Evaluation of an O 2-Substituted (1-3)-β-D-Glucan, Produced by Pediococcus parvulus 2.6, in ex vivo Models of Crohn's Disease

1,3-β-glucans are extracellular polysaccharides synthesized by microorganisms and plants, with therapeutic potential. Among them, the O 2-substituted-(1-3)-β-D-glucan, synthesized by some lactic acid bacteria (LAB), has a prebiotic effect on probiotic strains, an immunomodulatory effect on monocyte-...

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Autores: Notararigo, Sara|||0000-0001-9841-5500, Varela, Encarnación, Otal, Anna, Cristobo, Iván, Antolín, María|||0000-0001-5623-6862, Guarner, Francisco|||0000-0002-4051-0836, Prieto, Alicia, López, Paloma|||0000-0001-8755-8952
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:255305
Acceso en línea:https://ddd.uab.cat/record/255305
https://dx.doi.org/urn:doi:10.3389/fmicb.2021.621280
Access Level:acceso abierto
Palabra clave:Bacterial exopolysaccharides
O2-substituted-(1-3)-β-D-glucan
Lactic acid bacteria
Immunomodulation
Crohn's disease anti-inflammatory effect
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spelling Evaluation of an O 2-Substituted (1-3)-β-D-Glucan, Produced by Pediococcus parvulus 2.6, in ex vivo Models of Crohn's DiseaseNotararigo, Sara|||0000-0001-9841-5500Varela, EncarnaciónOtal, AnnaCristobo, IvánAntolín, María|||0000-0001-5623-6862Guarner, Francisco|||0000-0002-4051-0836Prieto, AliciaLópez, Paloma|||0000-0001-8755-8952Bacterial exopolysaccharidesO2-substituted-(1-3)-β-D-glucanLactic acid bacteriaImmunomodulationCrohn's disease anti-inflammatory effect1,3-β-glucans are extracellular polysaccharides synthesized by microorganisms and plants, with therapeutic potential. Among them, the O 2-substituted-(1-3)-β-D-glucan, synthesized by some lactic acid bacteria (LAB), has a prebiotic effect on probiotic strains, an immunomodulatory effect on monocyte-derived macrophages, and potentiates the ability of the producer strain to adhere to Caco-2 cells differentiated to enterocytes. In this work, the O 2-substituted-(1-3)-β-D-glucan polymers produced by GTF glycoyltransferase in the natural host Pediococcus parvulus 2.6 and in the recombinant strain Lactococcus lactis NZ9000[pNGTF] were tested. Their immunomodulatory activity was investigated in an ex vivo model using human biopsies from patients affected by Crohn's disease (CD). Both polymers had an anti-inflammatory effect including, a reduction of Interleukine 8 both at the level of its gene expression and its secreted levels. The overall data indicate that the O 2-substituted-(1-3)-β-D-glucan have a potential role in ameliorating inflammation via the gut immune system cell modulation.Universitat Autònoma de Barcelona 22021-01-0120212021-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/255305https://dx.doi.org/urn:doi:10.3389/fmicb.2021.621280reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 RTI2018-097114-B-I00Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI12/00263open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2553052026-06-06T12:50:31Z
dc.title.none.fl_str_mv Evaluation of an O 2-Substituted (1-3)-β-D-Glucan, Produced by Pediococcus parvulus 2.6, in ex vivo Models of Crohn's Disease
title Evaluation of an O 2-Substituted (1-3)-β-D-Glucan, Produced by Pediococcus parvulus 2.6, in ex vivo Models of Crohn's Disease
spellingShingle Evaluation of an O 2-Substituted (1-3)-β-D-Glucan, Produced by Pediococcus parvulus 2.6, in ex vivo Models of Crohn's Disease
Notararigo, Sara|||0000-0001-9841-5500
Bacterial exopolysaccharides
O2-substituted-(1-3)-β-D-glucan
Lactic acid bacteria
Immunomodulation
Crohn's disease anti-inflammatory effect
title_short Evaluation of an O 2-Substituted (1-3)-β-D-Glucan, Produced by Pediococcus parvulus 2.6, in ex vivo Models of Crohn's Disease
title_full Evaluation of an O 2-Substituted (1-3)-β-D-Glucan, Produced by Pediococcus parvulus 2.6, in ex vivo Models of Crohn's Disease
title_fullStr Evaluation of an O 2-Substituted (1-3)-β-D-Glucan, Produced by Pediococcus parvulus 2.6, in ex vivo Models of Crohn's Disease
title_full_unstemmed Evaluation of an O 2-Substituted (1-3)-β-D-Glucan, Produced by Pediococcus parvulus 2.6, in ex vivo Models of Crohn's Disease
title_sort Evaluation of an O 2-Substituted (1-3)-β-D-Glucan, Produced by Pediococcus parvulus 2.6, in ex vivo Models of Crohn's Disease
dc.creator.none.fl_str_mv Notararigo, Sara|||0000-0001-9841-5500
Varela, Encarnación
Otal, Anna
Cristobo, Iván
Antolín, María|||0000-0001-5623-6862
Guarner, Francisco|||0000-0002-4051-0836
Prieto, Alicia
López, Paloma|||0000-0001-8755-8952
author Notararigo, Sara|||0000-0001-9841-5500
author_facet Notararigo, Sara|||0000-0001-9841-5500
Varela, Encarnación
Otal, Anna
Cristobo, Iván
Antolín, María|||0000-0001-5623-6862
Guarner, Francisco|||0000-0002-4051-0836
Prieto, Alicia
López, Paloma|||0000-0001-8755-8952
author_role author
author2 Varela, Encarnación
Otal, Anna
Cristobo, Iván
Antolín, María|||0000-0001-5623-6862
Guarner, Francisco|||0000-0002-4051-0836
Prieto, Alicia
López, Paloma|||0000-0001-8755-8952
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universitat Autònoma de Barcelona
dc.subject.none.fl_str_mv Bacterial exopolysaccharides
O2-substituted-(1-3)-β-D-glucan
Lactic acid bacteria
Immunomodulation
Crohn's disease anti-inflammatory effect
topic Bacterial exopolysaccharides
O2-substituted-(1-3)-β-D-glucan
Lactic acid bacteria
Immunomodulation
Crohn's disease anti-inflammatory effect
description 1,3-β-glucans are extracellular polysaccharides synthesized by microorganisms and plants, with therapeutic potential. Among them, the O 2-substituted-(1-3)-β-D-glucan, synthesized by some lactic acid bacteria (LAB), has a prebiotic effect on probiotic strains, an immunomodulatory effect on monocyte-derived macrophages, and potentiates the ability of the producer strain to adhere to Caco-2 cells differentiated to enterocytes. In this work, the O 2-substituted-(1-3)-β-D-glucan polymers produced by GTF glycoyltransferase in the natural host Pediococcus parvulus 2.6 and in the recombinant strain Lactococcus lactis NZ9000[pNGTF] were tested. Their immunomodulatory activity was investigated in an ex vivo model using human biopsies from patients affected by Crohn's disease (CD). Both polymers had an anti-inflammatory effect including, a reduction of Interleukine 8 both at the level of its gene expression and its secreted levels. The overall data indicate that the O 2-substituted-(1-3)-β-D-glucan have a potential role in ameliorating inflammation via the gut immune system cell modulation.
publishDate 2021
dc.date.none.fl_str_mv 2
2021-01-01
2021
2021-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/255305
https://dx.doi.org/urn:doi:10.3389/fmicb.2021.621280
url https://ddd.uab.cat/record/255305
https://dx.doi.org/urn:doi:10.3389/fmicb.2021.621280
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 RTI2018-097114-B-I00
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI12/00263
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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