Kinetics of Indigenous Nitrate Reducing Sulfide Oxidizing Activity in Microaerophilic Wastewater Biofilms

23 páginas.-- 10 figuras.-- 2 tablas.-- 74 referencias.-- Supporting Information: Dataset. in http://dx.doi.org/10.1371/journal.pone.0149096

Detalles Bibliográficos
Autores: Villahermosa, Desiré, Corzo Rodríguez, Alfonso, García-Robledo, Emilio, González Grau, Juan Miguel, Papaspyrou, Sokratis
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/130432
Acceso en línea:http://hdl.handle.net/10261/130432
Access Level:acceso abierto
Palabra clave:Sulfates
Nitrates
Biofilms
Sulfides
Oxidation
Nitrites
Bacterial biofilms
Oxygen
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spelling Kinetics of Indigenous Nitrate Reducing Sulfide Oxidizing Activity in Microaerophilic Wastewater BiofilmsVillahermosa, DesiréCorzo Rodríguez, AlfonsoGarcía-Robledo, EmilioGonzález Grau, Juan MiguelPapaspyrou, SokratisSulfatesNitratesBiofilmsSulfidesOxidationNitritesBacterial biofilmsOxygen23 páginas.-- 10 figuras.-- 2 tablas.-- 74 referencias.-- Supporting Information: Dataset. in http://dx.doi.org/10.1371/journal.pone.0149096Nitrate decreases sulfide release in wastewater treatment plants (WWTP), but little is known on how it affects the microzonation and kinetics of related microbial processes within the biofilm. The effect of nitrate addition on these properties for sulfate reduction, sulfide oxidation, and oxygen respiration were studied with the use of microelectrodes in microaerophilic wastewater biofilms. Mass balance calaculations and community composition analysis were also performed. At basal WWTP conditions, the biofilm presented a double-layer system. The upper microaerophilic layer (~300 μm) showed low sulfide production (0.31 μmol cm-3 h-1) and oxygen consumption rates (0.01 μmol cm-3 h-1). The anoxic lower layer showed high sulfide production (2.7 μmol cm-3 h-1). Nitrate addition decreased net sulfide production rates, caused by an increase in sulfide oxidation rates (SOR) in the upper layer, rather than an inhibition of sulfate reducing bacteria (SRB). This suggests that the indigenous nitrate reducing-sulfide oxidizing bacteria (NR-SOB) were immediately activated by nitrate. The functional vertical structure of the biofilm changed to a triple-layer system, where the previously upper sulfide-producing layer in the absence of nitrate split into two new layers: 1) an upper sulfide-consuming layer, whose thickness is probably determined by the nitrate penetration depth within the biofilm, and 2) a middle layer producing sulfide at an even higher rate than in the absence of nitrate in some cases. Below these layers, the lower net sulfide-producing layer remained unaffected. Net SOR varied from 0.05 to 0.72 μmol cm-3 h-1 depending on nitrate and sulfate availability. Addition of low nitrate concentrations likely increased sulfate availability within the biofilm and resulted in an increase of both net sulfate reduction and net sulfide oxidation by overcoming sulfate diffusional limitation from the water phase and the strong coupling between SRB and NR-SOB syntrophic relationshipAC was funded by projects P06-RNM-01787, P11-RNM-7199, the PAI group RNM-214 from Consejería de Innovación, Ciencia y Empresa, Junta de Andalucía and CTM2013-43857-R from the Spanish Ministry of Economy and Competitiveness. JMG was funded by the PAI group BIO-288 from Consejería de Innovación, Ciencia y Empresa, Junta de Andalucía. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Peer reviewedPublic Library of ScienceJunta de AndalucíaMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/130432reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTM2013-43857-Rhttp://dx.doi.org/10.1371/journal.pone.0149096Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1304322026-05-22T06:33:51Z
dc.title.none.fl_str_mv Kinetics of Indigenous Nitrate Reducing Sulfide Oxidizing Activity in Microaerophilic Wastewater Biofilms
title Kinetics of Indigenous Nitrate Reducing Sulfide Oxidizing Activity in Microaerophilic Wastewater Biofilms
spellingShingle Kinetics of Indigenous Nitrate Reducing Sulfide Oxidizing Activity in Microaerophilic Wastewater Biofilms
Villahermosa, Desiré
Sulfates
Nitrates
Biofilms
Sulfides
Oxidation
Nitrites
Bacterial biofilms
Oxygen
title_short Kinetics of Indigenous Nitrate Reducing Sulfide Oxidizing Activity in Microaerophilic Wastewater Biofilms
title_full Kinetics of Indigenous Nitrate Reducing Sulfide Oxidizing Activity in Microaerophilic Wastewater Biofilms
title_fullStr Kinetics of Indigenous Nitrate Reducing Sulfide Oxidizing Activity in Microaerophilic Wastewater Biofilms
title_full_unstemmed Kinetics of Indigenous Nitrate Reducing Sulfide Oxidizing Activity in Microaerophilic Wastewater Biofilms
title_sort Kinetics of Indigenous Nitrate Reducing Sulfide Oxidizing Activity in Microaerophilic Wastewater Biofilms
dc.creator.none.fl_str_mv Villahermosa, Desiré
Corzo Rodríguez, Alfonso
García-Robledo, Emilio
González Grau, Juan Miguel
Papaspyrou, Sokratis
author Villahermosa, Desiré
author_facet Villahermosa, Desiré
Corzo Rodríguez, Alfonso
García-Robledo, Emilio
González Grau, Juan Miguel
Papaspyrou, Sokratis
author_role author
author2 Corzo Rodríguez, Alfonso
García-Robledo, Emilio
González Grau, Juan Miguel
Papaspyrou, Sokratis
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Junta de Andalucía
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Sulfates
Nitrates
Biofilms
Sulfides
Oxidation
Nitrites
Bacterial biofilms
Oxygen
topic Sulfates
Nitrates
Biofilms
Sulfides
Oxidation
Nitrites
Bacterial biofilms
Oxygen
description 23 páginas.-- 10 figuras.-- 2 tablas.-- 74 referencias.-- Supporting Information: Dataset. in http://dx.doi.org/10.1371/journal.pone.0149096
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/130432
url http://hdl.handle.net/10261/130432
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTM2013-43857-R
http://dx.doi.org/10.1371/journal.pone.0149096
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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