Dissimilar Effects of Selenite and Selenium Nanoparticles on Skeletal Muscle Development Unrelated to GPx1 Activity During Adolescence in Rats

Background/Objectives: During adolescence, the critical growth period, the antioxidant selenium (Se), either as sodium selenite or selenium nanoparticles (SeNPs), has shown contrasting effects on adipose tissue (AT) in rats, due to its role in insulin signaling. Since skeletal muscle (SKM) is also a...

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Autores: Nogales Bueno, Fátima, Pajuelo Domínguez, Eloísa, Gallego López, María del Carmen, Romero Herrera, Inés, Merchán Ignacio, Francisco, Carreras Sánchez, Olimpia, Ojeda Murillo, María Luisa
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Recursos:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/173902
Acesso em linha:https://hdl.handle.net/11441/173902
https://doi.org/10.3390/nu17111841
Access Level:acceso abierto
Palavra-chave:Selenite
Nanoparticles
Skeletal muscle
Glutathione peroxidase
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spelling Dissimilar Effects of Selenite and Selenium Nanoparticles on Skeletal Muscle Development Unrelated to GPx1 Activity During Adolescence in RatsNogales Bueno, FátimaPajuelo Domínguez, EloísaGallego López, María del CarmenRomero Herrera, InésMerchán Ignacio, FranciscoCarreras Sánchez, OlimpiaOjeda Murillo, María LuisaSeleniteNanoparticlesSkeletal muscleGlutathione peroxidaseBackground/Objectives: During adolescence, the critical growth period, the antioxidant selenium (Se), either as sodium selenite or selenium nanoparticles (SeNPs), has shown contrasting effects on adipose tissue (AT) in rats, due to its role in insulin signaling. Since skeletal muscle (SKM) is also a key insulin-target tissue, this study aimed to assess whether a similar effect occurs in this tissue. Methods: Three groups of male adolescent rats (n = 18) were used: control (C), selenite supplemented (S), and SeNPs supplemented (NS). Low doses of Se were administered via drinking water in both supplemented groups. AT was utilized for transcriptomic analyses, while SKM was analyzed for oxidative balance, insulin-induced anabolic effects, and proteolysis. Myokine levels in serum were also determined. Results: SeNPs administration decreased SKM mass and protein content, increased serum creatinine, and decreased insulin levels, indicating impaired SKM development. Both supplemented groups upregulated genes related to creatine metabolism and muscle contraction. However, only the NS group showed upregulation of genes associated with glycogenolysis and glycolysis. Despite unchanged GPx1 expression, NS rats presented lower oxidation and insulin–pmTOR activation, and higher expression of proteins related to proteolysis (pAMPK, SIRT1, ULK1, FOXO3a, and MaFbx) and a myokine profile compatible to muscle atrophy, fatty acid oxidation, and impaired myoblast proliferation. Ultimately, the selenite group impaired SKM catabolism mainly by increasing insulin–pmTOR activation. Conclusions: Once again, the form of Se administration exerts opposing effects on metabolism tissues, suggests a potential therapeutic role for selenite in disorders that compromise muscle growth, such as muscular dystrophies, cachexia, or sarcopenia.Multidisciplinary Digital Publishing Institute (MDPI)FisiologíaMicrobiología y ParasitologíaJunta de AndalucíaMinisterio de Ciencia, Innovación y Universidades (MICIU). España2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/173902https://doi.org/10.3390/nu17111841reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésNutrients, 17 (11), 1841.US-1380878CTS-193PID2019-109371GB-I00https://doi.org/10.3390/nu17111841info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1739022026-06-17T12:51:07Z
dc.title.none.fl_str_mv Dissimilar Effects of Selenite and Selenium Nanoparticles on Skeletal Muscle Development Unrelated to GPx1 Activity During Adolescence in Rats
title Dissimilar Effects of Selenite and Selenium Nanoparticles on Skeletal Muscle Development Unrelated to GPx1 Activity During Adolescence in Rats
spellingShingle Dissimilar Effects of Selenite and Selenium Nanoparticles on Skeletal Muscle Development Unrelated to GPx1 Activity During Adolescence in Rats
Nogales Bueno, Fátima
Selenite
Nanoparticles
Skeletal muscle
Glutathione peroxidase
title_short Dissimilar Effects of Selenite and Selenium Nanoparticles on Skeletal Muscle Development Unrelated to GPx1 Activity During Adolescence in Rats
title_full Dissimilar Effects of Selenite and Selenium Nanoparticles on Skeletal Muscle Development Unrelated to GPx1 Activity During Adolescence in Rats
title_fullStr Dissimilar Effects of Selenite and Selenium Nanoparticles on Skeletal Muscle Development Unrelated to GPx1 Activity During Adolescence in Rats
title_full_unstemmed Dissimilar Effects of Selenite and Selenium Nanoparticles on Skeletal Muscle Development Unrelated to GPx1 Activity During Adolescence in Rats
title_sort Dissimilar Effects of Selenite and Selenium Nanoparticles on Skeletal Muscle Development Unrelated to GPx1 Activity During Adolescence in Rats
dc.creator.none.fl_str_mv Nogales Bueno, Fátima
Pajuelo Domínguez, Eloísa
Gallego López, María del Carmen
Romero Herrera, Inés
Merchán Ignacio, Francisco
Carreras Sánchez, Olimpia
Ojeda Murillo, María Luisa
author Nogales Bueno, Fátima
author_facet Nogales Bueno, Fátima
Pajuelo Domínguez, Eloísa
Gallego López, María del Carmen
Romero Herrera, Inés
Merchán Ignacio, Francisco
Carreras Sánchez, Olimpia
Ojeda Murillo, María Luisa
author_role author
author2 Pajuelo Domínguez, Eloísa
Gallego López, María del Carmen
Romero Herrera, Inés
Merchán Ignacio, Francisco
Carreras Sánchez, Olimpia
Ojeda Murillo, María Luisa
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fisiología
Microbiología y Parasitología
Junta de Andalucía
Ministerio de Ciencia, Innovación y Universidades (MICIU). España
dc.subject.none.fl_str_mv Selenite
Nanoparticles
Skeletal muscle
Glutathione peroxidase
topic Selenite
Nanoparticles
Skeletal muscle
Glutathione peroxidase
description Background/Objectives: During adolescence, the critical growth period, the antioxidant selenium (Se), either as sodium selenite or selenium nanoparticles (SeNPs), has shown contrasting effects on adipose tissue (AT) in rats, due to its role in insulin signaling. Since skeletal muscle (SKM) is also a key insulin-target tissue, this study aimed to assess whether a similar effect occurs in this tissue. Methods: Three groups of male adolescent rats (n = 18) were used: control (C), selenite supplemented (S), and SeNPs supplemented (NS). Low doses of Se were administered via drinking water in both supplemented groups. AT was utilized for transcriptomic analyses, while SKM was analyzed for oxidative balance, insulin-induced anabolic effects, and proteolysis. Myokine levels in serum were also determined. Results: SeNPs administration decreased SKM mass and protein content, increased serum creatinine, and decreased insulin levels, indicating impaired SKM development. Both supplemented groups upregulated genes related to creatine metabolism and muscle contraction. However, only the NS group showed upregulation of genes associated with glycogenolysis and glycolysis. Despite unchanged GPx1 expression, NS rats presented lower oxidation and insulin–pmTOR activation, and higher expression of proteins related to proteolysis (pAMPK, SIRT1, ULK1, FOXO3a, and MaFbx) and a myokine profile compatible to muscle atrophy, fatty acid oxidation, and impaired myoblast proliferation. Ultimately, the selenite group impaired SKM catabolism mainly by increasing insulin–pmTOR activation. Conclusions: Once again, the form of Se administration exerts opposing effects on metabolism tissues, suggests a potential therapeutic role for selenite in disorders that compromise muscle growth, such as muscular dystrophies, cachexia, or sarcopenia.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/173902
https://doi.org/10.3390/nu17111841
url https://hdl.handle.net/11441/173902
https://doi.org/10.3390/nu17111841
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Nutrients, 17 (11), 1841.
US-1380878
CTS-193
PID2019-109371GB-I00
https://doi.org/10.3390/nu17111841
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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