:Universidad de Santiago de Compostela (USC) |
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Universidad de Santiago de Compostela (USC) |
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Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela |
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Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela |
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15.812429 |