Metabarcoding of the 18S rRNA gene to uncover new molecular biodiversity in Metazoa and unicellular Opisthokonta
One of the main evolutionary transitions is the origin of animal and fungal multicellularity from single-cell ancestors. To address this change, it is necessary to study the extant unicellular relatives of animals (Metazoa) and fungi, all of them forming the Opisthokonta clade. Recent data have show...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2019 |
| País: | España |
| Institución: | CBUC, CESCA |
| Repositorio: | TDR. Tesis Doctorales en Red |
| OAI Identifier: | oai:www.tdx.cat:10803/667270 |
| Acceso en línea: | http://hdl.handle.net/10803/667270 |
| Access Level: | acceso abierto |
| Palabra clave: | Molecular biodeversity Opisthokonta Metabarcoding Prosits Animals Diversidad molecular Protistas Animales 575 |
| Sumario: | One of the main evolutionary transitions is the origin of animal and fungal multicellularity from single-cell ancestors. To address this change, it is necessary to study the extant unicellular relatives of animals (Metazoa) and fungi, all of them forming the Opisthokonta clade. Recent data have shown that adding more taxa to the Opisthokonta tree can dramatically change our view on this transition. However, the real diversity of these organisms is mostly unknown. Therefore, the main goal of this Ph.D. was to analyse the molecular diversity of both microbial opisthokonts as well as animals, searching for genetic novelty in the form of potential novel clades, as well as trying to better understand their ecology. Using Next-Generation Sequencing data, mainly metabarcoding based on the universal marker 18S rRNA gene, I have performed phylogenetic and ecological inferences, together with graph analysis, to unravel new undescribed diversity of Opisthokonta organisms. |
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