Effect of dietary plant-feedstuffs and protein/carbohydrate ratio on gilthead seabream (Sparus aurata) gut health and functionality

This study investigated, for the first time, the integrated effects of dietary protein source and protein/carbohydrate (P/CH) ratio on gilthead seabream gut histomorphology, microbiota composition, digestive enzymes activity, and immunological and oxidative stress-related gene expressions. Four isol...

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Autores: Basto-Silva, Catarina, García Meilán, Irene, Couto, Ana, Serra, Cláudia R., Enes, Paula, Oliva-Teles, Aires, Capilla Campos, Encarnación, Guerreiro, Inês
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/186297
Acceso en línea:https://hdl.handle.net/2445/186297
Access Level:acceso abierto
Palabra clave:Orada
Microbiota intestinal
Expressió gènica
Estrès oxidatiu
Sparus aurata
Gastrointestinal microbiome
Gene expression
Oxidative stress
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spelling Effect of dietary plant-feedstuffs and protein/carbohydrate ratio on gilthead seabream (Sparus aurata) gut health and functionalityBasto-Silva, CatarinaGarcía Meilán, IreneCouto, AnaSerra, Cláudia R.Enes, PaulaOliva-Teles, AiresCapilla Campos, EncarnaciónGuerreiro, InêsOradaMicrobiota intestinalExpressió gènicaEstrès oxidatiuSparus aurataGastrointestinal microbiomeGene expressionOxidative stressThis study investigated, for the first time, the integrated effects of dietary protein source and protein/carbohydrate (P/CH) ratio on gilthead seabream gut histomorphology, microbiota composition, digestive enzymes activity, and immunological and oxidative stress-related gene expressions. Four isolipidic diets: two fishmeal-based (FM) and two plant feedstuff (PF)-based diets, with P/CH ratios of 50/10 or 40/20 each (FM-P50/CH10; FM-P40/CH20; PF-P50/CH10; PF-P40/CH20), were tested. PF-based diets lead to more histomorphological alterations than FM-based diets. P/CH ratio had no relevant effect on gut histomorphology. Gut mucosa of fish fed PF-based diets presented a higher number of operational taxonomic units, and richness and diversity indices, while the P/CH ratio did not affect those parameters. The α-amylase activity was lower in fish fed with PF-based diets and in fish fed the P40/CH20 diets. Regarding the immune-related genes, only cyclooxygenase-2 was affected, being higher in fish fed the P50/CH10 diets than the P40/CH20 diets. Fish fed the FM-based diets presented higher expression of glutathione reductase and glutathione peroxidase, while fish fed the P50/CH10 diet had higher expression of superoxide dismutase. In conclusion, PF-based diets can compromise gut absorptive and digestive metabolism, but decreasing the dietary P/CH ratio had little effect on the parameters measured.MDPI2022202220222022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion17 p.application/pdfhttps://hdl.handle.net/2445/186297Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3390/fishes7020059Fishes, 2022, vol. 7, num. 2, p. 59https://doi.org/10.3390/fishes7020059cc-by (c) Basto-Silva, Catarina et al., 2022https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1862972026-05-29T05:05:01Z
dc.title.none.fl_str_mv Effect of dietary plant-feedstuffs and protein/carbohydrate ratio on gilthead seabream (Sparus aurata) gut health and functionality
title Effect of dietary plant-feedstuffs and protein/carbohydrate ratio on gilthead seabream (Sparus aurata) gut health and functionality
spellingShingle Effect of dietary plant-feedstuffs and protein/carbohydrate ratio on gilthead seabream (Sparus aurata) gut health and functionality
Basto-Silva, Catarina
Orada
Microbiota intestinal
Expressió gènica
Estrès oxidatiu
Sparus aurata
Gastrointestinal microbiome
Gene expression
Oxidative stress
title_short Effect of dietary plant-feedstuffs and protein/carbohydrate ratio on gilthead seabream (Sparus aurata) gut health and functionality
title_full Effect of dietary plant-feedstuffs and protein/carbohydrate ratio on gilthead seabream (Sparus aurata) gut health and functionality
title_fullStr Effect of dietary plant-feedstuffs and protein/carbohydrate ratio on gilthead seabream (Sparus aurata) gut health and functionality
title_full_unstemmed Effect of dietary plant-feedstuffs and protein/carbohydrate ratio on gilthead seabream (Sparus aurata) gut health and functionality
title_sort Effect of dietary plant-feedstuffs and protein/carbohydrate ratio on gilthead seabream (Sparus aurata) gut health and functionality
dc.creator.none.fl_str_mv Basto-Silva, Catarina
García Meilán, Irene
Couto, Ana
Serra, Cláudia R.
Enes, Paula
Oliva-Teles, Aires
Capilla Campos, Encarnación
Guerreiro, Inês
author Basto-Silva, Catarina
author_facet Basto-Silva, Catarina
García Meilán, Irene
Couto, Ana
Serra, Cláudia R.
Enes, Paula
Oliva-Teles, Aires
Capilla Campos, Encarnación
Guerreiro, Inês
author_role author
author2 García Meilán, Irene
Couto, Ana
Serra, Cláudia R.
Enes, Paula
Oliva-Teles, Aires
Capilla Campos, Encarnación
Guerreiro, Inês
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Orada
Microbiota intestinal
Expressió gènica
Estrès oxidatiu
Sparus aurata
Gastrointestinal microbiome
Gene expression
Oxidative stress
topic Orada
Microbiota intestinal
Expressió gènica
Estrès oxidatiu
Sparus aurata
Gastrointestinal microbiome
Gene expression
Oxidative stress
description This study investigated, for the first time, the integrated effects of dietary protein source and protein/carbohydrate (P/CH) ratio on gilthead seabream gut histomorphology, microbiota composition, digestive enzymes activity, and immunological and oxidative stress-related gene expressions. Four isolipidic diets: two fishmeal-based (FM) and two plant feedstuff (PF)-based diets, with P/CH ratios of 50/10 or 40/20 each (FM-P50/CH10; FM-P40/CH20; PF-P50/CH10; PF-P40/CH20), were tested. PF-based diets lead to more histomorphological alterations than FM-based diets. P/CH ratio had no relevant effect on gut histomorphology. Gut mucosa of fish fed PF-based diets presented a higher number of operational taxonomic units, and richness and diversity indices, while the P/CH ratio did not affect those parameters. The α-amylase activity was lower in fish fed with PF-based diets and in fish fed the P40/CH20 diets. Regarding the immune-related genes, only cyclooxygenase-2 was affected, being higher in fish fed the P50/CH10 diets than the P40/CH20 diets. Fish fed the FM-based diets presented higher expression of glutathione reductase and glutathione peroxidase, while fish fed the P50/CH10 diet had higher expression of superoxide dismutase. In conclusion, PF-based diets can compromise gut absorptive and digestive metabolism, but decreasing the dietary P/CH ratio had little effect on the parameters measured.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/186297
url https://hdl.handle.net/2445/186297
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/fishes7020059
Fishes, 2022, vol. 7, num. 2, p. 59
https://doi.org/10.3390/fishes7020059
dc.rights.none.fl_str_mv cc-by (c) Basto-Silva, Catarina et al., 2022
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Basto-Silva, Catarina et al., 2022
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 17 p.
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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