New partners identified by mass spectrometry assay reveal functions of NCAM2 in neural cytoskeleton organization

This work was supported by grants from the Spanish MINECO (SAF2016-76340R and PID2019-106764RB-C21) and CIBERNED (ISCIII) to E.S., Spanish MECD (FPU14/02156, Excellence Unit María de Maeztu/Institute of Neurosciences to E.S. and L.P., and BES-2017-080570 to A.O.-G.) and a grant from Secretary of Uni...

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Authors: Parcerisas, Antoni, Ortega-Gascó, Alba, Hernaiz Llorens, Marc, Odena Caballol, Maria Antonia, Ulloa, fausto, de Oliveira, Eliandre, Bosch, Miquel, PUJADAS, LLUÍS, Soriano, Eduardo
Format: article
Publication Date:2021
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.12328/2998
Online Access:http://hdl.handle.net/20.500.12328/2998
https://dx.doi.org/10.3390/ijms22147404
Access Level:Open access
Keyword:NCAM2
Espectrometria de masses
Citoesquelet
Morfogènesi neuronal
MAPA2
CaMKIIα
Neurofilaments
Espectrometría de masas
Citoesqueleto
Morfogénesis neuronal
MAP2
Neurofilamentos
Mass spectrometry
Cytoskeleton
Neuronal morphogenesis
54
57
61
id ES_90b4dd43d483efc35c1753697badbf33
oai_identifier_str oai:recercat.cat:20.500.12328/2998
network_acronym_str ES
network_name_str España
repository_id_str
spelling New partners identified by mass spectrometry assay reveal functions of NCAM2 in neural cytoskeleton organizationParcerisas, AntoniOrtega-Gascó, AlbaHernaiz Llorens, MarcOdena Caballol, Maria AntoniaUlloa, faustode Oliveira, EliandreBosch, MiquelPUJADAS, LLUÍSSoriano, EduardoNCAM2Espectrometria de massesCitoesqueletMorfogènesi neuronalMAPA2CaMKIIαNeurofilamentsNCAM2Espectrometría de masasCitoesqueletoMorfogénesis neuronalMAP2CaMKIIαNeurofilamentosNCAM2Mass spectrometryCytoskeletonNeuronal morphogenesisMAP2CaMKIIαNeurofilaments545761This work was supported by grants from the Spanish MINECO (SAF2016-76340R and PID2019-106764RB-C21) and CIBERNED (ISCIII) to E.S., Spanish MECD (FPU14/02156, Excellence Unit María de Maeztu/Institute of Neurosciences to E.S. and L.P., and BES-2017-080570 to A.O.-G.) and a grant from Secretary of Universities and Research of the Department of Economy and Knowledge of the Generalitat de Catalunya to A.P.Neuronal cell adhesion molecule 2 (NCAM2) is a membrane protein with an important role in the morphological development of neurons. In the cortex and the hippocampus, NCAM2 is essential for proper neuronal differentiation, dendritic and axonal outgrowth and synapse formation. However, little is known about NCAM2 functional mechanisms and its interactive partners during brain development. Here we used mass spectrometry to study the molecular interactome of NCAM2 in the second postnatal week of the mouse cerebral cortex. We found that NCAM2 interacts with >100 proteins involved in numerous processes, including neuronal morphogenesis and synaptogenesis. We validated the most relevant interactors, including Neurofilaments (NEFs), Microtubule-associated protein 2 (MAP2), Calcium/calmodulin kinase II alpha (CaMKIIα), Actin and Nogo. An in silico analysis of the cytosolic tail of the NCAM2.1 isoform revealed specific phosphorylation site motifs with a putative affinity for some of these interactors. Our results expand the knowledge of NCAM2 interactome and confirm the key role of NCAM2 in cytoskeleton organization, neuronal morphogenesis and synaptogenesis. These findings are of interest in explaining the phenotypes observed in different pathologies with alterations in the NCAM2 gene.info:eu-repo/semantics/publishedVersionMDPI2021info:eu-repo/semantics/article20http://hdl.handle.net/20.500.12328/2998https://dx.doi.org/10.3390/ijms22147404reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésInternational Journal of Molecular Sciences22;14This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.12328/29982026-05-29T05:05:01Z
dc.title.none.fl_str_mv New partners identified by mass spectrometry assay reveal functions of NCAM2 in neural cytoskeleton organization
title New partners identified by mass spectrometry assay reveal functions of NCAM2 in neural cytoskeleton organization
spellingShingle New partners identified by mass spectrometry assay reveal functions of NCAM2 in neural cytoskeleton organization
Parcerisas, Antoni
NCAM2
Espectrometria de masses
Citoesquelet
Morfogènesi neuronal
MAPA2
CaMKIIα
Neurofilaments
NCAM2
Espectrometría de masas
Citoesqueleto
Morfogénesis neuronal
MAP2
CaMKIIα
Neurofilamentos
NCAM2
Mass spectrometry
Cytoskeleton
Neuronal morphogenesis
MAP2
CaMKIIα
Neurofilaments
54
57
61
title_short New partners identified by mass spectrometry assay reveal functions of NCAM2 in neural cytoskeleton organization
title_full New partners identified by mass spectrometry assay reveal functions of NCAM2 in neural cytoskeleton organization
title_fullStr New partners identified by mass spectrometry assay reveal functions of NCAM2 in neural cytoskeleton organization
title_full_unstemmed New partners identified by mass spectrometry assay reveal functions of NCAM2 in neural cytoskeleton organization
title_sort New partners identified by mass spectrometry assay reveal functions of NCAM2 in neural cytoskeleton organization
dc.creator.none.fl_str_mv Parcerisas, Antoni
Ortega-Gascó, Alba
Hernaiz Llorens, Marc
Odena Caballol, Maria Antonia
Ulloa, fausto
de Oliveira, Eliandre
Bosch, Miquel
PUJADAS, LLUÍS
Soriano, Eduardo
author Parcerisas, Antoni
author_facet Parcerisas, Antoni
Ortega-Gascó, Alba
Hernaiz Llorens, Marc
Odena Caballol, Maria Antonia
Ulloa, fausto
de Oliveira, Eliandre
Bosch, Miquel
PUJADAS, LLUÍS
Soriano, Eduardo
author_role author
author2 Ortega-Gascó, Alba
Hernaiz Llorens, Marc
Odena Caballol, Maria Antonia
Ulloa, fausto
de Oliveira, Eliandre
Bosch, Miquel
PUJADAS, LLUÍS
Soriano, Eduardo
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv NCAM2
Espectrometria de masses
Citoesquelet
Morfogènesi neuronal
MAPA2
CaMKIIα
Neurofilaments
NCAM2
Espectrometría de masas
Citoesqueleto
Morfogénesis neuronal
MAP2
CaMKIIα
Neurofilamentos
NCAM2
Mass spectrometry
Cytoskeleton
Neuronal morphogenesis
MAP2
CaMKIIα
Neurofilaments
54
57
61
topic NCAM2
Espectrometria de masses
Citoesquelet
Morfogènesi neuronal
MAPA2
CaMKIIα
Neurofilaments
NCAM2
Espectrometría de masas
Citoesqueleto
Morfogénesis neuronal
MAP2
CaMKIIα
Neurofilamentos
NCAM2
Mass spectrometry
Cytoskeleton
Neuronal morphogenesis
MAP2
CaMKIIα
Neurofilaments
54
57
61
description This work was supported by grants from the Spanish MINECO (SAF2016-76340R and PID2019-106764RB-C21) and CIBERNED (ISCIII) to E.S., Spanish MECD (FPU14/02156, Excellence Unit María de Maeztu/Institute of Neurosciences to E.S. and L.P., and BES-2017-080570 to A.O.-G.) and a grant from Secretary of Universities and Research of the Department of Economy and Knowledge of the Generalitat de Catalunya to A.P.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12328/2998
https://dx.doi.org/10.3390/ijms22147404
url http://hdl.handle.net/20.500.12328/2998
https://dx.doi.org/10.3390/ijms22147404
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv International Journal of Molecular Sciences
22;14
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 20
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869413314484764672
score 15,811543

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