AQP1 and AQP4 Contribution to Cerebrospinal Fluid Homeostasis

Aquaporin 1 (AQP1), expressed in epithelial cells of the choroid plexus, and aquaporin 4 (AQP4) present in ependymal cells and glia limitants have been proposed to play a significant role in cerebrospinal fluid (CSF) production and homeostasis. However, the specific contribution of each water channe...

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Autores: Trillo Contreras, José Luis, Toledo Aral, Juan José, Echevarría Irusta, Miriam, Villadiego Luque, Francisco Javier
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2019
País:España
Recursos:Universidad de Sevilla (US)
Repositório:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/89042
Acesso em linha:https://hdl.handle.net/11441/89042
https://doi.org/10.3390/cells8020197
Access Level:Acceso aberto
Palavra-chave:Aquaporins
AQP1
AQP4
Cerebrospinal fluid
Astrocytes
Choroid plexus
Cerebral ventricles
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spelling AQP1 and AQP4 Contribution to Cerebrospinal Fluid HomeostasisTrillo Contreras, José LuisToledo Aral, Juan JoséEchevarría Irusta, MiriamVilladiego Luque, Francisco JavierAquaporinsAQP1AQP4Cerebrospinal fluidAstrocytesChoroid plexusCerebral ventriclesAquaporin 1 (AQP1), expressed in epithelial cells of the choroid plexus, and aquaporin 4 (AQP4) present in ependymal cells and glia limitants have been proposed to play a significant role in cerebrospinal fluid (CSF) production and homeostasis. However, the specific contribution of each water channel to these functions remains unknown, being a subject of debate during the last years. Here, we analyzed in detail how AQP1 and AQP4 participate in different aspects of the CSF homeostasis such as the load and drainage of ventricles, and further explored if these proteins play a role in the ventricular compliance. To do that, we carried out records of intraventricular pressure and CSF outflow, and evaluated ventricular volume by magnetic resonance imaging in AQP1−/−, AQP4−/−, double AQP1−/−-AQP4−/− knock out and wild type mice controls. The analysis performed clearly showed that both AQPs have a significant participation in the CSF production, and additionally revealed that the double AQP1-AQP4 mutation alters the CSF drainage and the ventricular compliance. The data reported here indicate a significant extra-choroidal CSF formation mediated by AQP4, supporting the idea of an important and constant CSF production/absorption process, sustained by efflux/influx of water between brain capillaries and interstitial fluid. Moreover, our results suggest the participation of AQPs in structural functions also related with CSF homeostasis such as the distensibility capacity of the ventricular system.Ministerio de Economía y Competitividad PI12/01882Ministerio de Economía y Competitividad PI16/00493Instituto de Salud Carlos IIIEuropean Regional Development Fund (FEDER)Junta de AndalucíaMDPIFisiología Médica y BiofísicaCTS517: Fisiologia MolecularCTS1047: Fisiopatología Asociada a las Acuaporinas2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/89042https://doi.org/10.3390/cells8020197reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésCells, 8 (2), 1-12.info:eu-repo/semantics/openAccessoai:idus.us.es:11441/890422026-06-17T12:51:07Z
dc.title.none.fl_str_mv AQP1 and AQP4 Contribution to Cerebrospinal Fluid Homeostasis
title AQP1 and AQP4 Contribution to Cerebrospinal Fluid Homeostasis
spellingShingle AQP1 and AQP4 Contribution to Cerebrospinal Fluid Homeostasis
Trillo Contreras, José Luis
Aquaporins
AQP1
AQP4
Cerebrospinal fluid
Astrocytes
Choroid plexus
Cerebral ventricles
title_short AQP1 and AQP4 Contribution to Cerebrospinal Fluid Homeostasis
title_full AQP1 and AQP4 Contribution to Cerebrospinal Fluid Homeostasis
title_fullStr AQP1 and AQP4 Contribution to Cerebrospinal Fluid Homeostasis
title_full_unstemmed AQP1 and AQP4 Contribution to Cerebrospinal Fluid Homeostasis
title_sort AQP1 and AQP4 Contribution to Cerebrospinal Fluid Homeostasis
dc.creator.none.fl_str_mv Trillo Contreras, José Luis
Toledo Aral, Juan José
Echevarría Irusta, Miriam
Villadiego Luque, Francisco Javier
author Trillo Contreras, José Luis
author_facet Trillo Contreras, José Luis
Toledo Aral, Juan José
Echevarría Irusta, Miriam
Villadiego Luque, Francisco Javier
author_role author
author2 Toledo Aral, Juan José
Echevarría Irusta, Miriam
Villadiego Luque, Francisco Javier
author2_role author
author
author
dc.contributor.none.fl_str_mv Fisiología Médica y Biofísica
CTS517: Fisiologia Molecular
CTS1047: Fisiopatología Asociada a las Acuaporinas
dc.subject.none.fl_str_mv Aquaporins
AQP1
AQP4
Cerebrospinal fluid
Astrocytes
Choroid plexus
Cerebral ventricles
topic Aquaporins
AQP1
AQP4
Cerebrospinal fluid
Astrocytes
Choroid plexus
Cerebral ventricles
description Aquaporin 1 (AQP1), expressed in epithelial cells of the choroid plexus, and aquaporin 4 (AQP4) present in ependymal cells and glia limitants have been proposed to play a significant role in cerebrospinal fluid (CSF) production and homeostasis. However, the specific contribution of each water channel to these functions remains unknown, being a subject of debate during the last years. Here, we analyzed in detail how AQP1 and AQP4 participate in different aspects of the CSF homeostasis such as the load and drainage of ventricles, and further explored if these proteins play a role in the ventricular compliance. To do that, we carried out records of intraventricular pressure and CSF outflow, and evaluated ventricular volume by magnetic resonance imaging in AQP1−/−, AQP4−/−, double AQP1−/−-AQP4−/− knock out and wild type mice controls. The analysis performed clearly showed that both AQPs have a significant participation in the CSF production, and additionally revealed that the double AQP1-AQP4 mutation alters the CSF drainage and the ventricular compliance. The data reported here indicate a significant extra-choroidal CSF formation mediated by AQP4, supporting the idea of an important and constant CSF production/absorption process, sustained by efflux/influx of water between brain capillaries and interstitial fluid. Moreover, our results suggest the participation of AQPs in structural functions also related with CSF homeostasis such as the distensibility capacity of the ventricular system.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/89042
https://doi.org/10.3390/cells8020197
url https://hdl.handle.net/11441/89042
https://doi.org/10.3390/cells8020197
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Cells, 8 (2), 1-12.
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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