Comparative analysis of different methods for protein quantification in donated human milk

Background: Human milk is the best option for feeding newborns, especially premature infants. In the absence of breast milk, milk from a human milk bank can be a suitable alternative. However, the nutritional content of human milk may be insufficient to meet these high requirements and milk fortific...

Descripción completa

Detalles Bibliográficos
Autores: Navarro Tapia, Elisabet, Herranz Barbero, Ana, Marquina, Maribel, Borrás-Novell, Cristina, Pleguezuelos, Vanessa, Candel Vila, Rafael, García Algar, Óscar, Andreu Fernández, Vicente
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/215908
Acceso en línea:https://hdl.handle.net/2445/215908
Access Level:acceso abierto
Palabra clave:Alimentació dels nodrissons
Llet materna
Proteïnes de la llet
Infants prematurs
Nodrissons
Infants nutrition
Breast milk
Milk proteins
Premature infants
Infants
id ES_92a2d31ff149d9f7dbb680e4de3c95a7
oai_identifier_str oai:diposit.ub.edu:2445/215908
network_acronym_str ES
network_name_str España
repository_id_str
spelling Comparative analysis of different methods for protein quantification in donated human milkNavarro Tapia, ElisabetHerranz Barbero, AnaMarquina, MaribelBorrás-Novell, CristinaPleguezuelos, VanessaCandel Vila, RafaelGarcía Algar, ÓscarAndreu Fernández, VicenteAlimentació dels nodrissonsLlet maternaProteïnes de la lletInfants prematursNodrissonsInfants nutritionBreast milkMilk proteinsPremature infantsInfantsBackground: Human milk is the best option for feeding newborns, especially premature infants. In the absence of breast milk, milk from a human milk bank can be a suitable alternative. However, the nutritional content of human milk may be insufficient to meet these high requirements and milk fortification is needed. To facilitate the implementation of simpler and faster analyzers in neonatal healthcare facilities, this study focuses on the concordance analysis of two different analyzers, one based on mid-infrared and the other on ultrasound, in comparison to the Bradford method for determining protein concentration in human milk. Methods: Mature milk samples from donor mothers were collected and pasteurized at the Human Milk Bank of Barcelona and protein quantification was performed using mid-infrared (MIRIS-HMA), ultrasound (MilkoScope Julie27), and the classical Bradford reference methods. The intraclass correlation coefficient (ICC) with 95% confidence interval and Bland-Altman plots were used to assess the agreement between methods. Results: The mean protein concentration of 142 milk samples calculated using MIRIS-HMA, MilkoScope, and the Bradford assay were 1.38, 1.15, and 1.19 g/100 ml, respectively. The ICC was 0.70 for MIRIS-HMA vs. Bradford and 0.37 for MilkoScope vs. Bradford. Conclusion: MIRIS-HMA obtained a better agreement with the Bradford technique and is a promising method for developing new devices based on MIR transmission spectroscopy principles. This study confirms how MIRIS-HMA can be used to accurately calculate the protein concentration of human milk.Frontiers Media2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/215908Articles publicats en revistes (BCNatal Fetal Medicine Research Center)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3389/fped.2024.1436885Frontiers in Pediatrics, 2024, vol. 12, 1436885https://doi.org/10.3389/fped.2024.1436885cc-by (c) Elisabet Navarro-Tapia et al., 2024http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2159082026-05-27T06:46:51Z
dc.title.none.fl_str_mv Comparative analysis of different methods for protein quantification in donated human milk
title Comparative analysis of different methods for protein quantification in donated human milk
spellingShingle Comparative analysis of different methods for protein quantification in donated human milk
Navarro Tapia, Elisabet
Alimentació dels nodrissons
Llet materna
Proteïnes de la llet
Infants prematurs
Nodrissons
Infants nutrition
Breast milk
Milk proteins
Premature infants
Infants
title_short Comparative analysis of different methods for protein quantification in donated human milk
title_full Comparative analysis of different methods for protein quantification in donated human milk
title_fullStr Comparative analysis of different methods for protein quantification in donated human milk
title_full_unstemmed Comparative analysis of different methods for protein quantification in donated human milk
title_sort Comparative analysis of different methods for protein quantification in donated human milk
dc.creator.none.fl_str_mv Navarro Tapia, Elisabet
Herranz Barbero, Ana
Marquina, Maribel
Borrás-Novell, Cristina
Pleguezuelos, Vanessa
Candel Vila, Rafael
García Algar, Óscar
Andreu Fernández, Vicente
author Navarro Tapia, Elisabet
author_facet Navarro Tapia, Elisabet
Herranz Barbero, Ana
Marquina, Maribel
Borrás-Novell, Cristina
Pleguezuelos, Vanessa
Candel Vila, Rafael
García Algar, Óscar
Andreu Fernández, Vicente
author_role author
author2 Herranz Barbero, Ana
Marquina, Maribel
Borrás-Novell, Cristina
Pleguezuelos, Vanessa
Candel Vila, Rafael
García Algar, Óscar
Andreu Fernández, Vicente
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Alimentació dels nodrissons
Llet materna
Proteïnes de la llet
Infants prematurs
Nodrissons
Infants nutrition
Breast milk
Milk proteins
Premature infants
Infants
topic Alimentació dels nodrissons
Llet materna
Proteïnes de la llet
Infants prematurs
Nodrissons
Infants nutrition
Breast milk
Milk proteins
Premature infants
Infants
description Background: Human milk is the best option for feeding newborns, especially premature infants. In the absence of breast milk, milk from a human milk bank can be a suitable alternative. However, the nutritional content of human milk may be insufficient to meet these high requirements and milk fortification is needed. To facilitate the implementation of simpler and faster analyzers in neonatal healthcare facilities, this study focuses on the concordance analysis of two different analyzers, one based on mid-infrared and the other on ultrasound, in comparison to the Bradford method for determining protein concentration in human milk. Methods: Mature milk samples from donor mothers were collected and pasteurized at the Human Milk Bank of Barcelona and protein quantification was performed using mid-infrared (MIRIS-HMA), ultrasound (MilkoScope Julie27), and the classical Bradford reference methods. The intraclass correlation coefficient (ICC) with 95% confidence interval and Bland-Altman plots were used to assess the agreement between methods. Results: The mean protein concentration of 142 milk samples calculated using MIRIS-HMA, MilkoScope, and the Bradford assay were 1.38, 1.15, and 1.19 g/100 ml, respectively. The ICC was 0.70 for MIRIS-HMA vs. Bradford and 0.37 for MilkoScope vs. Bradford. Conclusion: MIRIS-HMA obtained a better agreement with the Bradford technique and is a promising method for developing new devices based on MIR transmission spectroscopy principles. This study confirms how MIRIS-HMA can be used to accurately calculate the protein concentration of human milk.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/215908
url https://hdl.handle.net/2445/215908
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3389/fped.2024.1436885
Frontiers in Pediatrics, 2024, vol. 12, 1436885
https://doi.org/10.3389/fped.2024.1436885
dc.rights.none.fl_str_mv cc-by (c) Elisabet Navarro-Tapia et al., 2024
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Elisabet Navarro-Tapia et al., 2024
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Articles publicats en revistes (BCNatal Fetal Medicine Research Center)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869413487123365888
score 15,811543

Similares en LA Referencia