Validation of an automated extraction procedure for amino acids and acylcarnitines for use with tandem mass spectrometry for newborn screening

3 Tablas.- 1 Figura.- Datos suplementarios disponibles en la página web del editor.

Detalles Bibliográficos
Autores: Gaugler, Stefan Christian, Rykl, Jana, Cebolla, Vicente L.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/180034
Acceso en línea:http://researchopenworld.com/wp-content/uploads/2019/01/EDMJ-2019-102-Gaugler-Stefan-Switzerland.pdf
http://hdl.handle.net/10261/180034
Access Level:acceso abierto
Palabra clave:Dried blood spot
Newborn
Screening
Amino acid
Carnitine
Automation
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spelling Validation of an automated extraction procedure for amino acids and acylcarnitines for use with tandem mass spectrometry for newborn screeningGaugler, Stefan ChristianRykl, JanaCebolla, Vicente L.Dried blood spotNewbornScreeningAmino acidCarnitineAutomation3 Tablas.- 1 Figura.- Datos suplementarios disponibles en la página web del editor.A certified reagent kit for newborn screening was transferred on a fully automated dried blood spot platform. The dried blood spot cards are directly eluted and the extract is online guided to tandem mass spectrometry instrument, where the amino acid and acyl carnitine panel is detected. The method takes 2 minutes per sample and requires no human interaction for up to 500 samples. The method is fully standardized through the automation and the usage of only certified consumables and reference material. The manual reagent kit was first modified to fit the automated platform, secondly validated and third, successfully transferred into a routine newborn screening laboratory.Peer reviewedResearch Open WorldGaugler, Stefan Christian [0000-0001-9059-0291]Cebolla, Vicente L. [0000-0002-9786-9217]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://researchopenworld.com/wp-content/uploads/2019/01/EDMJ-2019-102-Gaugler-Stefan-Switzerland.pdfhttp://hdl.handle.net/10261/180034reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://researchopenworld.com/wp-content/uploads/2019/01/EDMJ-2019-102-Gaugler-Stefan-Switzerland.pdfSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1800342026-05-22T06:33:51Z
dc.title.none.fl_str_mv Validation of an automated extraction procedure for amino acids and acylcarnitines for use with tandem mass spectrometry for newborn screening
title Validation of an automated extraction procedure for amino acids and acylcarnitines for use with tandem mass spectrometry for newborn screening
spellingShingle Validation of an automated extraction procedure for amino acids and acylcarnitines for use with tandem mass spectrometry for newborn screening
Gaugler, Stefan Christian
Dried blood spot
Newborn
Screening
Amino acid
Carnitine
Automation
title_short Validation of an automated extraction procedure for amino acids and acylcarnitines for use with tandem mass spectrometry for newborn screening
title_full Validation of an automated extraction procedure for amino acids and acylcarnitines for use with tandem mass spectrometry for newborn screening
title_fullStr Validation of an automated extraction procedure for amino acids and acylcarnitines for use with tandem mass spectrometry for newborn screening
title_full_unstemmed Validation of an automated extraction procedure for amino acids and acylcarnitines for use with tandem mass spectrometry for newborn screening
title_sort Validation of an automated extraction procedure for amino acids and acylcarnitines for use with tandem mass spectrometry for newborn screening
dc.creator.none.fl_str_mv Gaugler, Stefan Christian
Rykl, Jana
Cebolla, Vicente L.
author Gaugler, Stefan Christian
author_facet Gaugler, Stefan Christian
Rykl, Jana
Cebolla, Vicente L.
author_role author
author2 Rykl, Jana
Cebolla, Vicente L.
author2_role author
author
dc.contributor.none.fl_str_mv Gaugler, Stefan Christian [0000-0001-9059-0291]
Cebolla, Vicente L. [0000-0002-9786-9217]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Dried blood spot
Newborn
Screening
Amino acid
Carnitine
Automation
topic Dried blood spot
Newborn
Screening
Amino acid
Carnitine
Automation
description 3 Tablas.- 1 Figura.- Datos suplementarios disponibles en la página web del editor.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://researchopenworld.com/wp-content/uploads/2019/01/EDMJ-2019-102-Gaugler-Stefan-Switzerland.pdf
http://hdl.handle.net/10261/180034
url http://researchopenworld.com/wp-content/uploads/2019/01/EDMJ-2019-102-Gaugler-Stefan-Switzerland.pdf
http://hdl.handle.net/10261/180034
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://researchopenworld.com/wp-content/uploads/2019/01/EDMJ-2019-102-Gaugler-Stefan-Switzerland.pdf
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Research Open World
publisher.none.fl_str_mv Research Open World
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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