Partially coherent optical diffraction tomography for label-free quantitative microscopy

In this work we present a tomographic technique for label-free quantitative microscopy that exploits the benefits of the partially coherent illumination. In contrast to conventional holographic techniques based on the coherent optical diffraction tomography (C-ODT), our approach uses an inexpensive...

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Detalhes bibliográficos
Autores: Soto Rueda, Juan Manuel, Rodrigo Martín-Romo, José Augusto, Alieva Krasheninnikova, Tatiana
Formato: artículo
Fecha de publicación:2017
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/18657
Acesso em linha:https://hdl.handle.net/20.500.14352/18657
Access Level:acceso abierto
Palavra-chave:535
Light microscopy
Óptica (Física)
2209.19 Óptica Física
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oai_identifier_str oai:docta.ucm.es:20.500.14352/18657
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spelling Partially coherent optical diffraction tomography for label-free quantitative microscopySoto Rueda, Juan ManuelRodrigo Martín-Romo, José AugustoAlieva Krasheninnikova, Tatiana535Light microscopyÓptica (Física)2209.19 Óptica FísicaIn this work we present a tomographic technique for label-free quantitative microscopy that exploits the benefits of the partially coherent illumination. In contrast to conventional holographic techniques based on the coherent optical diffraction tomography (C-ODT), our approach uses an inexpensive non-interferometric setup comprising an electrically tunable lens and low spatially coherent illumination. This setup provides the refocusing needed for axially scan the sample and eventually producing a 3D stack of intensity images. Moreover, the partially coherent illumination avoids speckle noise, which plagues the C-ODT imaging methods. This technique has been developed for optically weak and almost non-absorbing objects that meet the conditions of the first Born approximation. We experimentally demonstrate the capability of the proposed technique by recovering the 3D refractive index distribution of biological samples such as blood cells and diatoms. The results are similar to those obtained by the C-ODT but using a simplified reconstruction process and a refocusing module that can be easily included in commercial widefield microscopes.IEEEUniversidad Complutense de Madrid20172017-09-1820172017-09-18journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/18657reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/186572026-06-02T12:44:21Z
dc.title.none.fl_str_mv Partially coherent optical diffraction tomography for label-free quantitative microscopy
title Partially coherent optical diffraction tomography for label-free quantitative microscopy
spellingShingle Partially coherent optical diffraction tomography for label-free quantitative microscopy
Soto Rueda, Juan Manuel
535
Light microscopy
Óptica (Física)
2209.19 Óptica Física
title_short Partially coherent optical diffraction tomography for label-free quantitative microscopy
title_full Partially coherent optical diffraction tomography for label-free quantitative microscopy
title_fullStr Partially coherent optical diffraction tomography for label-free quantitative microscopy
title_full_unstemmed Partially coherent optical diffraction tomography for label-free quantitative microscopy
title_sort Partially coherent optical diffraction tomography for label-free quantitative microscopy
dc.creator.none.fl_str_mv Soto Rueda, Juan Manuel
Rodrigo Martín-Romo, José Augusto
Alieva Krasheninnikova, Tatiana
author Soto Rueda, Juan Manuel
author_facet Soto Rueda, Juan Manuel
Rodrigo Martín-Romo, José Augusto
Alieva Krasheninnikova, Tatiana
author_role author
author2 Rodrigo Martín-Romo, José Augusto
Alieva Krasheninnikova, Tatiana
author2_role author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 535
Light microscopy
Óptica (Física)
2209.19 Óptica Física
topic 535
Light microscopy
Óptica (Física)
2209.19 Óptica Física
description In this work we present a tomographic technique for label-free quantitative microscopy that exploits the benefits of the partially coherent illumination. In contrast to conventional holographic techniques based on the coherent optical diffraction tomography (C-ODT), our approach uses an inexpensive non-interferometric setup comprising an electrically tunable lens and low spatially coherent illumination. This setup provides the refocusing needed for axially scan the sample and eventually producing a 3D stack of intensity images. Moreover, the partially coherent illumination avoids speckle noise, which plagues the C-ODT imaging methods. This technique has been developed for optically weak and almost non-absorbing objects that meet the conditions of the first Born approximation. We experimentally demonstrate the capability of the proposed technique by recovering the 3D refractive index distribution of biological samples such as blood cells and diatoms. The results are similar to those obtained by the C-ODT but using a simplified reconstruction process and a refocusing module that can be easily included in commercial widefield microscopes.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-09-18
2017
2017-09-18
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/18657
url https://hdl.handle.net/20.500.14352/18657
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv IEEE
publisher.none.fl_str_mv IEEE
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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