Dual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function Analysis

Improved methods for manipulating and analyzing gene function have provided a better understanding of how genes work during organ development and disease. Inducible functional genetic mosaics can be extraordinarily useful in the study of biological systems; however, this experimental approach is sti...

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Authors: Pontes-Quero, Samuel, Heredia, Luis, Casquero-García, Verónica, Fernández-Chacón, Macarena, Luo, Wen, Hermoso, Ana, Bansal, Mayank, Garcia-Gonzalez, Irene, Sanchez-Muñoz, Maria S., Perea, Juan R., Galiana-Simal, Adrian, Rodriguez-Arabaolaza, Iker, Del Olmo-Cabrera, Sergio, Rocha, Susana F., Criado-Rodriguez, Luis M., Giovinazzo, Giovanna, Benedito, Rui
Format: article
Publication Date:2017
Country:España
Institution:Universidad Autónoma de Madrid
Repository:Biblos-e Archivo. Repositorio Institucional de la UAM
Language:English
OAI Identifier:oai:repositorio.uam.es:10486/685831
Online Access:http://hdl.handle.net/10486/685831
https://dx.doi.org/10.1016/j.cell.2017.07.031
Access Level:Open access
Keyword:Angiogenesis
Brainbow
Confetti
Epistasis
Mosaic
Notch
Rosa26
Transgenesis
VEGF
Medicina
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spelling Dual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function AnalysisPontes-Quero, SamuelHeredia, LuisCasquero-García, VerónicaFernández-Chacón, MacarenaLuo, WenHermoso, AnaBansal, MayankGarcia-Gonzalez, IreneSanchez-Muñoz, Maria S.Perea, Juan R.Galiana-Simal, AdrianRodriguez-Arabaolaza, IkerDel Olmo-Cabrera, SergioRocha, Susana F.Criado-Rodriguez, Luis M.Giovinazzo, GiovannaBenedito, RuiAngiogenesisBrainbowConfettiEpistasisMosaicNotchRosa26TransgenesisVEGFMedicinaImproved methods for manipulating and analyzing gene function have provided a better understanding of how genes work during organ development and disease. Inducible functional genetic mosaics can be extraordinarily useful in the study of biological systems; however, this experimental approach is still rarely used in vertebrates. This is mainly due to technical difficulties in the assembly of large DNA constructs carrying multiple genes and regulatory elements and their targeting to the genome. In addition, mosaic phenotypic analysis, unlike classical single gene-function analysis, requires clear labeling and detection of multiple cell clones in the same tissue. Here, we describe several methods for the rapid generation of transgenic or gene-targeted mice and embryonic stem (ES) cell lines containing all the necessary elements for inducible, fluorescent, and functional genetic mosaic (ifgMosaic) analysis. This technology enables the interrogation of multiple and combinatorial gene function with high temporal and cellular resolution.This work was supported by grants to the PI R.B. from the Spanish Ministry of Economy, Industry and Competitiveness (SAF2013-44329-P, SAF2013- 42359-ERC, and RYC-2013-13209) and European Research Council (ERC- 2014-StG - 638028). S.P.-Q., M.F.-C., and I.G.-G. were supported by PhD fellowships from Fundacio´ n La Caixa (CX-SO-2013-02, CX_E-2015-01, and CX-SO-16-1, respectively). W.L. by a FP7-PEOPLE-2012-COFUND GA600396 postdoctoral contract.Published by Elsevier Inc.Departamento de BioquímicaFacultad de Medicina20172017-08-10research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/685831https://dx.doi.org/10.1016/j.cell.2017.07.031reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/6858312026-06-23T12:46:27Z
dc.title.none.fl_str_mv Dual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function Analysis
title Dual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function Analysis
spellingShingle Dual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function Analysis
Pontes-Quero, Samuel
Angiogenesis
Brainbow
Confetti
Epistasis
Mosaic
Notch
Rosa26
Transgenesis
VEGF
Medicina
title_short Dual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function Analysis
title_full Dual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function Analysis
title_fullStr Dual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function Analysis
title_full_unstemmed Dual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function Analysis
title_sort Dual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function Analysis
dc.creator.none.fl_str_mv Pontes-Quero, Samuel
Heredia, Luis
Casquero-García, Verónica
Fernández-Chacón, Macarena
Luo, Wen
Hermoso, Ana
Bansal, Mayank
Garcia-Gonzalez, Irene
Sanchez-Muñoz, Maria S.
Perea, Juan R.
Galiana-Simal, Adrian
Rodriguez-Arabaolaza, Iker
Del Olmo-Cabrera, Sergio
Rocha, Susana F.
Criado-Rodriguez, Luis M.
Giovinazzo, Giovanna
Benedito, Rui
author Pontes-Quero, Samuel
author_facet Pontes-Quero, Samuel
Heredia, Luis
Casquero-García, Verónica
Fernández-Chacón, Macarena
Luo, Wen
Hermoso, Ana
Bansal, Mayank
Garcia-Gonzalez, Irene
Sanchez-Muñoz, Maria S.
Perea, Juan R.
Galiana-Simal, Adrian
Rodriguez-Arabaolaza, Iker
Del Olmo-Cabrera, Sergio
Rocha, Susana F.
Criado-Rodriguez, Luis M.
Giovinazzo, Giovanna
Benedito, Rui
author_role author
author2 Heredia, Luis
Casquero-García, Verónica
Fernández-Chacón, Macarena
Luo, Wen
Hermoso, Ana
Bansal, Mayank
Garcia-Gonzalez, Irene
Sanchez-Muñoz, Maria S.
Perea, Juan R.
Galiana-Simal, Adrian
Rodriguez-Arabaolaza, Iker
Del Olmo-Cabrera, Sergio
Rocha, Susana F.
Criado-Rodriguez, Luis M.
Giovinazzo, Giovanna
Benedito, Rui
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Bioquímica
Facultad de Medicina
dc.subject.none.fl_str_mv Angiogenesis
Brainbow
Confetti
Epistasis
Mosaic
Notch
Rosa26
Transgenesis
VEGF
Medicina
topic Angiogenesis
Brainbow
Confetti
Epistasis
Mosaic
Notch
Rosa26
Transgenesis
VEGF
Medicina
description Improved methods for manipulating and analyzing gene function have provided a better understanding of how genes work during organ development and disease. Inducible functional genetic mosaics can be extraordinarily useful in the study of biological systems; however, this experimental approach is still rarely used in vertebrates. This is mainly due to technical difficulties in the assembly of large DNA constructs carrying multiple genes and regulatory elements and their targeting to the genome. In addition, mosaic phenotypic analysis, unlike classical single gene-function analysis, requires clear labeling and detection of multiple cell clones in the same tissue. Here, we describe several methods for the rapid generation of transgenic or gene-targeted mice and embryonic stem (ES) cell lines containing all the necessary elements for inducible, fluorescent, and functional genetic mosaic (ifgMosaic) analysis. This technology enables the interrogation of multiple and combinatorial gene function with high temporal and cellular resolution.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-08-10
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/685831
https://dx.doi.org/10.1016/j.cell.2017.07.031
url http://hdl.handle.net/10486/685831
https://dx.doi.org/10.1016/j.cell.2017.07.031
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Published by Elsevier Inc.
publisher.none.fl_str_mv Published by Elsevier Inc.
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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