Replication Data for: BBB-on-a-chip with integrated micro-TEER for permeability evaluation of multi-functionalized gold nanorods against Alzheimer’s disease

Setup for Trans-endothelial electrical resistance (TEER) measurement in sterile conditions. Figure S2. Equivalent circuit and Nyquist plot of TEER from A) BBB-oC and B) control without EC and their fitting. Schema 2. Schematic representation of A) GNR-PEG-Ang2/D1 and B) Synthesis and functionalizati...

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Detalles Bibliográficos
Autores: Palma-Florez, Sujey, López-Canosa, Adrián, Moralez-Zavala, Francisco, Castaño, Oscar, Kogan, Marcelo J., Samitier Martí, Josep, Lagunas, Anna, Mir, Mònica
Tipo de recurso: conjunto de datos
Fecha de publicación:2019
País:España
Institución:Consorci de Serveis Universitaris de Catalunya (CSUC)
Repositorio:CORA.Repositori de Dades de Recerca
OAI Identifier:oai:dnet:cora.rdr____::9d2ade981e47f96a0c200299c7a01e3a
Acceso en línea:https://doi.org/10.34810/DATA771
Access Level:acceso abierto
Palabra clave:Medicine, Health and Life Sciences
Blood-Brain Barrier
Microphysiological Systems
Metal Nanoparticles
Gold
Alzheimer Disease
Electric Impedance
Endothelial Cells
Descripción
Sumario:Setup for Trans-endothelial electrical resistance (TEER) measurement in sterile conditions. Figure S2. Equivalent circuit and Nyquist plot of TEER from A) BBB-oC and B) control without EC and their fitting. Schema 2. Schematic representation of A) GNR-PEG-Ang2/D1 and B) Synthesis and functionalization process. Figure S3. Nanosystem characterization of GNR-CTAB, GNR-PEG and GNR-PEG-Ang2/D1. A) UV-vis-NIR absorbance spectrum B) Transversal and longitudinal hydrodynamic diameter (Dh), polydispersity index (Pdi) and zeta potential (pZ) C) TEM images obtained by transmission electron microscopy and D) histograms with their corresponding aspect ratio length/width distribution from 50 particles. Figure S4. Western blot of MCF7 and hCMEC/D3 cells lysate against LRP1 protein and actin. Figure S5. P values of GNR-PEG-Ang2/D1 exposed to endocytosis inhibition. Figure S6. TJs influence by D1 and Ang2 peptides by immunofluorescence and TEER. Fluorescence confocal images of TJs on Day 8 after incubation with D1 175,6nM or Ang2 69,2nM for 24 hours. Scale bar 50μm. Nyquist plot of TEER measurement (Ω.cm2) of BBB-oC before (Day 7) and next to peptide exposure for 24 hours.

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