Cloning and expression of porcine CD163 Its use for characterization of monoclonal antibodies to porcine CD163 and development of an ELISA to measure soluble CD163 in biological fluids

CD163 is a member of the family of proteins with scavenger receptor cysteine-rich domains, whose expression is restricted to monocytes and macrophages. It functions as a receptor for haemoglobin/haptoglobin complexes and it has also been implicated in the regulation of inflammatory processes. Treatm...

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Autores: Pérez, Carlos, Ortuño, E., Gómez, Nuria, García-Briones, M. M., Álvarez, Belén, Martínez De la Riva, Paloma, Alonso, Frank, Revilla Calvo, Concepción, Domínguez, Javier, Ezquerra Martínez, Ángel
Tipo de recurso: artículo
Fecha de publicación:2008
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/291573
Acceso en línea:http://hdl.handle.net/10261/291573
Access Level:acceso abierto
Palabra clave:Macrophage
SRCR family
Swine
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spelling Cloning and expression of porcine CD163 Its use for characterization of monoclonal antibodies to porcine CD163 and development of an ELISA to measure soluble CD163 in biological fluidsPérez, CarlosOrtuño, E.Gómez, NuriaGarcía-Briones, M. M.Álvarez, BelénMartínez De la Riva, PalomaAlonso, FrankRevilla Calvo, ConcepciónDomínguez, JavierEzquerra Martínez, ÁngelMacrophageSRCR familySwineCD163 is a member of the family of proteins with scavenger receptor cysteine-rich domains, whose expression is restricted to monocytes and macrophages. It functions as a receptor for haemoglobin/haptoglobin complexes and it has also been implicated in the regulation of inflammatory processes. Treatment of monocytes/macrophages with phorbol esters, LPS or cross-linking of Fc gamma receptors, causes shedding of CD163 from cell surface by a protease-dependent mechanism. The level of soluble CD163 (sCD163) in serum and other body fluids has been considered a useful marker of macrophage activation. In addition, porcine CD163 has been shown to play a role in the infection of monocytes/macrophages by the African swine fever virus. The porcine CD163 cDNA has been cloned, and expressed in transfected CHO cells. Clones of CHO cells stably expressing porcine CD163 have been used for the characterization of three new mAbs against porcine CD163. Using two of these mAbs, that bind to different epitopes on CD163 molecule, a sandwich enzyme-linked immunoassay (ELISA) has been developed to measure levels of sCD163 in porcine sera and biological fluids. The assay was calibrated using lysates of CD163 transfectants, showing a sensitivity of 105 cells mL-1, that allowed to detect sCD163 in sera and in culture supernatants of activated alveolar macrophages. This assay can be a useful method of monitoring the degree of activation of macrophages in a variety of inflammatory and infectious diseases of swine.Peer reviewedCSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)Martínez De la Riva, Paloma [0000-0003-3097-022X]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232008info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/291573reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésDepartamento de BiotecnologíaSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2915732026-05-22T06:33:51Z
dc.title.none.fl_str_mv Cloning and expression of porcine CD163 Its use for characterization of monoclonal antibodies to porcine CD163 and development of an ELISA to measure soluble CD163 in biological fluids
title Cloning and expression of porcine CD163 Its use for characterization of monoclonal antibodies to porcine CD163 and development of an ELISA to measure soluble CD163 in biological fluids
spellingShingle Cloning and expression of porcine CD163 Its use for characterization of monoclonal antibodies to porcine CD163 and development of an ELISA to measure soluble CD163 in biological fluids
Pérez, Carlos
Macrophage
SRCR family
Swine
title_short Cloning and expression of porcine CD163 Its use for characterization of monoclonal antibodies to porcine CD163 and development of an ELISA to measure soluble CD163 in biological fluids
title_full Cloning and expression of porcine CD163 Its use for characterization of monoclonal antibodies to porcine CD163 and development of an ELISA to measure soluble CD163 in biological fluids
title_fullStr Cloning and expression of porcine CD163 Its use for characterization of monoclonal antibodies to porcine CD163 and development of an ELISA to measure soluble CD163 in biological fluids
title_full_unstemmed Cloning and expression of porcine CD163 Its use for characterization of monoclonal antibodies to porcine CD163 and development of an ELISA to measure soluble CD163 in biological fluids
title_sort Cloning and expression of porcine CD163 Its use for characterization of monoclonal antibodies to porcine CD163 and development of an ELISA to measure soluble CD163 in biological fluids
dc.creator.none.fl_str_mv Pérez, Carlos
Ortuño, E.
Gómez, Nuria
García-Briones, M. M.
Álvarez, Belén
Martínez De la Riva, Paloma
Alonso, Frank
Revilla Calvo, Concepción
Domínguez, Javier
Ezquerra Martínez, Ángel
author Pérez, Carlos
author_facet Pérez, Carlos
Ortuño, E.
Gómez, Nuria
García-Briones, M. M.
Álvarez, Belén
Martínez De la Riva, Paloma
Alonso, Frank
Revilla Calvo, Concepción
Domínguez, Javier
Ezquerra Martínez, Ángel
author_role author
author2 Ortuño, E.
Gómez, Nuria
García-Briones, M. M.
Álvarez, Belén
Martínez De la Riva, Paloma
Alonso, Frank
Revilla Calvo, Concepción
Domínguez, Javier
Ezquerra Martínez, Ángel
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Martínez De la Riva, Paloma [0000-0003-3097-022X]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Macrophage
SRCR family
Swine
topic Macrophage
SRCR family
Swine
description CD163 is a member of the family of proteins with scavenger receptor cysteine-rich domains, whose expression is restricted to monocytes and macrophages. It functions as a receptor for haemoglobin/haptoglobin complexes and it has also been implicated in the regulation of inflammatory processes. Treatment of monocytes/macrophages with phorbol esters, LPS or cross-linking of Fc gamma receptors, causes shedding of CD163 from cell surface by a protease-dependent mechanism. The level of soluble CD163 (sCD163) in serum and other body fluids has been considered a useful marker of macrophage activation. In addition, porcine CD163 has been shown to play a role in the infection of monocytes/macrophages by the African swine fever virus. The porcine CD163 cDNA has been cloned, and expressed in transfected CHO cells. Clones of CHO cells stably expressing porcine CD163 have been used for the characterization of three new mAbs against porcine CD163. Using two of these mAbs, that bind to different epitopes on CD163 molecule, a sandwich enzyme-linked immunoassay (ELISA) has been developed to measure levels of sCD163 in porcine sera and biological fluids. The assay was calibrated using lysates of CD163 transfectants, showing a sensitivity of 105 cells mL-1, that allowed to detect sCD163 in sera and in culture supernatants of activated alveolar macrophages. This assay can be a useful method of monitoring the degree of activation of macrophages in a variety of inflammatory and infectious diseases of swine.
publishDate 2008
dc.date.none.fl_str_mv 2008
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/291573
url http://hdl.handle.net/10261/291573
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Departamento de Biotecnología
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
publisher.none.fl_str_mv CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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