Multiply charged ions profile in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata blooms

Analogues of palytoxin (PLTX), one of the most potent marine biotoxins, are produced by some species of the marine dinoflagellates of the genus Ostreopsis. The proliferation of these species in different coastal zones represents a potential threat of seafood poisoning in humans because the produced...

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Autores: Medina-Pérez, Noemí I., Santos Vicente, Francisco Javier, Berdalet, Elisa, Moyano Morcillo, Encarnación
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/217045
Acceso en línea:https://hdl.handle.net/2445/217045
Access Level:acceso abierto
Palabra clave:Cromatografia de líquids d'alta resolució
Vasoconstrictors
High performance liquid chromatography
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spelling Multiply charged ions profile in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata bloomsMedina-Pérez, Noemí I.Santos Vicente, Francisco JavierBerdalet, ElisaMoyano Morcillo, EncarnaciónCromatografia de líquids d'alta resolucióVasoconstrictorsHigh performance liquid chromatographyVasoconstrictorsAnalogues of palytoxin (PLTX), one of the most potent marine biotoxins, are produced by some species of the marine dinoflagellates of the genus Ostreopsis. The proliferation of these species in different coastal zones represents a potential threat of seafood poisoning in humans because the produced toxins can be transferred through the marine food webs. Thus, the determination of the concentration of PLTX analogues (ovatoxins- OVTXs, ostreocins-OSTs and isobaric PLTX) in different matrices (seawater, marine fauna, etc.) is necessary to protect human health. This study is addressed to overcome some of the challenges that the chemical complexity of these molecules poses to their quantification by ultra-high-performance liquid chromatography high-resolution mass spectrometry-based techniques (UHPLC-HRMS). In particular, the mass spectrum of the palytoxin analogues shows the presence of a large number of ions (including mono- and multiply-charged ions) whose nature, relative abundances and behavior can lead to quantitation errors if the correct ions are not selected. In this work, the variability of the PLTX and OVTXs profiles under different instrument conditions, including the use of diverse electrospray generation sources and different quantitation methods, is studied. Moreover, the extraction protocol in seawater containing Ostreopsis sp. ovata cells is also evaluated. The use of a heated electrospray operating at 350 °C and a quantitative method including ions from different multiplycharged species provides a more robust and reliable method overcoming the problems due to the variability in the toxin's mass spectrum profile. A single MeOH:H2O (80:20, v/v) extraction is proposed as the best and reliable procedure. The overall method proposed was applied to quantify OVTXs (-a to -g) and iso-PLTX along the 2019 Ostreopsis cf. ovata bloom. The cells contained a total toxin concentration up to 20.39 pg cell-1.Royal Society of Chemistry2024202420232024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion10 p.application/pdfhttps://hdl.handle.net/2445/217045Articles publicats en revistes (Enginyeria Química i Química Analítica)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1039/d2ay02019jAnalytical Methods, 2023, vol. 15, p. 1355-1364https://doi.org/10.1039/d2ay02019jcc-by-nc (c) Medina-Pérez, N. I. et al., 2023http://creativecommons.org/licenses/by-nc/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2170452026-05-29T05:05:01Z
dc.title.none.fl_str_mv Multiply charged ions profile in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata blooms
title Multiply charged ions profile in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata blooms
spellingShingle Multiply charged ions profile in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata blooms
Medina-Pérez, Noemí I.
Cromatografia de líquids d'alta resolució
Vasoconstrictors
High performance liquid chromatography
Vasoconstrictors
title_short Multiply charged ions profile in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata blooms
title_full Multiply charged ions profile in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata blooms
title_fullStr Multiply charged ions profile in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata blooms
title_full_unstemmed Multiply charged ions profile in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata blooms
title_sort Multiply charged ions profile in the UHPLC-HRMS analysis of palytoxin analogues from Ostreopsis cf. ovata blooms
dc.creator.none.fl_str_mv Medina-Pérez, Noemí I.
Santos Vicente, Francisco Javier
Berdalet, Elisa
Moyano Morcillo, Encarnación
author Medina-Pérez, Noemí I.
author_facet Medina-Pérez, Noemí I.
Santos Vicente, Francisco Javier
Berdalet, Elisa
Moyano Morcillo, Encarnación
author_role author
author2 Santos Vicente, Francisco Javier
Berdalet, Elisa
Moyano Morcillo, Encarnación
author2_role author
author
author
dc.subject.none.fl_str_mv Cromatografia de líquids d'alta resolució
Vasoconstrictors
High performance liquid chromatography
Vasoconstrictors
topic Cromatografia de líquids d'alta resolució
Vasoconstrictors
High performance liquid chromatography
Vasoconstrictors
description Analogues of palytoxin (PLTX), one of the most potent marine biotoxins, are produced by some species of the marine dinoflagellates of the genus Ostreopsis. The proliferation of these species in different coastal zones represents a potential threat of seafood poisoning in humans because the produced toxins can be transferred through the marine food webs. Thus, the determination of the concentration of PLTX analogues (ovatoxins- OVTXs, ostreocins-OSTs and isobaric PLTX) in different matrices (seawater, marine fauna, etc.) is necessary to protect human health. This study is addressed to overcome some of the challenges that the chemical complexity of these molecules poses to their quantification by ultra-high-performance liquid chromatography high-resolution mass spectrometry-based techniques (UHPLC-HRMS). In particular, the mass spectrum of the palytoxin analogues shows the presence of a large number of ions (including mono- and multiply-charged ions) whose nature, relative abundances and behavior can lead to quantitation errors if the correct ions are not selected. In this work, the variability of the PLTX and OVTXs profiles under different instrument conditions, including the use of diverse electrospray generation sources and different quantitation methods, is studied. Moreover, the extraction protocol in seawater containing Ostreopsis sp. ovata cells is also evaluated. The use of a heated electrospray operating at 350 °C and a quantitative method including ions from different multiplycharged species provides a more robust and reliable method overcoming the problems due to the variability in the toxin's mass spectrum profile. A single MeOH:H2O (80:20, v/v) extraction is proposed as the best and reliable procedure. The overall method proposed was applied to quantify OVTXs (-a to -g) and iso-PLTX along the 2019 Ostreopsis cf. ovata bloom. The cells contained a total toxin concentration up to 20.39 pg cell-1.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/217045
url https://hdl.handle.net/2445/217045
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1039/d2ay02019j
Analytical Methods, 2023, vol. 15, p. 1355-1364
https://doi.org/10.1039/d2ay02019j
dc.rights.none.fl_str_mv cc-by-nc (c) Medina-Pérez, N. I. et al., 2023
http://creativecommons.org/licenses/by-nc/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc (c) Medina-Pérez, N. I. et al., 2023
http://creativecommons.org/licenses/by-nc/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10 p.
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv Articles publicats en revistes (Enginyeria Química i Química Analítica)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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