Degradation of IF1 controls energy metabolism during osteogenic differentiation of stem cells

Differentiation of human mesenchymal stem cells (hMSCs) requires the rewiring of energy metabolism. Herein, we demonstrate that the ATPase inhibitory factor 1 (IF1) is expressed in hMSCs and in prostate and colon stem cells but is not expressed in the differentiated cells. IF1 inhibits oxidative pho...

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Detalles Bibliográficos
Autores: Sánchez-Aragó, María, García-Bermúdez, Javier, Martínez-Reyes, Inmaculada, Santacatterina, Fulvio, Cuezva Marcos, José Manuel
Tipo de recurso: artículo
Fecha de publicación:2013
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/666832
Acceso en línea:http://hdl.handle.net/10486/666832
https://dx.doi.org/10.1038/embor.2013.72
Access Level:acceso abierto
Palabra clave:ATPase inhibitory factor 1
cellular differentiation
H+-ATP synthase
mitochondria
protein degradation
Biología y Biomedicina / Biología
Descripción
Sumario:Differentiation of human mesenchymal stem cells (hMSCs) requires the rewiring of energy metabolism. Herein, we demonstrate that the ATPase inhibitory factor 1 (IF1) is expressed in hMSCs and in prostate and colon stem cells but is not expressed in the differentiated cells. IF1 inhibits oxidative phosphorylation and regulates the activity of aerobic glycolysis in hMSCs. Silencing of IF1 in hMSCs mimics the metabolic changes observed in osteocytes and accelerates cellular differentiation. Activation of IF1 degradation acts as the switch that regulates energy metabolism during differentiation. We conclude that IF1 is a stemness marker important for maintaining the quiescence state