Late rDNA condensation ensures timely Cdc14 release and coordination of mitotic exit signaling with nucleolar segregation

The nucleolus plays a pivotal role in multiple key cellular processes. An illustrative example is the regulation of mitotic exit in Saccharomyces cerevisiae through the nucleolar sequestration of the Cdc14 phosphatase. The peculiar structure of the nucleolus, however, has also its drawbacks. The rep...

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Detalhes bibliográficos
Autores: Santos-Velázquez, Ana Isabel, García de Oya, Inés, Manzano-López, Javier, Monje-Casas, Fernando
Tipo de documento: artigo
Data de publicação:2017
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/165562
Acesso em linha:http://hdl.handle.net/10261/165562
Access Level:Acceso aberto
Palavra-chave:Mitotic exit
SnoRNPs
Nucleolus
Condensin
FEAR
Cdc14
MEN
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spelling Late rDNA condensation ensures timely Cdc14 release and coordination of mitotic exit signaling with nucleolar segregationSantos-Velázquez, Ana IsabelGarcía de Oya, InésManzano-López, JavierMonje-Casas, FernandoMitotic exitSnoRNPsNucleolusCondensinFEARCdc14MENThe nucleolus plays a pivotal role in multiple key cellular processes. An illustrative example is the regulation of mitotic exit in Saccharomyces cerevisiae through the nucleolar sequestration of the Cdc14 phosphatase. The peculiar structure of the nucleolus, however, has also its drawbacks. The repetitive nature of the rDNA gives rise to cohesion-independent linkages whose resolution in budding yeast requires the Cdc14-dependent inhibition of rRNA transcription, which facilitates condensin accessibility to this locus. Thus, the rDNA condenses and segregates later than most other yeast genomic regions. Here, we show that defective function of a small nucleolar ribonucleoprotein particle (snoRNP) assembly factor facilitates condensin accessibility to the rDNA and induces nucleolar hyper-condensation. Interestingly, this increased compaction of the nucleolus interferes with the proper release of Cdc14 from this organelle. This observation provides an explanation for the delayed rDNA condensation in budding yeast, which is necessary to efficiently coordinate timely Cdc14 release and mitotic exit with nucleolar compaction and segregation.This work was supported by the Andalusian Regional Government, the European Regional Development Fund (FEDER), and the Spanish Ministry of Economy and Competitiveness (BFU2013-43718-P and BFU2016-76642-P).Peer reviewedPeer ReviewedElsevierJunta de AndalucíaEuropean CommissionMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2018201820172018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/165562reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2013-43718-Pinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2016-76642-PSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1655622026-05-22T06:33:51Z
dc.title.none.fl_str_mv Late rDNA condensation ensures timely Cdc14 release and coordination of mitotic exit signaling with nucleolar segregation
title Late rDNA condensation ensures timely Cdc14 release and coordination of mitotic exit signaling with nucleolar segregation
spellingShingle Late rDNA condensation ensures timely Cdc14 release and coordination of mitotic exit signaling with nucleolar segregation
Santos-Velázquez, Ana Isabel
Mitotic exit
SnoRNPs
Nucleolus
Condensin
FEAR
Cdc14
MEN
title_short Late rDNA condensation ensures timely Cdc14 release and coordination of mitotic exit signaling with nucleolar segregation
title_full Late rDNA condensation ensures timely Cdc14 release and coordination of mitotic exit signaling with nucleolar segregation
title_fullStr Late rDNA condensation ensures timely Cdc14 release and coordination of mitotic exit signaling with nucleolar segregation
title_full_unstemmed Late rDNA condensation ensures timely Cdc14 release and coordination of mitotic exit signaling with nucleolar segregation
title_sort Late rDNA condensation ensures timely Cdc14 release and coordination of mitotic exit signaling with nucleolar segregation
dc.creator.none.fl_str_mv Santos-Velázquez, Ana Isabel
García de Oya, Inés
Manzano-López, Javier
Monje-Casas, Fernando
author Santos-Velázquez, Ana Isabel
author_facet Santos-Velázquez, Ana Isabel
García de Oya, Inés
Manzano-López, Javier
Monje-Casas, Fernando
author_role author
author2 García de Oya, Inés
Manzano-López, Javier
Monje-Casas, Fernando
author2_role author
author
author
dc.contributor.none.fl_str_mv Junta de Andalucía
European Commission
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Mitotic exit
SnoRNPs
Nucleolus
Condensin
FEAR
Cdc14
MEN
topic Mitotic exit
SnoRNPs
Nucleolus
Condensin
FEAR
Cdc14
MEN
description The nucleolus plays a pivotal role in multiple key cellular processes. An illustrative example is the regulation of mitotic exit in Saccharomyces cerevisiae through the nucleolar sequestration of the Cdc14 phosphatase. The peculiar structure of the nucleolus, however, has also its drawbacks. The repetitive nature of the rDNA gives rise to cohesion-independent linkages whose resolution in budding yeast requires the Cdc14-dependent inhibition of rRNA transcription, which facilitates condensin accessibility to this locus. Thus, the rDNA condenses and segregates later than most other yeast genomic regions. Here, we show that defective function of a small nucleolar ribonucleoprotein particle (snoRNP) assembly factor facilitates condensin accessibility to the rDNA and induces nucleolar hyper-condensation. Interestingly, this increased compaction of the nucleolus interferes with the proper release of Cdc14 from this organelle. This observation provides an explanation for the delayed rDNA condensation in budding yeast, which is necessary to efficiently coordinate timely Cdc14 release and mitotic exit with nucleolar compaction and segregation.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/165562
url http://hdl.handle.net/10261/165562
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2013-43718-P
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2016-76642-P
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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