Penicillin Acylase from Streptomyces lavendulae and Aculeacin A Acylase from Actinoplanes utahensis: Two Versatile Enzymes as Useful Tools for Quorum Quenching Processes

Many Gram-negative bacteria produce N-acyl-homoserine lactones (AHLs), quorum sensing (QS) molecules that can be enzymatically inactivated by quorum quenching (QQ) processes; this approach is considered an emerging antimicrobial alternative. In this study, kinetic parameters of several AHLs hydrolyz...

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Detalles Bibliográficos
Autores: Velasco Bucheli, Rodrigo, Hormigo, Daniel, Fernández-Lucas, Jesús, Torres Ayuso, Pedro, Alfaro Ureña, Yohana, Saborido Modia, Ana Isolina, Serrano Aguirre, Lara, García, José Luis, Ramón, Fernando, Acebal Sarabia, Carmen, Santos de la Sen, Antonio, Arroyo Sánchez, Miguel, De La Mata Riesco, Mª Isabel
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/7938
Acceso en línea:https://hdl.handle.net/20.500.14352/7938
Access Level:acceso abierto
Palabra clave:577.15
579.87
615.33
Penicillin acylase
Aculeacin acylase
N-acyl-homoserine lactone acylases
Quorum quenching
Biofouling Penicillin acylase
Biofouling
Bioquímica (Biología)
Microbiología (Biología)
2302 Bioquímica
2414 Microbiología
Descripción
Sumario:Many Gram-negative bacteria produce N-acyl-homoserine lactones (AHLs), quorum sensing (QS) molecules that can be enzymatically inactivated by quorum quenching (QQ) processes; this approach is considered an emerging antimicrobial alternative. In this study, kinetic parameters of several AHLs hydrolyzed by penicillin acylase from Streptomyces lavendulae (SlPA) and aculeacin A acylase from Actinoplanes utahensis (AuAAC) have been determined. Both enzymes catalyze efficiently the amide bond hydrolysis in AHLs with different acyl chain moieties (with or without 3-oxo modification) and exhibit a clear preference for AHLs with long acyl chains (C12-HSL > C14-HSL > C10-HSL > C8-HSL for SlPA, whereas C14-HSL > C12-HSL > C10-HSL > C8-HSL for AuAAC). Involvement of SlPA and AuAAC in QQ processes was demonstrated by Chromobacterium violaceum CV026-based bioassays and inhibition of biofilm formation by Pseudomonas aeruginosa, a process controlled by QS molecules, suggesting the application of these multifunctional enzymes as quorum quenching agents, this being the first time that quorum quenching activity was shown by an aculeacin A acylase. In addition, a phylogenetic study suggests that SlPA and AuAAC could be part of a new family of actinomycete acylases, with a preference for substrates with long aliphatic acyl chains, and likely involved in QQ processes.

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