Ovothiol ensures the correct developmental programme of the sea urchin Paracentrotus lividus embryo
Ovothiols are π-methyl-5-thiohistidines produced in great amounts in sea urchin eggs, where they can act as protective agents against the oxidative burst at fertilization and environmental stressors during development. Here we examined the biological relevance of ovothiol during the embryogenesis of...
| Autores: | , , , , , , , |
|---|---|
| Tipo de documento: | artigo |
| Data de publicação: | 2022 |
| País: | España |
| Recursos: | Universitat Autònoma de Barcelona |
| Repositório: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglês |
| OAI Identifier: | oai:ddd.uab.cat:274300 |
| Acesso em linha: | https://ddd.uab.cat/record/274300 https://dx.doi.org/urn:doi:10.1098/rsob.210262 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Ovothiol Oxidative stress Cell proliferation Sea urchin Embryonic development Marine organisms |
| id |
ES_94edd953b3a3d82da5df8bdb064a8ce4 |
|---|---|
| oai_identifier_str |
oai:ddd.uab.cat:274300 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| spelling |
Ovothiol ensures the correct developmental programme of the sea urchin Paracentrotus lividus embryoMilito, Alfonsina|||0000-0002-5541-6113Cocurullo, Maria|||0000-0002-7729-3281Columbro, Alfredo|||0000-0002-0303-1115Nonnis, Simona|||0000-0002-3453-7282Tedeschi, GabriellaCastellano, Immacolata|||0000-0002-4274-6738Arnone, Maria Ina|||0000-0002-9012-7624Palumbo, Anna|||0000-0002-5972-5589OvothiolOxidative stressCell proliferationSea urchinEmbryonic developmentMarine organismsOvothiols are π-methyl-5-thiohistidines produced in great amounts in sea urchin eggs, where they can act as protective agents against the oxidative burst at fertilization and environmental stressors during development. Here we examined the biological relevance of ovothiol during the embryogenesis of the sea urchin Paracentrotus lividus by assessing the localization of the key biosynthetic enzyme OvoA, both at transcript and protein level, and perturbing its protein translation by morpholino antisense oligonucleotide-mediated knockdown experiments. In addition, we explored the possible involvement of ovothiol in the inflammatory response by assessing ovoA gene expression and protein localization following exposure to bacterial lipopolysaccharide. The results of the present study suggest that ovothiol may be a key regulator of cell proliferation in early developing embryos. Moreover, the localization of OvoA in key larval cells and tissues, in control and inflammatory conditions, suggests that ovothiol may ensure larval skeleton formation and mediate inflammatory processes triggered by bacterial infection. This work significantly contributes to the understanding of the biological function of ovothiols in marine organisms, and may provide new inspiration for the identification of the biological activities of ovothiols in humans, considering the pharmacological potential of these molecules. 22022-01-0120222022-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/274300https://dx.doi.org/urn:doi:10.1098/rsob.210262reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2743002026-06-06T12:50:31Z |
| dc.title.none.fl_str_mv |
Ovothiol ensures the correct developmental programme of the sea urchin Paracentrotus lividus embryo |
| title |
Ovothiol ensures the correct developmental programme of the sea urchin Paracentrotus lividus embryo |
| spellingShingle |
Ovothiol ensures the correct developmental programme of the sea urchin Paracentrotus lividus embryo Milito, Alfonsina|||0000-0002-5541-6113 Ovothiol Oxidative stress Cell proliferation Sea urchin Embryonic development Marine organisms |
| title_short |
Ovothiol ensures the correct developmental programme of the sea urchin Paracentrotus lividus embryo |
| title_full |
Ovothiol ensures the correct developmental programme of the sea urchin Paracentrotus lividus embryo |
| title_fullStr |
Ovothiol ensures the correct developmental programme of the sea urchin Paracentrotus lividus embryo |
| title_full_unstemmed |
Ovothiol ensures the correct developmental programme of the sea urchin Paracentrotus lividus embryo |
| title_sort |
Ovothiol ensures the correct developmental programme of the sea urchin Paracentrotus lividus embryo |
| dc.creator.none.fl_str_mv |
Milito, Alfonsina|||0000-0002-5541-6113 Cocurullo, Maria|||0000-0002-7729-3281 Columbro, Alfredo|||0000-0002-0303-1115 Nonnis, Simona|||0000-0002-3453-7282 Tedeschi, Gabriella Castellano, Immacolata|||0000-0002-4274-6738 Arnone, Maria Ina|||0000-0002-9012-7624 Palumbo, Anna|||0000-0002-5972-5589 |
| author |
Milito, Alfonsina|||0000-0002-5541-6113 |
| author_facet |
Milito, Alfonsina|||0000-0002-5541-6113 Cocurullo, Maria|||0000-0002-7729-3281 Columbro, Alfredo|||0000-0002-0303-1115 Nonnis, Simona|||0000-0002-3453-7282 Tedeschi, Gabriella Castellano, Immacolata|||0000-0002-4274-6738 Arnone, Maria Ina|||0000-0002-9012-7624 Palumbo, Anna|||0000-0002-5972-5589 |
| author_role |
author |
| author2 |
Cocurullo, Maria|||0000-0002-7729-3281 Columbro, Alfredo|||0000-0002-0303-1115 Nonnis, Simona|||0000-0002-3453-7282 Tedeschi, Gabriella Castellano, Immacolata|||0000-0002-4274-6738 Arnone, Maria Ina|||0000-0002-9012-7624 Palumbo, Anna|||0000-0002-5972-5589 |
| author2_role |
author author author author author author author |
| dc.subject.none.fl_str_mv |
Ovothiol Oxidative stress Cell proliferation Sea urchin Embryonic development Marine organisms |
| topic |
Ovothiol Oxidative stress Cell proliferation Sea urchin Embryonic development Marine organisms |
| description |
Ovothiols are π-methyl-5-thiohistidines produced in great amounts in sea urchin eggs, where they can act as protective agents against the oxidative burst at fertilization and environmental stressors during development. Here we examined the biological relevance of ovothiol during the embryogenesis of the sea urchin Paracentrotus lividus by assessing the localization of the key biosynthetic enzyme OvoA, both at transcript and protein level, and perturbing its protein translation by morpholino antisense oligonucleotide-mediated knockdown experiments. In addition, we explored the possible involvement of ovothiol in the inflammatory response by assessing ovoA gene expression and protein localization following exposure to bacterial lipopolysaccharide. The results of the present study suggest that ovothiol may be a key regulator of cell proliferation in early developing embryos. Moreover, the localization of OvoA in key larval cells and tissues, in control and inflammatory conditions, suggests that ovothiol may ensure larval skeleton formation and mediate inflammatory processes triggered by bacterial infection. This work significantly contributes to the understanding of the biological function of ovothiols in marine organisms, and may provide new inspiration for the identification of the biological activities of ovothiols in humans, considering the pharmacological potential of these molecules. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2 2022-01-01 2022 2022-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://ddd.uab.cat/record/274300 https://dx.doi.org/urn:doi:10.1098/rsob.210262 |
| url |
https://ddd.uab.cat/record/274300 https://dx.doi.org/urn:doi:10.1098/rsob.210262 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.source.none.fl_str_mv |
reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona |
| instname_str |
Universitat Autònoma de Barcelona |
| reponame_str |
Dipòsit Digital de Documents de la UAB |
| collection |
Dipòsit Digital de Documents de la UAB |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869413734020022272 |
| score |
15.301603 |