Effects of nanocrystalline hydroxyapatites on macrophage polarization

Silicon substituted and nanocrystalline hydroxyapatites have attracted the attention of many researchers due to their up-regulation in osteoblast cell metabolism and enhanced bioreactivity, respectively. On the other hand, the biomaterial success or failure depends ultimately on the immune response...

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Autores: Linares, J., Fernández, A. B., Feito Castellano, María José, Matesanz Sancho, María Concepción, Sánchez-Salcedo, Sandra, Arcos Navarrete, Daniel, Vallet Regí, María Dulce Nombre, Rojo, J.M., Portolés Pérez, María Teresa
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/23087
Acceso en línea:https://hdl.handle.net/20.500.14352/23087
Access Level:acceso abierto
Palabra clave:546
615.46
Silicon-substituted hydroxyapatite
Signaling pathways
Activation
Bioceramics
Osteoblasts
Lymphocytes
Plasticity
Medicine
Behavior
Materiales
Química inorgánica (Química)
3312 Tecnología de Materiales
2303 Química Inorgánica
id ES_938cfb8f9316268a6d2d127d07c95b4e
oai_identifier_str oai:docta.ucm.es:20.500.14352/23087
network_acronym_str ES
network_name_str España
repository_id_str
spelling Effects of nanocrystalline hydroxyapatites on macrophage polarizationLinares, J.Fernández, A. B.Feito Castellano, María JoséMatesanz Sancho, María ConcepciónSánchez-Salcedo, SandraArcos Navarrete, DanielVallet Regí, María Dulce NombreRojo, J.M.Portolés Pérez, María Teresa546615.46Silicon-substituted hydroxyapatiteSignaling pathwaysActivationBioceramicsOsteoblastsLymphocytesPlasticityMedicineBehaviorMaterialesQuímica inorgánica (Química)3312 Tecnología de Materiales2303 Química InorgánicaSilicon substituted and nanocrystalline hydroxyapatites have attracted the attention of many researchers due to their up-regulation in osteoblast cell metabolism and enhanced bioreactivity, respectively. On the other hand, the biomaterial success or failure depends ultimately on the immune response triggered after its implantation. Macrophages are the main components of the innate immune system with an important role in healing and tissue remodelling due to their remarkable functional plasticity, existing in a whole spectrum of functional populations with varying phenotypic features. The effects of nanocrystalline hydroxyapatite (nano-HA) and nanocrystalline silicon substituted hydroxyapatite (nano-SiHA) on the macrophage populations defined as pro-inflammatory (M1) and reparative (M2) phenotypes have been evaluated in the present study using RAW 264.7 cells and mouse peritoneal macrophages as in vitro models. M1 and M2 macrophage phenotypes were characterized by flow cytometry and confocal microscopy by the expression of CD80 and CD163, known as M1 and M2 markers, respectively. The polarization of primary macrophages towards the M1 or M2 phenotype was induced with the pro-inflammatory stimulus LPS or the anti-inflammatory stimulus IL-10, respectively, evaluating the biomaterial effects under these conditions. Our results show that both nano-HA and nano-SiHA favour the macrophage polarization towards an M2 reparative phenotype, decreasing M1 population and ensuring an appropriate response in the implantation site of these biomaterials designed for bone repair and bone tissue engineering.Royal Society of ChemistryUniversidad Complutense de Madrid20162016-01-0120162016-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/23087reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/230872026-06-02T12:44:21Z
dc.title.none.fl_str_mv Effects of nanocrystalline hydroxyapatites on macrophage polarization
title Effects of nanocrystalline hydroxyapatites on macrophage polarization
spellingShingle Effects of nanocrystalline hydroxyapatites on macrophage polarization
Linares, J.
546
615.46
Silicon-substituted hydroxyapatite
Signaling pathways
Activation
Bioceramics
Osteoblasts
Lymphocytes
Plasticity
Medicine
Behavior
Materiales
Química inorgánica (Química)
3312 Tecnología de Materiales
2303 Química Inorgánica
title_short Effects of nanocrystalline hydroxyapatites on macrophage polarization
title_full Effects of nanocrystalline hydroxyapatites on macrophage polarization
title_fullStr Effects of nanocrystalline hydroxyapatites on macrophage polarization
title_full_unstemmed Effects of nanocrystalline hydroxyapatites on macrophage polarization
title_sort Effects of nanocrystalline hydroxyapatites on macrophage polarization
dc.creator.none.fl_str_mv Linares, J.
Fernández, A. B.
Feito Castellano, María José
Matesanz Sancho, María Concepción
Sánchez-Salcedo, Sandra
Arcos Navarrete, Daniel
Vallet Regí, María Dulce Nombre
Rojo, J.M.
Portolés Pérez, María Teresa
author Linares, J.
author_facet Linares, J.
Fernández, A. B.
Feito Castellano, María José
Matesanz Sancho, María Concepción
Sánchez-Salcedo, Sandra
Arcos Navarrete, Daniel
Vallet Regí, María Dulce Nombre
Rojo, J.M.
Portolés Pérez, María Teresa
author_role author
author2 Fernández, A. B.
Feito Castellano, María José
Matesanz Sancho, María Concepción
Sánchez-Salcedo, Sandra
Arcos Navarrete, Daniel
Vallet Regí, María Dulce Nombre
Rojo, J.M.
Portolés Pérez, María Teresa
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 546
615.46
Silicon-substituted hydroxyapatite
Signaling pathways
Activation
Bioceramics
Osteoblasts
Lymphocytes
Plasticity
Medicine
Behavior
Materiales
Química inorgánica (Química)
3312 Tecnología de Materiales
2303 Química Inorgánica
topic 546
615.46
Silicon-substituted hydroxyapatite
Signaling pathways
Activation
Bioceramics
Osteoblasts
Lymphocytes
Plasticity
Medicine
Behavior
Materiales
Química inorgánica (Química)
3312 Tecnología de Materiales
2303 Química Inorgánica
description Silicon substituted and nanocrystalline hydroxyapatites have attracted the attention of many researchers due to their up-regulation in osteoblast cell metabolism and enhanced bioreactivity, respectively. On the other hand, the biomaterial success or failure depends ultimately on the immune response triggered after its implantation. Macrophages are the main components of the innate immune system with an important role in healing and tissue remodelling due to their remarkable functional plasticity, existing in a whole spectrum of functional populations with varying phenotypic features. The effects of nanocrystalline hydroxyapatite (nano-HA) and nanocrystalline silicon substituted hydroxyapatite (nano-SiHA) on the macrophage populations defined as pro-inflammatory (M1) and reparative (M2) phenotypes have been evaluated in the present study using RAW 264.7 cells and mouse peritoneal macrophages as in vitro models. M1 and M2 macrophage phenotypes were characterized by flow cytometry and confocal microscopy by the expression of CD80 and CD163, known as M1 and M2 markers, respectively. The polarization of primary macrophages towards the M1 or M2 phenotype was induced with the pro-inflammatory stimulus LPS or the anti-inflammatory stimulus IL-10, respectively, evaluating the biomaterial effects under these conditions. Our results show that both nano-HA and nano-SiHA favour the macrophage polarization towards an M2 reparative phenotype, decreasing M1 population and ensuring an appropriate response in the implantation site of these biomaterials designed for bone repair and bone tissue engineering.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01
2016
2016-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/23087
url https://hdl.handle.net/20.500.14352/23087
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869413606447120384
score 15,300719

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