Endogenous XIAP but no other members of the inhibitory apoptosis protein family modulates cerebellar granule neurons survival

Programmed cell death plays a critical role during cerebellar development. In particular, it has been shown in vivo and in vitro that developing cerebellar granule neurons (CGN) die apoptotically. Apoptosis involves a series of morphological changes and the activation of caspases. Inhibitor of apopt...

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Autores: Blancas, Sugela, Fadó, Rut|||0000-0002-3293-2342, Rodríguez Álvarez, José|||0000-0001-8582-8082, Morán, Julio
Tipo de recurso: artículo
Fecha de publicación:2014
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:171158
Acceso en línea:https://ddd.uab.cat/record/171158
https://dx.doi.org/urn:doi:10.1016/j.ijdevneu.2014.06.006
Access Level:acceso abierto
Palabra clave:Apoptotic-like death
IAPs
Caspases
Cerebellum development
Cerebellar granule neurons
Potassium deprivation
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spelling Endogenous XIAP but no other members of the inhibitory apoptosis protein family modulates cerebellar granule neurons survivalBlancas, SugelaFadó, Rut|||0000-0002-3293-2342Rodríguez Álvarez, José|||0000-0001-8582-8082Morán, JulioApoptotic-like deathIAPsCaspasesCerebellum developmentCerebellar granule neuronsPotassium deprivationProgrammed cell death plays a critical role during cerebellar development. In particular, it has been shown in vivo and in vitro that developing cerebellar granule neurons (CGN) die apoptotically. Apoptosis involves a series of morphological changes and the activation of caspases. Inhibitor of apoptosis proteins (IAPs) is implicated in negative regulation of caspase activation and apoptotic cell death. Although apoptotic death of CGN has been extensively studied, there is no information about the role of IAPs in the developing cerebellum. Here, we studied the participation of some members of IAPs in the survival of the developing rat CGN in culture and under physiological conditions. Under these conditions, we found a differential expression pattern of cIAP-1, cIAP-2, XIAP and survivin during cerebellar development in an age-dependent manner, highlighting the significant increase of XIAP levels. We also detected an interaction between XIAP and caspase 3 at postnatal day (P) 12 and 16. On the other hand, we found a significant decrease of XIAP levels in cultured CGN maintained in chronic potassium deprivation, an apoptotic condition, suggesting a possible relationship between XIAP levels and neuronal viability. Under these conditions, we also detected the interaction of XIAP with active caspase-3. The down-regulation of XIAP in CGN cultured under survival conditions (chronic potassium depolarization) induced a reduction of cell viability and an increment of apoptotic cells. These findings support the idea that IAPs could be involved in the survival of CGN and that XIAP might be critical for neuronal survival in cerebellar development and during chronic depolarization in cultured CGN through a mechanism involving caspase inhibition. 22014-01-0120142014-01-01Articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/171158https://dx.doi.org/urn:doi:10.1016/j.ijdevneu.2014.06.006reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Ciencia e Innovación https://doi.org/10.13039/501100004837 SAF2008-01904Ministerio de Sanidad y Consumo https://doi.org/10.13039/501100004587 RD06/0026/1009Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2009/SGR-1231open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1711582026-06-06T12:50:31Z
dc.title.none.fl_str_mv Endogenous XIAP but no other members of the inhibitory apoptosis protein family modulates cerebellar granule neurons survival
title Endogenous XIAP but no other members of the inhibitory apoptosis protein family modulates cerebellar granule neurons survival
spellingShingle Endogenous XIAP but no other members of the inhibitory apoptosis protein family modulates cerebellar granule neurons survival
Blancas, Sugela
Apoptotic-like death
IAPs
Caspases
Cerebellum development
Cerebellar granule neurons
Potassium deprivation
title_short Endogenous XIAP but no other members of the inhibitory apoptosis protein family modulates cerebellar granule neurons survival
title_full Endogenous XIAP but no other members of the inhibitory apoptosis protein family modulates cerebellar granule neurons survival
title_fullStr Endogenous XIAP but no other members of the inhibitory apoptosis protein family modulates cerebellar granule neurons survival
title_full_unstemmed Endogenous XIAP but no other members of the inhibitory apoptosis protein family modulates cerebellar granule neurons survival
title_sort Endogenous XIAP but no other members of the inhibitory apoptosis protein family modulates cerebellar granule neurons survival
dc.creator.none.fl_str_mv Blancas, Sugela
Fadó, Rut|||0000-0002-3293-2342
Rodríguez Álvarez, José|||0000-0001-8582-8082
Morán, Julio
author Blancas, Sugela
author_facet Blancas, Sugela
Fadó, Rut|||0000-0002-3293-2342
Rodríguez Álvarez, José|||0000-0001-8582-8082
Morán, Julio
author_role author
author2 Fadó, Rut|||0000-0002-3293-2342
Rodríguez Álvarez, José|||0000-0001-8582-8082
Morán, Julio
author2_role author
author
author
dc.subject.none.fl_str_mv Apoptotic-like death
IAPs
Caspases
Cerebellum development
Cerebellar granule neurons
Potassium deprivation
topic Apoptotic-like death
IAPs
Caspases
Cerebellum development
Cerebellar granule neurons
Potassium deprivation
description Programmed cell death plays a critical role during cerebellar development. In particular, it has been shown in vivo and in vitro that developing cerebellar granule neurons (CGN) die apoptotically. Apoptosis involves a series of morphological changes and the activation of caspases. Inhibitor of apoptosis proteins (IAPs) is implicated in negative regulation of caspase activation and apoptotic cell death. Although apoptotic death of CGN has been extensively studied, there is no information about the role of IAPs in the developing cerebellum. Here, we studied the participation of some members of IAPs in the survival of the developing rat CGN in culture and under physiological conditions. Under these conditions, we found a differential expression pattern of cIAP-1, cIAP-2, XIAP and survivin during cerebellar development in an age-dependent manner, highlighting the significant increase of XIAP levels. We also detected an interaction between XIAP and caspase 3 at postnatal day (P) 12 and 16. On the other hand, we found a significant decrease of XIAP levels in cultured CGN maintained in chronic potassium deprivation, an apoptotic condition, suggesting a possible relationship between XIAP levels and neuronal viability. Under these conditions, we also detected the interaction of XIAP with active caspase-3. The down-regulation of XIAP in CGN cultured under survival conditions (chronic potassium depolarization) induced a reduction of cell viability and an increment of apoptotic cells. These findings support the idea that IAPs could be involved in the survival of CGN and that XIAP might be critical for neuronal survival in cerebellar development and during chronic depolarization in cultured CGN through a mechanism involving caspase inhibition.
publishDate 2014
dc.date.none.fl_str_mv 2
2014-01-01
2014
2014-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/171158
https://dx.doi.org/urn:doi:10.1016/j.ijdevneu.2014.06.006
url https://ddd.uab.cat/record/171158
https://dx.doi.org/urn:doi:10.1016/j.ijdevneu.2014.06.006
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 SAF2008-01904
Ministerio de Sanidad y Consumo https://doi.org/10.13039/501100004587 RD06/0026/1009
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2009/SGR-1231
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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