Diversity of species and antibiotic resistance among fecal enterococci from wild birds in Tunisia. Detection of vanA-containing Enterococcus faecium isolates
Avifauna could be vectors of resistant bacteria including enterococci, but scarce information does exist about prevalence of antibiotic-resistant bacteria on bird populations, especially in the African Continent. Fecal samples of 111 birds (66 residents, 45 migratory) were taken in Tunisia in 2012....
| Autores: | , , , , , , , , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2015 |
| País: | España |
| Recursos: | Universidad de La Rioja (UR) |
| Repositorio: | RIUR. Repositorio Institucional de la Universidad de La Rioja |
| OAI Identifier: | oai:portal.dialnet.es:doc/5bbc699bb750603269e81e3b |
| Acesso em linha: | https://investigacion.unirioja.es/documentos/5bbc699bb750603269e81e3b |
| Access Level: | acceso abierto |
| Palavra-chave: | Birds E. faecium Enterococci Tunisia VanA Wild |
| Resumo: | Avifauna could be vectors of resistant bacteria including enterococci, but scarce information does exist about prevalence of antibiotic-resistant bacteria on bird populations, especially in the African Continent. Fecal samples of 111 birds (66 residents, 45 migratory) were taken in Tunisia in 2012. They were inoculated in Slanetz-Bartley agar supplemented or not with 8 μg/mL vancomycin (SB-Van and SB plates, respectively) for enterococci and vancomycin-resistant-enterococci (VRE) recovery. Enterococci were recovered in SB-plates in 73 of 111 samples (65.8 %) and one enterococci/positive sample was further studied: 60 Enterococcus faecium, 6 Enterococcus faecalis, 3 Enterococcus casseliflavus, 3 Enterococcus gallinarum, and 1 Enterococcus durans. Percentages of antimicrobial resistance identified were as follows: erythromycin (53.4 %), ciprofloxacin (41.1 %), tetracycline (19.2 %), pristanamycin (17.8 %), kanamycin, gentamicin or streptomycin (6.8–16.4 %), and ampicillin (0 %). Most of erythromycin-resistant and tetracycline-resistant enterococci carried the erm(B) and tet(M)+/−tet(L) genes, respectively. Most of high-level gentamicin or kanamycin resistant isolates carried the aac(6’)-Ie-aph(2”)-Ia or aph(3’)-IIIa genes, respectively, and 40 % of high-level streptomycin-resistant isolates the ant(6)-Ia gene. The vat(D) gene was detected in 46 % of pristinamycin-resistant enterococci and the cat(A) gene in 40 % of chloramphenicol-resistant enterococci. The gel(E), ace, or hyl genes were detected in 8–12 % of enterococci. VRE was detected in four of the birds, when SB-Van plates were used. These isolates were identified as E. faecium and carried the vanA gene, as well as the erm(B), tetM, aph (3’)-IIIa, and vatD genes, but not the hyl and esp virulence genes. Wild birds are occasional carriers of vanA-containing enterococci that could have implications in public health. |
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