A higher proportion of eicosapentaenoic acid (EPA) when combined with docosahexaenoic acid (DHA) in omega-3 dietary supplements provides higher antioxidant effects in human retinal cells

Retinal pigment epithelium (RPE) is a key regulator of retinal function and is directly related to the transport, delivery, and metabolism of long-chain n-3 polyunsaturated fatty acids (n3-PUFA), in the retina. Due to their functions and location, RPE cells are constantly exposed to oxidative stress...

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Detalles Bibliográficos
Autores: Saenz-de-Viteri, M. (Manuel)|||/items/be779c9a-c9bd-4ecd-96aa-0e9970e49c33, Hernandez, M. (María)|||/items/64ef42cb-3438-4cd6-b8db-8a01244368da, Bilbao-Malavé, V. (Valentina)|||/items/024d213a-2062-473e-8ce1-949c6e2ad785, Fernandez-Robredo, P. (Patricia)|||/items/cebb4451-3415-44cf-811a-c5ae0b139604, González-Zamora, J. (Jorge)|||/items/8ac4ee56-6070-49a3-b32f-5d3e8f9eef17, Garcia-Garcia, L. (Laura)|||/items/c0a0bfa6-658c-4340-a6eb-3d19a987c4ab, Ispizua, N. (Nahia)|||/items/1bac8095-e254-4562-8ce7-880562bbc175, Recalde-Maestre, S. (Sergio)|||/items/1f021661-e027-480f-bd57-ce3bfb8e5a0e, Garcia-Layana, A. (Alfredo)|||/items/631fff44-be4e-42e5-ad3a-8f0f10305836
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/65410
Acceso en línea:https://hdl.handle.net/10171/65410
Access Level:acceso abierto
Palabra clave:Eicosapentaenoic acid (EPA)
Docosahexaenoic acid (DHA)
Oxidative stress
Diabetic retinopathy
Retinal pigment epithelium
Descripción
Sumario:Retinal pigment epithelium (RPE) is a key regulator of retinal function and is directly related to the transport, delivery, and metabolism of long-chain n-3 polyunsaturated fatty acids (n3-PUFA), in the retina. Due to their functions and location, RPE cells are constantly exposed to oxidative stress. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have shown to have antioxidant effects by different mechanisms. For this reason, we designed an in vitro study to compare 10 formulations of DHA and EPA supplements from different origins and combined in different proportions, evaluating their effect on cell viability, cell proliferation, reactive oxygen species production, and cell migration using ARPE-19 cells. Furthermore, we assessed their ability to rescue RPE cells from the oxidative conditions seen in diabetic retinopathy. Our results showed that the different formulations of n3-PUFAs have a beneficial effect on cell viability and proliferation and are able to restore oxidative induced RPE damage. We observed that the n3-PUFA provided different results alone or combined in the same supplement. When combined, the best results were obtained in formulations that included a higher proportion of EPA than DHA. Moreover, n3-PUFA in the form of ethyl-esters had a worse performance when compared with triglycerides or phospholipid based formulations.

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