TrkB and TrkC signaling are required for maturation and synaptogenesis of hippocampal connections
Recent studies have suggested a role for neurotrophins in the growth and refinement of neural connections, in dendritic growth, and in activity-dependent adult plasticity. To unravel the role of endogenous neurotrophins in the development of neural connections in the CNS, we studied the ontogeny of...
| Autores: | , , , , , , , , , , |
|---|---|
| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 1998 |
| País: | España |
| Recursos: | Universidad de Barcelona |
| Repositorio: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/25564 |
| Acesso em linha: | https://hdl.handle.net/2445/25564 |
| Access Level: | acceso abierto |
| Palavra-chave: | Hipocamp (Cervell) Proteïnes quinases Sinapsi Hippocampus (Brain) Protein kinases Synapses |
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TrkB and TrkC signaling are required for maturation and synaptogenesis of hippocampal connectionsMartínez García, AlbertAlcántara Horrillo, SoledadBorrell Franco, VíctorRío Fernández, José Antonio delBlasi Cabús, JoanOtal Agudo, RaquelCampos Martínez, NarcisoBoronat i Margosa, AlbertBarbacid, MarianoSilos-Santiago, InmaculadaSoriano García, EduardoHipocamp (Cervell)Proteïnes quinasesSinapsiHippocampus (Brain)Protein kinasesSynapsesRecent studies have suggested a role for neurotrophins in the growth and refinement of neural connections, in dendritic growth, and in activity-dependent adult plasticity. To unravel the role of endogenous neurotrophins in the development of neural connections in the CNS, we studied the ontogeny of hippocampal afferents intrkB (¿/¿) and trkC (¿/¿) mice. Injections of lipophilic tracers in the entorhinal cortex and hippocampus of newborn mutant mice showed that the ingrowth of entorhinal and commissural/associational afferents to the hippocampus was not affected by these mutations. Similarly, injections of biocytin in postnatal mutant mice (P10¿P16) did not reveal major differences in the topographic patterns of hippocampal connections. In contrast, quantification of biocytin-filled axons showed that commissural and entorhinal afferents have a reduced number of axon collaterals (21¿49%) and decreased densities of axonal varicosities (8¿17%) in both trkB (¿/¿) and trkC (¿/¿) mice. In addition, electron microscopic analyses showed thattrkB (¿/¿) and trkC (¿/¿) mice have lower densities of synaptic contacts and important structural alterations of presynaptic boutons, such as decreased density of synaptic vesicles. Finally, immunocytochemical studies revealed a reduced expression of the synaptic-associated proteins responsible for synaptic vesicle exocytosis and neurotransmitter release (v-SNAREs and t-SNAREs), especially in trkB (¿/¿) mice. We conclude that neither trkB nor trkC genes are essential for the ingrowth or layer-specific targeting of hippocampal connections, although the lack of these receptors results in reduced axonal arborization and synaptic density, which indicates a role for TrkB and TrkC receptors in the developmental regulation of synaptic inputs in the CNS in vivo. The data also suggest that the genes encoding for synaptic proteins may be targets of TrkB and TrkC signaling pathways.Society for Neuroscience1998info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/25564Articles publicats en revistes (Patologia i Terapèutica Experimental)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://www.jneurosci.org/content/18/18/7336The Journal of Neuroscience, 1998, vol. 18, num. 18, p. 7336-7350(c) Society for Neuroscience, 1998info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/255642026-05-27T06:46:51Z |
| dc.title.none.fl_str_mv |
TrkB and TrkC signaling are required for maturation and synaptogenesis of hippocampal connections |
| title |
TrkB and TrkC signaling are required for maturation and synaptogenesis of hippocampal connections |
| spellingShingle |
TrkB and TrkC signaling are required for maturation and synaptogenesis of hippocampal connections Martínez García, Albert Hipocamp (Cervell) Proteïnes quinases Sinapsi Hippocampus (Brain) Protein kinases Synapses |
| title_short |
TrkB and TrkC signaling are required for maturation and synaptogenesis of hippocampal connections |
| title_full |
TrkB and TrkC signaling are required for maturation and synaptogenesis of hippocampal connections |
| title_fullStr |
TrkB and TrkC signaling are required for maturation and synaptogenesis of hippocampal connections |
| title_full_unstemmed |
TrkB and TrkC signaling are required for maturation and synaptogenesis of hippocampal connections |
| title_sort |
TrkB and TrkC signaling are required for maturation and synaptogenesis of hippocampal connections |
| dc.creator.none.fl_str_mv |
Martínez García, Albert Alcántara Horrillo, Soledad Borrell Franco, Víctor Río Fernández, José Antonio del Blasi Cabús, Joan Otal Agudo, Raquel Campos Martínez, Narciso Boronat i Margosa, Albert Barbacid, Mariano Silos-Santiago, Inmaculada Soriano García, Eduardo |
| author |
Martínez García, Albert |
| author_facet |
Martínez García, Albert Alcántara Horrillo, Soledad Borrell Franco, Víctor Río Fernández, José Antonio del Blasi Cabús, Joan Otal Agudo, Raquel Campos Martínez, Narciso Boronat i Margosa, Albert Barbacid, Mariano Silos-Santiago, Inmaculada Soriano García, Eduardo |
| author_role |
author |
| author2 |
Alcántara Horrillo, Soledad Borrell Franco, Víctor Río Fernández, José Antonio del Blasi Cabús, Joan Otal Agudo, Raquel Campos Martínez, Narciso Boronat i Margosa, Albert Barbacid, Mariano Silos-Santiago, Inmaculada Soriano García, Eduardo |
| author2_role |
author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Hipocamp (Cervell) Proteïnes quinases Sinapsi Hippocampus (Brain) Protein kinases Synapses |
| topic |
Hipocamp (Cervell) Proteïnes quinases Sinapsi Hippocampus (Brain) Protein kinases Synapses |
| description |
Recent studies have suggested a role for neurotrophins in the growth and refinement of neural connections, in dendritic growth, and in activity-dependent adult plasticity. To unravel the role of endogenous neurotrophins in the development of neural connections in the CNS, we studied the ontogeny of hippocampal afferents intrkB (¿/¿) and trkC (¿/¿) mice. Injections of lipophilic tracers in the entorhinal cortex and hippocampus of newborn mutant mice showed that the ingrowth of entorhinal and commissural/associational afferents to the hippocampus was not affected by these mutations. Similarly, injections of biocytin in postnatal mutant mice (P10¿P16) did not reveal major differences in the topographic patterns of hippocampal connections. In contrast, quantification of biocytin-filled axons showed that commissural and entorhinal afferents have a reduced number of axon collaterals (21¿49%) and decreased densities of axonal varicosities (8¿17%) in both trkB (¿/¿) and trkC (¿/¿) mice. In addition, electron microscopic analyses showed thattrkB (¿/¿) and trkC (¿/¿) mice have lower densities of synaptic contacts and important structural alterations of presynaptic boutons, such as decreased density of synaptic vesicles. Finally, immunocytochemical studies revealed a reduced expression of the synaptic-associated proteins responsible for synaptic vesicle exocytosis and neurotransmitter release (v-SNAREs and t-SNAREs), especially in trkB (¿/¿) mice. We conclude that neither trkB nor trkC genes are essential for the ingrowth or layer-specific targeting of hippocampal connections, although the lack of these receptors results in reduced axonal arborization and synaptic density, which indicates a role for TrkB and TrkC receptors in the developmental regulation of synaptic inputs in the CNS in vivo. The data also suggest that the genes encoding for synaptic proteins may be targets of TrkB and TrkC signaling pathways. |
| publishDate |
1998 |
| dc.date.none.fl_str_mv |
1998 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2445/25564 |
| url |
https://hdl.handle.net/2445/25564 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Reproducció del document publicat a: http://www.jneurosci.org/content/18/18/7336 The Journal of Neuroscience, 1998, vol. 18, num. 18, p. 7336-7350 |
| dc.rights.none.fl_str_mv |
(c) Society for Neuroscience, 1998 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
(c) Society for Neuroscience, 1998 |
| eu_rights_str_mv |
openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Society for Neuroscience |
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Society for Neuroscience |
| dc.source.none.fl_str_mv |
Articles publicats en revistes (Patologia i Terapèutica Experimental) reponame:Dipòsit Digital de la UB instname:Universidad de Barcelona |
| instname_str |
Universidad de Barcelona |
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Dipòsit Digital de la UB |
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Dipòsit Digital de la UB |
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15,300724 |