Multimodal fluorescence microscope with fast adaptive polarimetry

Polarized light microscopy is a widely used technique to observe specimens that are optically anisotropic, or birefringent. It has a broad applicability in the study of minerals, soft materials such as polymers, complex fluids or liquid crystals, and organic tissues in biology and medicine. Most of...

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Detalles Bibliográficos
Autores: Martínez-Prat, Berta, Arteaga Barriel, Oriol, Sagués, Francesc, Ignés-Mullol, Jordi
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/219255
Acceso en línea:https://hdl.handle.net/2445/219255
Access Level:acceso abierto
Palabra clave:Fluorescència
Òptica
Microscòpia
Fluorescence
Optics
Microscopy
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spelling Multimodal fluorescence microscope with fast adaptive polarimetryMartínez-Prat, BertaArteaga Barriel, OriolSagués, FrancescIgnés-Mullol, JordiFluorescènciaÒpticaMicroscòpiaFluorescenceOpticsMicroscopyPolarized light microscopy is a widely used technique to observe specimens that are optically anisotropic, or birefringent. It has a broad applicability in the study of minerals, soft materials such as polymers, complex fluids or liquid crystals, and organic tissues in biology and medicine. Most of these observations are qualitative in nature, as it is not obvious to quantify the spatial distribution of optical anisotropy of specimens. Moreover, existing commercial implementations for quantitative polarimetry are costly and slow in nature, precluding real time observation of dynamical processes. Here, we present a custom-made implementation of an optical microscope for quantitative polarimetry at the cost of a standard scientific polarizing microscope. The instrument allows to extract the local optical axis and birefringence of transparent materials with a frequency of several Hz. The instrument is built using off-the-shelf optomechanical components, which optimizes cost, availability, and modularity. An example of the latter is the fact that we combine the polarimetry measurements with simultaneous fluorescence microscopy, which results in a powerful multimodal instrument with broad potential applications.Elsevier Ltd.2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/219255Articles publicats en revistes (Física Aplicada)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1016/j.ohx.2023.e00480HardwareX, 2023, vol. 16, p. 1-21https://doi.org/10.1016/j.ohx.2023.e00480cc-by (c) Martínez-Prat, B. et al., 2023http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2192552026-05-27T06:46:51Z
dc.title.none.fl_str_mv Multimodal fluorescence microscope with fast adaptive polarimetry
title Multimodal fluorescence microscope with fast adaptive polarimetry
spellingShingle Multimodal fluorescence microscope with fast adaptive polarimetry
Martínez-Prat, Berta
Fluorescència
Òptica
Microscòpia
Fluorescence
Optics
Microscopy
title_short Multimodal fluorescence microscope with fast adaptive polarimetry
title_full Multimodal fluorescence microscope with fast adaptive polarimetry
title_fullStr Multimodal fluorescence microscope with fast adaptive polarimetry
title_full_unstemmed Multimodal fluorescence microscope with fast adaptive polarimetry
title_sort Multimodal fluorescence microscope with fast adaptive polarimetry
dc.creator.none.fl_str_mv Martínez-Prat, Berta
Arteaga Barriel, Oriol
Sagués, Francesc
Ignés-Mullol, Jordi
author Martínez-Prat, Berta
author_facet Martínez-Prat, Berta
Arteaga Barriel, Oriol
Sagués, Francesc
Ignés-Mullol, Jordi
author_role author
author2 Arteaga Barriel, Oriol
Sagués, Francesc
Ignés-Mullol, Jordi
author2_role author
author
author
dc.subject.none.fl_str_mv Fluorescència
Òptica
Microscòpia
Fluorescence
Optics
Microscopy
topic Fluorescència
Òptica
Microscòpia
Fluorescence
Optics
Microscopy
description Polarized light microscopy is a widely used technique to observe specimens that are optically anisotropic, or birefringent. It has a broad applicability in the study of minerals, soft materials such as polymers, complex fluids or liquid crystals, and organic tissues in biology and medicine. Most of these observations are qualitative in nature, as it is not obvious to quantify the spatial distribution of optical anisotropy of specimens. Moreover, existing commercial implementations for quantitative polarimetry are costly and slow in nature, precluding real time observation of dynamical processes. Here, we present a custom-made implementation of an optical microscope for quantitative polarimetry at the cost of a standard scientific polarizing microscope. The instrument allows to extract the local optical axis and birefringence of transparent materials with a frequency of several Hz. The instrument is built using off-the-shelf optomechanical components, which optimizes cost, availability, and modularity. An example of the latter is the fact that we combine the polarimetry measurements with simultaneous fluorescence microscopy, which results in a powerful multimodal instrument with broad potential applications.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/219255
url https://hdl.handle.net/2445/219255
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1016/j.ohx.2023.e00480
HardwareX, 2023, vol. 16, p. 1-21
https://doi.org/10.1016/j.ohx.2023.e00480
dc.rights.none.fl_str_mv cc-by (c) Martínez-Prat, B. et al., 2023
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Martínez-Prat, B. et al., 2023
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier Ltd.
publisher.none.fl_str_mv Elsevier Ltd.
dc.source.none.fl_str_mv Articles publicats en revistes (Física Aplicada)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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