Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS/dsRNA-loaded nanoliposomes

Herein we report the use of immunostimulant-loaded nanoliposomes (called NLcliposomes) as a strategy to protect fish against bacterial and/or viral infections. This work entailed developing a method for in vivo tracking of the liposomes administered to adult zebrafish that enables evaluation of thei...

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Authors: Ruyra Ripoll, Àngels, Cano-Sarabia, Mary|||0000-0003-4254-8157, Garcia Valtanen, Pablo, Yero, Daniel|||0000-0002-6234-4082, Gibert, Isidre|||0000-0003-1442-2258, Mackenzie, Simon|||0000-0003-1845-6826, Estepa, Amparo, Maspoch Comamala, Daniel|||0000-0003-1325-9161, Roher Armentia, Nerea|||0000-0002-6659-4038
Format: article
Publication Date:2014
Country:España
Institution:Universitat Autònoma de Barcelona
Repository:Dipòsit Digital de Documents de la UAB
Language:English
OAI Identifier:oai:ddd.uab.cat:154673
Online Access:https://ddd.uab.cat/record/154673
https://dx.doi.org/urn:doi:10.1016/j.vaccine.2014.05.010
Access Level:Open access
Keyword:Immunostimulant
Infection model
Innate immunity
Liposome
Macrophageh
Nanoencapsulation
Zebrafish
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spelling Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS/dsRNA-loaded nanoliposomesRuyra Ripoll, ÀngelsCano-Sarabia, Mary|||0000-0003-4254-8157Garcia Valtanen, PabloYero, Daniel|||0000-0002-6234-4082Gibert, Isidre|||0000-0003-1442-2258Mackenzie, Simon|||0000-0003-1845-6826Estepa, AmparoMaspoch Comamala, Daniel|||0000-0003-1325-9161Roher Armentia, Nerea|||0000-0002-6659-4038ImmunostimulantInfection modelInnate immunityLiposomeMacrophagehNanoencapsulationZebrafishHerein we report the use of immunostimulant-loaded nanoliposomes (called NLcliposomes) as a strategy to protect fish against bacterial and/or viral infections. This work entailed developing a method for in vivo tracking of the liposomes administered to adult zebrafish that enables evaluation of their in vivo dynamics and characterisation of their tissue distribution. The NLc liposomes, which co-encapsulate poly(I:C) and LPS, accumulate in immune tissues and in immunologically relevant cells such as macrophages, as has been assessed in trout primary cell cultures. They protect zebrafish against otherwise lethal bacterial (Pseudomonas aeruginosa PAO1) and viral (Spring Viraemia of Carp Virus) infections regardless of whether they are administered by injection or by immersion, as demonstrated in a series of in vivo infection experiments with adult zebrafish. Importantly, protection was not achieved in fish that had been treated with empty liposomes or with a mixture of the free immunostimulants. Our findings indicate that stimulation of the innate immune system with co-encapsulated immunostimulants in nano-liposomes is a promising strategy to simultaneously improve the levels of protection against bacterial and viral infections in fish. 22014-01-0120142014-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/154673https://dx.doi.org/urn:doi:10.1016/j.vaccine.2014.05.010reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 AGL2012-33877open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.https://creativecommons.org/licenses/by-nc-nd/3.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1546732026-06-06T12:50:31Z
dc.title.none.fl_str_mv Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS/dsRNA-loaded nanoliposomes
title Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS/dsRNA-loaded nanoliposomes
spellingShingle Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS/dsRNA-loaded nanoliposomes
Ruyra Ripoll, Àngels
Immunostimulant
Infection model
Innate immunity
Liposome
Macrophageh
Nanoencapsulation
Zebrafish
title_short Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS/dsRNA-loaded nanoliposomes
title_full Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS/dsRNA-loaded nanoliposomes
title_fullStr Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS/dsRNA-loaded nanoliposomes
title_full_unstemmed Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS/dsRNA-loaded nanoliposomes
title_sort Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS/dsRNA-loaded nanoliposomes
dc.creator.none.fl_str_mv Ruyra Ripoll, Àngels
Cano-Sarabia, Mary|||0000-0003-4254-8157
Garcia Valtanen, Pablo
Yero, Daniel|||0000-0002-6234-4082
Gibert, Isidre|||0000-0003-1442-2258
Mackenzie, Simon|||0000-0003-1845-6826
Estepa, Amparo
Maspoch Comamala, Daniel|||0000-0003-1325-9161
Roher Armentia, Nerea|||0000-0002-6659-4038
author Ruyra Ripoll, Àngels
author_facet Ruyra Ripoll, Àngels
Cano-Sarabia, Mary|||0000-0003-4254-8157
Garcia Valtanen, Pablo
Yero, Daniel|||0000-0002-6234-4082
Gibert, Isidre|||0000-0003-1442-2258
Mackenzie, Simon|||0000-0003-1845-6826
Estepa, Amparo
Maspoch Comamala, Daniel|||0000-0003-1325-9161
Roher Armentia, Nerea|||0000-0002-6659-4038
author_role author
author2 Cano-Sarabia, Mary|||0000-0003-4254-8157
Garcia Valtanen, Pablo
Yero, Daniel|||0000-0002-6234-4082
Gibert, Isidre|||0000-0003-1442-2258
Mackenzie, Simon|||0000-0003-1845-6826
Estepa, Amparo
Maspoch Comamala, Daniel|||0000-0003-1325-9161
Roher Armentia, Nerea|||0000-0002-6659-4038
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Immunostimulant
Infection model
Innate immunity
Liposome
Macrophageh
Nanoencapsulation
Zebrafish
topic Immunostimulant
Infection model
Innate immunity
Liposome
Macrophageh
Nanoencapsulation
Zebrafish
description Herein we report the use of immunostimulant-loaded nanoliposomes (called NLcliposomes) as a strategy to protect fish against bacterial and/or viral infections. This work entailed developing a method for in vivo tracking of the liposomes administered to adult zebrafish that enables evaluation of their in vivo dynamics and characterisation of their tissue distribution. The NLc liposomes, which co-encapsulate poly(I:C) and LPS, accumulate in immune tissues and in immunologically relevant cells such as macrophages, as has been assessed in trout primary cell cultures. They protect zebrafish against otherwise lethal bacterial (Pseudomonas aeruginosa PAO1) and viral (Spring Viraemia of Carp Virus) infections regardless of whether they are administered by injection or by immersion, as demonstrated in a series of in vivo infection experiments with adult zebrafish. Importantly, protection was not achieved in fish that had been treated with empty liposomes or with a mixture of the free immunostimulants. Our findings indicate that stimulation of the innate immune system with co-encapsulated immunostimulants in nano-liposomes is a promising strategy to simultaneously improve the levels of protection against bacterial and viral infections in fish.
publishDate 2014
dc.date.none.fl_str_mv 2
2014-01-01
2014
2014-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/154673
https://dx.doi.org/urn:doi:10.1016/j.vaccine.2014.05.010
url https://ddd.uab.cat/record/154673
https://dx.doi.org/urn:doi:10.1016/j.vaccine.2014.05.010
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 AGL2012-33877
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/3.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/3.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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