The MAL protein is crucial for proper membrane condensation at the ciliary base, which is required for primary cilium elongation

The base of the primary cilium contains a zone of condensedmembranes whose importance is not known. Here, we have studiedthe involvement of MAL, a tetraspanning protein that exclusivelypartitions into condensed membrane fractions, in the condensation ofmembranes at the ciliary base and investigated...

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Autores: Reales, Elena, Bernabé Rubio, Miguel, Casares Arias, Javier, Rentero Alfonso, Carles, Fernández Barrera, Jaime, Rangel, Laura, Correas, Isabel, Enrich Bastús, Carles, Andrés, Germán, Alonso, Miguel Ángel
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/166903
Acesso em linha:https://hdl.handle.net/2445/166903
Access Level:acceso abierto
Palavra-chave:Membranes cel·lulars
Proteïnes de membrana
Motilitat cel·lular
Cell membranes
Membrane proteins
Cell motility
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spelling The MAL protein is crucial for proper membrane condensation at the ciliary base, which is required for primary cilium elongationReales, ElenaBernabé Rubio, MiguelCasares Arias, JavierRentero Alfonso, CarlesFernández Barrera, JaimeRangel, LauraCorreas, IsabelEnrich Bastús, CarlesAndrés, GermánAlonso, Miguel ÁngelMembranes cel·lularsProteïnes de membranaMotilitat cel·lularCell membranesMembrane proteinsCell motilityThe base of the primary cilium contains a zone of condensedmembranes whose importance is not known. Here, we have studiedthe involvement of MAL, a tetraspanning protein that exclusivelypartitions into condensed membrane fractions, in the condensation ofmembranes at the ciliary base and investigated the importance ofthese membranes in primary cilium formation. We show that MALaccumulates at the ciliary base of epithelial MDCK cells. Knockdownof MAL expression resulted in a drastic reduction in the condensationof membranes at the ciliary base, the percentage of ciliated cells andthe length of the cilia, but did not affect the docking of the centrosometo the plasma membrane or produce missorting of proteins to thepericentriolar zone or to the membrane of the remaining cilia. Rab8(for which there are two isoforms, Rab8A and Rab8b), IFT88 andIFT20, which are important components of the machinery of ciliarygrowth, were recruited normally to the ciliary base of MAL-knockdowncells but were unable to elongate the primary cilium correctly. MAL,therefore, is crucial for the proper condensation of membranes at theciliary base, which is required for efficient primary cilium extension.The Company of Biologists2020202020152020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion10 p.application/pdfhttps://hdl.handle.net/2445/166903Articles publicats en revistes (Biomedicina)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1242/jcs.164970Journal of Cell Science, 2015, vol. 128, num. 12, p. 2261-2270https://doi.org/10.1242/jcs.164970(c) Reales, Elena et al., 2015info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1669032026-05-29T05:05:01Z
dc.title.none.fl_str_mv The MAL protein is crucial for proper membrane condensation at the ciliary base, which is required for primary cilium elongation
title The MAL protein is crucial for proper membrane condensation at the ciliary base, which is required for primary cilium elongation
spellingShingle The MAL protein is crucial for proper membrane condensation at the ciliary base, which is required for primary cilium elongation
Reales, Elena
Membranes cel·lulars
Proteïnes de membrana
Motilitat cel·lular
Cell membranes
Membrane proteins
Cell motility
title_short The MAL protein is crucial for proper membrane condensation at the ciliary base, which is required for primary cilium elongation
title_full The MAL protein is crucial for proper membrane condensation at the ciliary base, which is required for primary cilium elongation
title_fullStr The MAL protein is crucial for proper membrane condensation at the ciliary base, which is required for primary cilium elongation
title_full_unstemmed The MAL protein is crucial for proper membrane condensation at the ciliary base, which is required for primary cilium elongation
title_sort The MAL protein is crucial for proper membrane condensation at the ciliary base, which is required for primary cilium elongation
dc.creator.none.fl_str_mv Reales, Elena
Bernabé Rubio, Miguel
Casares Arias, Javier
Rentero Alfonso, Carles
Fernández Barrera, Jaime
Rangel, Laura
Correas, Isabel
Enrich Bastús, Carles
Andrés, Germán
Alonso, Miguel Ángel
author Reales, Elena
author_facet Reales, Elena
Bernabé Rubio, Miguel
Casares Arias, Javier
Rentero Alfonso, Carles
Fernández Barrera, Jaime
Rangel, Laura
Correas, Isabel
Enrich Bastús, Carles
Andrés, Germán
Alonso, Miguel Ángel
author_role author
author2 Bernabé Rubio, Miguel
Casares Arias, Javier
Rentero Alfonso, Carles
Fernández Barrera, Jaime
Rangel, Laura
Correas, Isabel
Enrich Bastús, Carles
Andrés, Germán
Alonso, Miguel Ángel
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Membranes cel·lulars
Proteïnes de membrana
Motilitat cel·lular
Cell membranes
Membrane proteins
Cell motility
topic Membranes cel·lulars
Proteïnes de membrana
Motilitat cel·lular
Cell membranes
Membrane proteins
Cell motility
description The base of the primary cilium contains a zone of condensedmembranes whose importance is not known. Here, we have studiedthe involvement of MAL, a tetraspanning protein that exclusivelypartitions into condensed membrane fractions, in the condensation ofmembranes at the ciliary base and investigated the importance ofthese membranes in primary cilium formation. We show that MALaccumulates at the ciliary base of epithelial MDCK cells. Knockdownof MAL expression resulted in a drastic reduction in the condensationof membranes at the ciliary base, the percentage of ciliated cells andthe length of the cilia, but did not affect the docking of the centrosometo the plasma membrane or produce missorting of proteins to thepericentriolar zone or to the membrane of the remaining cilia. Rab8(for which there are two isoforms, Rab8A and Rab8b), IFT88 andIFT20, which are important components of the machinery of ciliarygrowth, were recruited normally to the ciliary base of MAL-knockdowncells but were unable to elongate the primary cilium correctly. MAL,therefore, is crucial for the proper condensation of membranes at theciliary base, which is required for efficient primary cilium extension.
publishDate 2015
dc.date.none.fl_str_mv 2015
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/166903
url https://hdl.handle.net/2445/166903
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1242/jcs.164970
Journal of Cell Science, 2015, vol. 128, num. 12, p. 2261-2270
https://doi.org/10.1242/jcs.164970
dc.rights.none.fl_str_mv (c) Reales, Elena et al., 2015
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Reales, Elena et al., 2015
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10 p.
application/pdf
dc.publisher.none.fl_str_mv The Company of Biologists
publisher.none.fl_str_mv The Company of Biologists
dc.source.none.fl_str_mv Articles publicats en revistes (Biomedicina)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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