Fe₃ O₄-Au Core-Shell Nanoparticles as a Multimodal Platform for in Vivo Imaging and Focused Photothermal Therapy

In this study, we report the synthesis of gold-coated iron oxide nanoparticles capped with polyvinylpyrrolidone (Fe@Au NPs). The as-synthesized nanoparticles (NPs) exhibited good stability in aqueous media and excellent features as contrast agents (CA) for both magnetic resonance imaging (MRI) and X...

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Detalles Bibliográficos
Autores: Caro Salazar, Carlos, Gámez Márquez, Francisco, Quaresma, Pedro, Páez-Muñoz, José María, Domínguez Moreno, Alejandro, Pearson, John R., Pernia Leal, Manuel, Beltrán, Ana M., Fernandez-Afonso, Yilian, Fuente, Jesús M. de la, Franco, Ricardo, Pereira, Eulália, García-Martín, María Luisa
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/106467
Acceso en línea:https://hdl.handle.net/11441/106467
https://doi.org/10.3390/pharmaceutics13030416
Access Level:acceso abierto
Palabra clave:Imaging-guided therapy
Multimodal imaging
Contrast agent
MRI
CT
Descripción
Sumario:In this study, we report the synthesis of gold-coated iron oxide nanoparticles capped with polyvinylpyrrolidone (Fe@Au NPs). The as-synthesized nanoparticles (NPs) exhibited good stability in aqueous media and excellent features as contrast agents (CA) for both magnetic resonance imaging (MRI) and X-ray computed tomography (CT). Additionally, due to the presence of the local surface plasmon resonances of gold, the NPs showed exploitable “light-to-heat” conversion ability in the near-infrared (NIR) region, a key attribute for effective photothermal therapies (PTT). In vitro experiments revealed biocompatibility as well as excellent efficiency in killing glioblastoma cells via PTT. The in vivo nontoxicity of the NPs was demonstrated using zebrafish embryos as an intermediate step between cells and rodent models. To warrant that an effective therapeutic dose was achieved inside the tumor, both intratumoral and intravenous routes were screened in rodent models by MRI and CT. The pharmacokinetics and biodistribution confirmed the multimodal imaging CA capabilities of the Fe@AuNPs and revealed constraints of the intravenous route for tumor targeting, dictating intratumoral administration for therapeutic applications. Finally, Fe@Au NPs were successfully used for an in vivo proof of concept of imaging-guided focused PTT against glioblastoma multiforme in a mouse model.

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