The F1F0-ATPase of Bifidobacterium animalis is involved in bile tolerance

Adaptation and tolerance to bile stress are important factors for the survival of bifidobacteria in the intestinal tract. Bifidobacterium animalis is a probiotic microorganism which has been largely applied in fermented dairy foods due to its technological properties and its health-promoting effects...

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Authors: Sánchez García, Borja, González de los Reyes-Gavilán, Clara, Margolles Barros, Abelardo
Format: article
Publication Date:2006
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/6873
Online Access:http://hdl.handle.net/10261/6873
Access Level:Open access
Keyword:Bifidobacteria
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spelling The F1F0-ATPase of Bifidobacterium animalis is involved in bile toleranceSánchez García, BorjaGonzález de los Reyes-Gavilán, ClaraMargolles Barros, AbelardoBifidobacteriaAdaptation and tolerance to bile stress are important factors for the survival of bifidobacteria in the intestinal tract. Bifidobacterium animalis is a probiotic microorganism which has been largely applied in fermented dairy foods due to its technological properties and its health-promoting effects for humans. The effect of the presence of bile on the activity and expression of F1F0-ATPase, the pool of ATP and the intracellular pH of B. animalis IPLA 4549 and its mutant with acquired resistance to bile B. animalis 4549dOx was determined. The bile-resistant mutant tolerated the acid pH better than its parent strain. Bile induced the expression of the F1F0-ATPase and increased the membrane-bound H+-ATPase activity, in both parent and mutant strains. In acidic conditions (pH 5.0), the expression and the activity of this enzyme were higher in the mutant than in the parent strain when cells were grown in the absence of bile. Total ATP content was higher for the mutant in the absence of bile, whereas the presence of bile induced a decrease of intracellular ATP levels, which was much more pronounced for the parent strain. At pH 4.0, and independently on the presence or absence of bile, the mutant showed a higher intracellular pH than its parent strain. These findings suggest that the bile-adapted B. animalis strain is able to tolerate bile by increasing the intracellular ATP reserve, and by inducing proton pumping by the F1F0-ATPase, therefore tightly regulating the internal pH, and provide a link between the physiological state of the cell and the response to bileThis work was financed by European Union FEDER funds and the Spanish Plan Nacional de I + D (AGL2004-06727-C02-01/ALI). B.S. was the recipient of a FPI predoctoral fellowship from the Spanish Ministerio de Educación y Ciencia.Peer reviewedSociety for Applied MicrobiologyBlackwell PublishingEuropean CommissionComisión Interministerial de Ciencia y Tecnología, CICYT (España)Ministerio de Educación y Ciencia (España)200820082006info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501222479 bytesapplication/pdfhttp://hdl.handle.net/10261/6873reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1111/j.1462-2920.2006.01067.xinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/68732026-05-22T06:33:51Z
dc.title.none.fl_str_mv The F1F0-ATPase of Bifidobacterium animalis is involved in bile tolerance
title The F1F0-ATPase of Bifidobacterium animalis is involved in bile tolerance
spellingShingle The F1F0-ATPase of Bifidobacterium animalis is involved in bile tolerance
Sánchez García, Borja
Bifidobacteria
title_short The F1F0-ATPase of Bifidobacterium animalis is involved in bile tolerance
title_full The F1F0-ATPase of Bifidobacterium animalis is involved in bile tolerance
title_fullStr The F1F0-ATPase of Bifidobacterium animalis is involved in bile tolerance
title_full_unstemmed The F1F0-ATPase of Bifidobacterium animalis is involved in bile tolerance
title_sort The F1F0-ATPase of Bifidobacterium animalis is involved in bile tolerance
dc.creator.none.fl_str_mv Sánchez García, Borja
González de los Reyes-Gavilán, Clara
Margolles Barros, Abelardo
author Sánchez García, Borja
author_facet Sánchez García, Borja
González de los Reyes-Gavilán, Clara
Margolles Barros, Abelardo
author_role author
author2 González de los Reyes-Gavilán, Clara
Margolles Barros, Abelardo
author2_role author
author
dc.contributor.none.fl_str_mv European Commission
Comisión Interministerial de Ciencia y Tecnología, CICYT (España)
Ministerio de Educación y Ciencia (España)
dc.subject.none.fl_str_mv Bifidobacteria
topic Bifidobacteria
description Adaptation and tolerance to bile stress are important factors for the survival of bifidobacteria in the intestinal tract. Bifidobacterium animalis is a probiotic microorganism which has been largely applied in fermented dairy foods due to its technological properties and its health-promoting effects for humans. The effect of the presence of bile on the activity and expression of F1F0-ATPase, the pool of ATP and the intracellular pH of B. animalis IPLA 4549 and its mutant with acquired resistance to bile B. animalis 4549dOx was determined. The bile-resistant mutant tolerated the acid pH better than its parent strain. Bile induced the expression of the F1F0-ATPase and increased the membrane-bound H+-ATPase activity, in both parent and mutant strains. In acidic conditions (pH 5.0), the expression and the activity of this enzyme were higher in the mutant than in the parent strain when cells were grown in the absence of bile. Total ATP content was higher for the mutant in the absence of bile, whereas the presence of bile induced a decrease of intracellular ATP levels, which was much more pronounced for the parent strain. At pH 4.0, and independently on the presence or absence of bile, the mutant showed a higher intracellular pH than its parent strain. These findings suggest that the bile-adapted B. animalis strain is able to tolerate bile by increasing the intracellular ATP reserve, and by inducing proton pumping by the F1F0-ATPase, therefore tightly regulating the internal pH, and provide a link between the physiological state of the cell and the response to bile
publishDate 2006
dc.date.none.fl_str_mv 2006
2008
2008
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/6873
url http://hdl.handle.net/10261/6873
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1111/j.1462-2920.2006.01067.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 222479 bytes
application/pdf
dc.publisher.none.fl_str_mv Society for Applied Microbiology
Blackwell Publishing
publisher.none.fl_str_mv Society for Applied Microbiology
Blackwell Publishing
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
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