Hypooxytocinaemia in obese Zucker rats relates to oxytocin degradation in liver and adipose tissue.

The metabolic action of oxytocin has recently been intensively studied to assess the ability of the peptide to regulate energy homeostasis. Despite the obvious weight-reducing effect of oxytocin observed in experimental studies, plasma oxytocin levels were found to be unchanged or even elevated in h...

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Autores: Gajdosechova, L, Krskova, K, Segarra, A, Spolcova, A, Suski, M, Olszanecki, R, Zorad, S
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Universidad de Jaén
Repositorio:RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
OAI Identifier:oai:ruja.ujaen.es:10953/1888
Acceso en línea:https://joe.bioscientifica.com/view/journals/joe/220/3/333.xml
https://hdl.handle.net/10953/1888
Access Level:acceso abierto
Palabra clave:Endocrinology & Metabolism
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spelling Hypooxytocinaemia in obese Zucker rats relates to oxytocin degradation in liver and adipose tissue.Gajdosechova, LKrskova, KSegarra, ASpolcova, ASuski, MOlszanecki, RZorad, SEndocrinology & MetabolismThe metabolic action of oxytocin has recently been intensively studied to assess the ability of the peptide to regulate energy homeostasis. Despite the obvious weight-reducing effect of oxytocin observed in experimental studies, plasma oxytocin levels were found to be unchanged or even elevated in human obesity. The aim of our study was to evaluate the changes in the oxytocin system in Zucker rats, an animal model closely mirroring morbid obesity in humans. Plasma oxytocin levels were measured in obese Zucker rats and lean controls by enzyme immunoassay after plasma extraction. The expression of oxytocin and oxytocin receptor (OXTR) was assessed at the mRNA and protein levels by quantitative real-time PCR and immunoblotting respectively. Plasma and tissue activity of oxytocinase, the main enzyme involved in oxytocin degradation, were measured by fluorometric assay using an arylamide derivate as the substrate. Obese Zucker rats displayed a marked reduction in plasma oxytocin levels. Elevated liver and adipose tissue oxytocinase activity was noticed in obese Zucker rats. Hypothalamic oxytocin gene expression was not altered by the obese phenotype. OXTR mRNA and protein levels were upregulated in the adipose tissue of obese animals in contrast to the reduced OXTR protein levels in skeletal muscle. Our results show that obesity is associated with reduced plasma oxytocin due to increased peptide degradation by liver and adipose tissue rather than changes in hormone synthesis. This study highlights the importance of the oxytocin system in the pathogenesis of obesity and suggests oxytocinase inhibition as a candidate approach in the therapy of obesity.This work was supported by the grants APVV 0028-10 and CE NOREG (Slovakia) and the National Science Centre 2011/01M/NZ04/03752 (Poland).BIOSCIENTIFICA LTDSTARLING HOUSE, 1600 BRISTOL PARKWAY N, BRISTOL BS34 8YU, ENGLAND202420242014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://joe.bioscientifica.com/view/journals/joe/220/3/333.xmlhttps://hdl.handle.net/10953/1888reponame:RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaéninstname:Universidad de JaénInglésJournal of endocrinologyAtribución-NoComercial-SinDerivadas 3.0 Españahttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:ruja.ujaen.es:10953/18882026-06-24T12:41:07Z
dc.title.none.fl_str_mv Hypooxytocinaemia in obese Zucker rats relates to oxytocin degradation in liver and adipose tissue.
title Hypooxytocinaemia in obese Zucker rats relates to oxytocin degradation in liver and adipose tissue.
spellingShingle Hypooxytocinaemia in obese Zucker rats relates to oxytocin degradation in liver and adipose tissue.
Gajdosechova, L
Endocrinology & Metabolism
title_short Hypooxytocinaemia in obese Zucker rats relates to oxytocin degradation in liver and adipose tissue.
title_full Hypooxytocinaemia in obese Zucker rats relates to oxytocin degradation in liver and adipose tissue.
title_fullStr Hypooxytocinaemia in obese Zucker rats relates to oxytocin degradation in liver and adipose tissue.
title_full_unstemmed Hypooxytocinaemia in obese Zucker rats relates to oxytocin degradation in liver and adipose tissue.
title_sort Hypooxytocinaemia in obese Zucker rats relates to oxytocin degradation in liver and adipose tissue.
dc.creator.none.fl_str_mv Gajdosechova, L
Krskova, K
Segarra, A
Spolcova, A
Suski, M
Olszanecki, R
Zorad, S
author Gajdosechova, L
author_facet Gajdosechova, L
Krskova, K
Segarra, A
Spolcova, A
Suski, M
Olszanecki, R
Zorad, S
author_role author
author2 Krskova, K
Segarra, A
Spolcova, A
Suski, M
Olszanecki, R
Zorad, S
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Endocrinology & Metabolism
topic Endocrinology & Metabolism
description The metabolic action of oxytocin has recently been intensively studied to assess the ability of the peptide to regulate energy homeostasis. Despite the obvious weight-reducing effect of oxytocin observed in experimental studies, plasma oxytocin levels were found to be unchanged or even elevated in human obesity. The aim of our study was to evaluate the changes in the oxytocin system in Zucker rats, an animal model closely mirroring morbid obesity in humans. Plasma oxytocin levels were measured in obese Zucker rats and lean controls by enzyme immunoassay after plasma extraction. The expression of oxytocin and oxytocin receptor (OXTR) was assessed at the mRNA and protein levels by quantitative real-time PCR and immunoblotting respectively. Plasma and tissue activity of oxytocinase, the main enzyme involved in oxytocin degradation, were measured by fluorometric assay using an arylamide derivate as the substrate. Obese Zucker rats displayed a marked reduction in plasma oxytocin levels. Elevated liver and adipose tissue oxytocinase activity was noticed in obese Zucker rats. Hypothalamic oxytocin gene expression was not altered by the obese phenotype. OXTR mRNA and protein levels were upregulated in the adipose tissue of obese animals in contrast to the reduced OXTR protein levels in skeletal muscle. Our results show that obesity is associated with reduced plasma oxytocin due to increased peptide degradation by liver and adipose tissue rather than changes in hormone synthesis. This study highlights the importance of the oxytocin system in the pathogenesis of obesity and suggests oxytocinase inhibition as a candidate approach in the therapy of obesity.
publishDate 2014
dc.date.none.fl_str_mv 2014
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://joe.bioscientifica.com/view/journals/joe/220/3/333.xml
https://hdl.handle.net/10953/1888
url https://joe.bioscientifica.com/view/journals/joe/220/3/333.xml
https://hdl.handle.net/10953/1888
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Journal of endocrinology
dc.rights.none.fl_str_mv Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BIOSCIENTIFICA LTDSTARLING HOUSE, 1600 BRISTOL PARKWAY N, BRISTOL BS34 8YU, ENGLAND
publisher.none.fl_str_mv BIOSCIENTIFICA LTDSTARLING HOUSE, 1600 BRISTOL PARKWAY N, BRISTOL BS34 8YU, ENGLAND
dc.source.none.fl_str_mv reponame:RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
instname:Universidad de Jaén
instname_str Universidad de Jaén
reponame_str RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
collection RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
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