The Citrus ABA signalosome: identification and transcriptional regulation during sweet orange fruit ripening and leaf dehydration

The abscisic acid (ABA) signalling core in plants include the cytosolic ABA receptors (PYR/PYL/RCARs), the clade-A type 2C protein phosphatases (PP2CAs), and the subclass III SNF1-related protein kinases 2 (SnRK2s). The aim of this work was to identify these ABA perception system components in sweet...

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Detalles Bibliográficos
Autores: Romero, Paco, Lafuente, María Teresa, Rodrigo, María Jesús
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/376020
Acceso en línea:http://hdl.handle.net/10261/376020
https://api.elsevier.com/content/abstract/scopus_id/84865519188
Access Level:acceso abierto
Palabra clave:Abscisic acid (ABA)
Citrus
Fruit ripening
Gene expression
Leaf dehydration
PP2CA
PYR/PYL/RCAR
gene expression
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repository_id_str
spelling The Citrus ABA signalosome: identification and transcriptional regulation during sweet orange fruit ripening and leaf dehydrationRomero, PacoLafuente, María TeresaRodrigo, María JesúsAbscisic acid (ABA)CitrusFruit ripeningGene expressionLeaf dehydrationPP2CAPYR/PYL/RCARCitrusgene expressionThe abscisic acid (ABA) signalling core in plants include the cytosolic ABA receptors (PYR/PYL/RCARs), the clade-A type 2C protein phosphatases (PP2CAs), and the subclass III SNF1-related protein kinases 2 (SnRK2s). The aim of this work was to identify these ABA perception system components in sweet orange and to determine the influence of endogenous ABA on their transcriptional regulation during fruit development and ripening, taking advantage of the comparative analysis between a wild-type and a fruit-specific ABA-deficient mutant. Transcriptional changes in the ABA signalosome during leaf dehydration were also studied. Six PYR/PYL/RCAR, five PP2CA, and two subclass III SnRK2 genes, homologous to those of Arabidopsis, were identified in the Citrus genome. The high degree of homology and conserved motifs for protein folding and for functional activity suggested that these Citrus proteins are bona fide core elements of ABA perception in orange. Opposite expression patterns of CsPYL4 and CsPYL5 and ABA accumulation were found during ripening, although there were few differences between varieties. In contrast, changes in expression of CsPP2CA genes during ripening paralleled those of ABA content and agreeed with the relevant differences between wild-type and mutant fruit transcript accumulation. CsSnRK2 gene expression continuously decreased with ripening and no remarkable differences were found between cultivars. Overall, dehydration had a minor effect on CsPYR/PYL/RCAR and CsSnRK2 expression in vegetative tissue, whereas CsABI1, CsAHG1, and CsAHG3 were highly induced by water stress. The global results suggest that responsiveness to ABA changes during citrus fruit ripening, and leaf dehydration was higher in the CsPP2CA gene negative regulators than in the other ABA signalosome components.This work was supported by the Spanish Ministry of Science and Technology (Research Grants AGL2006-09496, AGL2009-11969, and AGL2009-11558) and by the Generalitat Valenciana (PROMETEO/2010/010). PR was the recipient of a fellowship from the Spanish Ministry of Science and Technology.Peer reviewedOxford University PressMinisterio de Ciencia y Tecnología (España)Generalitat ValencianaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252012info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/376020https://api.elsevier.com/content/abstract/scopus_id/84865519188reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MEC//AGL2006-09496info:eu-repo/grantAgreement/MICINN//AGL2009-11969info:eu-repo/grantAgreement/MICINN//AGL2009-11558Journal of experimental botanyThe underlying dataset has been published as supplementary material of the article in the publisher platform at http://dx.doi.org/10.1093/jxb/ers168http://dx.doi.org/10.1093/jxb/ers168Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3760202026-05-22T06:33:51Z
dc.title.none.fl_str_mv The Citrus ABA signalosome: identification and transcriptional regulation during sweet orange fruit ripening and leaf dehydration
title The Citrus ABA signalosome: identification and transcriptional regulation during sweet orange fruit ripening and leaf dehydration
spellingShingle The Citrus ABA signalosome: identification and transcriptional regulation during sweet orange fruit ripening and leaf dehydration
Romero, Paco
Abscisic acid (ABA)
Citrus
Fruit ripening
Gene expression
Leaf dehydration
PP2CA
PYR/PYL/RCAR
Citrus
gene expression
title_short The Citrus ABA signalosome: identification and transcriptional regulation during sweet orange fruit ripening and leaf dehydration
title_full The Citrus ABA signalosome: identification and transcriptional regulation during sweet orange fruit ripening and leaf dehydration
title_fullStr The Citrus ABA signalosome: identification and transcriptional regulation during sweet orange fruit ripening and leaf dehydration
title_full_unstemmed The Citrus ABA signalosome: identification and transcriptional regulation during sweet orange fruit ripening and leaf dehydration
title_sort The Citrus ABA signalosome: identification and transcriptional regulation during sweet orange fruit ripening and leaf dehydration
dc.creator.none.fl_str_mv Romero, Paco
Lafuente, María Teresa
Rodrigo, María Jesús
author Romero, Paco
author_facet Romero, Paco
Lafuente, María Teresa
Rodrigo, María Jesús
author_role author
author2 Lafuente, María Teresa
Rodrigo, María Jesús
author2_role author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia y Tecnología (España)
Generalitat Valenciana
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Abscisic acid (ABA)
Citrus
Fruit ripening
Gene expression
Leaf dehydration
PP2CA
PYR/PYL/RCAR
Citrus
gene expression
topic Abscisic acid (ABA)
Citrus
Fruit ripening
Gene expression
Leaf dehydration
PP2CA
PYR/PYL/RCAR
Citrus
gene expression
description The abscisic acid (ABA) signalling core in plants include the cytosolic ABA receptors (PYR/PYL/RCARs), the clade-A type 2C protein phosphatases (PP2CAs), and the subclass III SNF1-related protein kinases 2 (SnRK2s). The aim of this work was to identify these ABA perception system components in sweet orange and to determine the influence of endogenous ABA on their transcriptional regulation during fruit development and ripening, taking advantage of the comparative analysis between a wild-type and a fruit-specific ABA-deficient mutant. Transcriptional changes in the ABA signalosome during leaf dehydration were also studied. Six PYR/PYL/RCAR, five PP2CA, and two subclass III SnRK2 genes, homologous to those of Arabidopsis, were identified in the Citrus genome. The high degree of homology and conserved motifs for protein folding and for functional activity suggested that these Citrus proteins are bona fide core elements of ABA perception in orange. Opposite expression patterns of CsPYL4 and CsPYL5 and ABA accumulation were found during ripening, although there were few differences between varieties. In contrast, changes in expression of CsPP2CA genes during ripening paralleled those of ABA content and agreeed with the relevant differences between wild-type and mutant fruit transcript accumulation. CsSnRK2 gene expression continuously decreased with ripening and no remarkable differences were found between cultivars. Overall, dehydration had a minor effect on CsPYR/PYL/RCAR and CsSnRK2 expression in vegetative tissue, whereas CsABI1, CsAHG1, and CsAHG3 were highly induced by water stress. The global results suggest that responsiveness to ABA changes during citrus fruit ripening, and leaf dehydration was higher in the CsPP2CA gene negative regulators than in the other ABA signalosome components.
publishDate 2012
dc.date.none.fl_str_mv 2012
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/376020
https://api.elsevier.com/content/abstract/scopus_id/84865519188
url http://hdl.handle.net/10261/376020
https://api.elsevier.com/content/abstract/scopus_id/84865519188
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MEC//AGL2006-09496
info:eu-repo/grantAgreement/MICINN//AGL2009-11969
info:eu-repo/grantAgreement/MICINN//AGL2009-11558
Journal of experimental botany
The underlying dataset has been published as supplementary material of the article in the publisher platform at http://dx.doi.org/10.1093/jxb/ers168
http://dx.doi.org/10.1093/jxb/ers168
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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