An expanded agar-based screening method for azole-resistant Aspergillus fumigatus.

Antifungal susceptibility testing is an essential tool for guiding antifungal therapy. Reference methods are complex and usually only available in specialised laboratories. We have designed an expanded agar-based screening method for the detection of azole-resistant Aspergillus fumigatus isolates. N...

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Detalles Bibliográficos
Autores: Lucio, Jose, Gonzalez-Jimenez, Irene, Garcia-Rubio, Rocio, Cuetara, Maria Soledad, Mellado, Emilia
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/26018
Acceso en línea:https://hdl.handle.net/20.500.12105/26018
Access Level:acceso abierto
Palabra clave:Aspergillus fumigatus
Cyp51A mutations
Azole resistance
Screening method
Surveillance studies
Agar
Antifungal Agents
Azoles
Drug Resistance, Fungal
Fungal Proteins
Microbial Sensitivity Tests
Voriconazole
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repository_id_str
spelling An expanded agar-based screening method for azole-resistant Aspergillus fumigatus.Lucio, JoseGonzalez-Jimenez, IreneGarcia-Rubio, RocioCuetara, Maria SoledadMellado, EmiliaAspergillus fumigatusCyp51A mutationsAzole resistanceScreening methodSurveillance studiesAgarAntifungal AgentsAspergillus fumigatusAzolesDrug Resistance, FungalFungal ProteinsMicrobial Sensitivity TestsVoriconazoleAntifungal susceptibility testing is an essential tool for guiding antifungal therapy. Reference methods are complex and usually only available in specialised laboratories. We have designed an expanded agar-based screening method for the detection of azole-resistant Aspergillus fumigatus isolates. Normally, identification of resistance mechanisms is obtained only after sequencing the cyp51A gene and promoter. However, our screening method provides azole resistance detection and presumptive resistance mechanisms identification. A previous agar-based method consisting of four wells containing voriconazole, itraconazole, posaconazole and a growth control, detected azole resistance to clinical azoles. Here, we have modified the concentrations of voriconazole and posaconazole to adapt to the updated EUCAST breakpoints against A. fumigatus. We have also expanded the method to include environmental azoles to assess azole resistance and the azole resistance mechanism involved. We used a collection of A. fumigatus including 54 azole-resistant isolates with Cyp51A modifications (G54, M220, G448S, TR53 , TR34 /L98H, TR46 /Y121F/T289A, TR34 /L98H/S297T/F495I), and 50 azole susceptible isolates with wild-type Cyp51A. The screening method detects azole-resistant A. fumigatus isolates when there is growth in any of the azole-containing wells after 48h. The growth pattern in the seven azoles tested helps determine the underlying azole resistance mechanism. This approach is designed for surveillance screening of A. fumigatus azole-resistant isolates and can be useful for the clinical management of patients prior to antifungal susceptibility testing confirmation.WileyInstituto de Salud Carlos IIIPlan Nacional de I+D+i (España)Ministerio de Economía, Industria y Competitividad (España)RETICS-Investigación en Patología Infecciosa (REIPI-ISCIII) (España)Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)20252025-01-1420222022-02-0120222022-02-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.12105/26018reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)InglésengES FISPI18CIII 00045ES RD16 CIII 0003ES F17CIII 00037open accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/260182026-06-12T12:43:37Z
dc.title.none.fl_str_mv An expanded agar-based screening method for azole-resistant Aspergillus fumigatus.
title An expanded agar-based screening method for azole-resistant Aspergillus fumigatus.
spellingShingle An expanded agar-based screening method for azole-resistant Aspergillus fumigatus.
Lucio, Jose
Aspergillus fumigatus
Cyp51A mutations
Azole resistance
Screening method
Surveillance studies
Agar
Antifungal Agents
Aspergillus fumigatus
Azoles
Drug Resistance, Fungal
Fungal Proteins
Microbial Sensitivity Tests
Voriconazole
title_short An expanded agar-based screening method for azole-resistant Aspergillus fumigatus.
title_full An expanded agar-based screening method for azole-resistant Aspergillus fumigatus.
title_fullStr An expanded agar-based screening method for azole-resistant Aspergillus fumigatus.
title_full_unstemmed An expanded agar-based screening method for azole-resistant Aspergillus fumigatus.
title_sort An expanded agar-based screening method for azole-resistant Aspergillus fumigatus.
dc.creator.none.fl_str_mv Lucio, Jose
Gonzalez-Jimenez, Irene
Garcia-Rubio, Rocio
Cuetara, Maria Soledad
Mellado, Emilia
author Lucio, Jose
author_facet Lucio, Jose
Gonzalez-Jimenez, Irene
Garcia-Rubio, Rocio
Cuetara, Maria Soledad
Mellado, Emilia
author_role author
author2 Gonzalez-Jimenez, Irene
Garcia-Rubio, Rocio
Cuetara, Maria Soledad
Mellado, Emilia
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
Plan Nacional de I+D+i (España)
Ministerio de Economía, Industria y Competitividad (España)
RETICS-Investigación en Patología Infecciosa (REIPI-ISCIII) (España)
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
dc.subject.none.fl_str_mv Aspergillus fumigatus
Cyp51A mutations
Azole resistance
Screening method
Surveillance studies
Agar
Antifungal Agents
Aspergillus fumigatus
Azoles
Drug Resistance, Fungal
Fungal Proteins
Microbial Sensitivity Tests
Voriconazole
topic Aspergillus fumigatus
Cyp51A mutations
Azole resistance
Screening method
Surveillance studies
Agar
Antifungal Agents
Aspergillus fumigatus
Azoles
Drug Resistance, Fungal
Fungal Proteins
Microbial Sensitivity Tests
Voriconazole
description Antifungal susceptibility testing is an essential tool for guiding antifungal therapy. Reference methods are complex and usually only available in specialised laboratories. We have designed an expanded agar-based screening method for the detection of azole-resistant Aspergillus fumigatus isolates. Normally, identification of resistance mechanisms is obtained only after sequencing the cyp51A gene and promoter. However, our screening method provides azole resistance detection and presumptive resistance mechanisms identification. A previous agar-based method consisting of four wells containing voriconazole, itraconazole, posaconazole and a growth control, detected azole resistance to clinical azoles. Here, we have modified the concentrations of voriconazole and posaconazole to adapt to the updated EUCAST breakpoints against A. fumigatus. We have also expanded the method to include environmental azoles to assess azole resistance and the azole resistance mechanism involved. We used a collection of A. fumigatus including 54 azole-resistant isolates with Cyp51A modifications (G54, M220, G448S, TR53 , TR34 /L98H, TR46 /Y121F/T289A, TR34 /L98H/S297T/F495I), and 50 azole susceptible isolates with wild-type Cyp51A. The screening method detects azole-resistant A. fumigatus isolates when there is growth in any of the azole-containing wells after 48h. The growth pattern in the seven azoles tested helps determine the underlying azole resistance mechanism. This approach is designed for surveillance screening of A. fumigatus azole-resistant isolates and can be useful for the clinical management of patients prior to antifungal susceptibility testing confirmation.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-02-01
2022
2022-02-01
2025
2025-01-14
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.12105/26018
url https://hdl.handle.net/20.500.12105/26018
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv ES FISPI18CIII 00045
ES RD16 CIII 0003
ES F17CIII 00037
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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