A Deletion Variant of the Aspergillus fumigatus Ribotoxin Asp f 1 Induces an Attenuated Airway Inflammatory Response in a Mouse Model of Sensitization

α-Sarcin is a natural variant of Asp f 1 produced by the nonpathogenic fungus Aspergillus giganteus. Both proteins show a sequence identity of 87% and almost identical 3-dimensional structures. α-Sarcin Δ(7-22) is a deletion mutant that displays reduced immunoglobulin (Ig) E reactivity and is much l...

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Autores: Álvarez García, Elisa, Batanero Cremades, Eva, García Fernández, Rosa Ana, Villalba Díaz, María Teresa, Gavilanes, José G., Martínez Del Pozo, Álvaro
Tipo de recurso: artículo
Fecha de publicación:2010
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/41754
Acceso en línea:https://hdl.handle.net/20.500.14352/41754
Access Level:acceso abierto
Palabra clave:Aspergillus
Sarcin
Ribotoxin
Asp f 1
Allergen
Ribonuclease
Bioquímica (Biología)
Alergología
2302 Bioquímica
3207.01 Alergias
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oai_identifier_str oai:docta.ucm.es:20.500.14352/41754
network_acronym_str ES
network_name_str España
repository_id_str
spelling A Deletion Variant of the Aspergillus fumigatus Ribotoxin Asp f 1 Induces an Attenuated Airway Inflammatory Response in a Mouse Model of SensitizationÁlvarez García, ElisaBatanero Cremades, EvaGarcía Fernández, Rosa AnaVillalba Díaz, María TeresaGavilanes, José G.Martínez Del Pozo, ÁlvaroAspergillusSarcinRibotoxinAsp f 1AllergenRibonucleaseBioquímica (Biología)Alergología2302 Bioquímica3207.01 Alergiasα-Sarcin is a natural variant of Asp f 1 produced by the nonpathogenic fungus Aspergillus giganteus. Both proteins show a sequence identity of 87% and almost identical 3-dimensional structures. α-Sarcin Δ(7-22) is a deletion mutant that displays reduced immunoglobulin (Ig) E reactivity and is much less cytotoxic than wild-type proteins against human transformed cells. Objective: A murine model of sensitization to Asp f 1 was established to test the response elicited by this α-sarcin Δ(7-22) deletion mutant. Methods: BALB/c mice were treated intraperitoneally with different mixtures of recombinant wild-type Asp f 1 and/or a suspension of a commercially available A fumigatus standard extract. Mice were then intranasally challenged with Asp f 1 or α-sarcin Δ(7-22). Sera were collected for subsequent measurement of Ig levels and histological analysis of the nostrils and lungs. Results: Sensitization to Asp f 1 was successful only when the purifi ed protein was fi rst administered together with the A fumigatus suspension. The model was characterized by elevated levels of total IgE in serum and histological lesions in the lungs and nostrils. These symptoms were less severe when the deletion variant was the protein administered, thus confi rming in vivo its lower toxic character. Conclusions: An easily reproducible mouse model of A fumigatus Asp f 1 sensitization was established. This model revealed α-sarcin Δ (7-22) to be a potential candidate for immunotherapy.Esmon PublicidadUniversidad Complutense de Madrid20102010-03-0120102010-03-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/41754reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/417542026-06-02T12:44:21Z
dc.title.none.fl_str_mv A Deletion Variant of the Aspergillus fumigatus Ribotoxin Asp f 1 Induces an Attenuated Airway Inflammatory Response in a Mouse Model of Sensitization
title A Deletion Variant of the Aspergillus fumigatus Ribotoxin Asp f 1 Induces an Attenuated Airway Inflammatory Response in a Mouse Model of Sensitization
spellingShingle A Deletion Variant of the Aspergillus fumigatus Ribotoxin Asp f 1 Induces an Attenuated Airway Inflammatory Response in a Mouse Model of Sensitization
Álvarez García, Elisa
Aspergillus
Sarcin
Ribotoxin
Asp f 1
Allergen
Ribonuclease
Bioquímica (Biología)
Alergología
2302 Bioquímica
3207.01 Alergias
title_short A Deletion Variant of the Aspergillus fumigatus Ribotoxin Asp f 1 Induces an Attenuated Airway Inflammatory Response in a Mouse Model of Sensitization
title_full A Deletion Variant of the Aspergillus fumigatus Ribotoxin Asp f 1 Induces an Attenuated Airway Inflammatory Response in a Mouse Model of Sensitization
title_fullStr A Deletion Variant of the Aspergillus fumigatus Ribotoxin Asp f 1 Induces an Attenuated Airway Inflammatory Response in a Mouse Model of Sensitization
title_full_unstemmed A Deletion Variant of the Aspergillus fumigatus Ribotoxin Asp f 1 Induces an Attenuated Airway Inflammatory Response in a Mouse Model of Sensitization
title_sort A Deletion Variant of the Aspergillus fumigatus Ribotoxin Asp f 1 Induces an Attenuated Airway Inflammatory Response in a Mouse Model of Sensitization
dc.creator.none.fl_str_mv Álvarez García, Elisa
Batanero Cremades, Eva
García Fernández, Rosa Ana
Villalba Díaz, María Teresa
Gavilanes, José G.
Martínez Del Pozo, Álvaro
author Álvarez García, Elisa
author_facet Álvarez García, Elisa
Batanero Cremades, Eva
García Fernández, Rosa Ana
Villalba Díaz, María Teresa
Gavilanes, José G.
Martínez Del Pozo, Álvaro
author_role author
author2 Batanero Cremades, Eva
García Fernández, Rosa Ana
Villalba Díaz, María Teresa
Gavilanes, José G.
Martínez Del Pozo, Álvaro
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv Aspergillus
Sarcin
Ribotoxin
Asp f 1
Allergen
Ribonuclease
Bioquímica (Biología)
Alergología
2302 Bioquímica
3207.01 Alergias
topic Aspergillus
Sarcin
Ribotoxin
Asp f 1
Allergen
Ribonuclease
Bioquímica (Biología)
Alergología
2302 Bioquímica
3207.01 Alergias
description α-Sarcin is a natural variant of Asp f 1 produced by the nonpathogenic fungus Aspergillus giganteus. Both proteins show a sequence identity of 87% and almost identical 3-dimensional structures. α-Sarcin Δ(7-22) is a deletion mutant that displays reduced immunoglobulin (Ig) E reactivity and is much less cytotoxic than wild-type proteins against human transformed cells. Objective: A murine model of sensitization to Asp f 1 was established to test the response elicited by this α-sarcin Δ(7-22) deletion mutant. Methods: BALB/c mice were treated intraperitoneally with different mixtures of recombinant wild-type Asp f 1 and/or a suspension of a commercially available A fumigatus standard extract. Mice were then intranasally challenged with Asp f 1 or α-sarcin Δ(7-22). Sera were collected for subsequent measurement of Ig levels and histological analysis of the nostrils and lungs. Results: Sensitization to Asp f 1 was successful only when the purifi ed protein was fi rst administered together with the A fumigatus suspension. The model was characterized by elevated levels of total IgE in serum and histological lesions in the lungs and nostrils. These symptoms were less severe when the deletion variant was the protein administered, thus confi rming in vivo its lower toxic character. Conclusions: An easily reproducible mouse model of A fumigatus Asp f 1 sensitization was established. This model revealed α-sarcin Δ (7-22) to be a potential candidate for immunotherapy.
publishDate 2010
dc.date.none.fl_str_mv 2010
2010-03-01
2010
2010-03-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/41754
url https://hdl.handle.net/20.500.14352/41754
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Esmon Publicidad
publisher.none.fl_str_mv Esmon Publicidad
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869413783353425920
score 15,300724

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