Impedimetric label-free sensor for specific bacteria endotoxin detection by surface charge registration

An impedimetric sensor based on a three dimensional electrode array modified with concanavalin A (Con A) was used for label-free detection of bacterial endotoxin: lipopolysaccharide (LPS) from Escherichia coli. The transducer permits the detection of the surface charge changes due to interaction of...

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Autores: Brosel-Oliu, Sergi, Galyamin, Dmitry, Abramova, Natalia, Muñoz Pascual, Francisco Javier, Bratov, Andrey
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/164274
Acceso en línea:http://hdl.handle.net/10261/164274
Access Level:acceso abierto
Palabra clave:Impedimetric sensor
Surface charge
Interdigitated electrode array
Bacterial endotoxin
Concanavalin A
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spelling Impedimetric label-free sensor for specific bacteria endotoxin detection by surface charge registrationBrosel-Oliu, SergiGalyamin, DmitryAbramova, NataliaMuñoz Pascual, Francisco JavierBratov, AndreyImpedimetric sensorSurface chargeInterdigitated electrode arrayBacterial endotoxinConcanavalin AAn impedimetric sensor based on a three dimensional electrode array modified with concanavalin A (Con A) was used for label-free detection of bacterial endotoxin: lipopolysaccharide (LPS) from Escherichia coli. The transducer permits the detection of the surface charge changes due to interaction of immobilized Con A biorecognition element and LPS of E. coli in test solution. The deposition of Con A on the surface was carried out using the layer-by-layer method with polyethyleneimine (PEI) polycation as an initial layer. The sensor surface characterization by means of electrochemical impedance spectroscopy technique allowed registering variations in superficial resistance provoked by surface charge changes and is demonstrated as an effective method to monitor sensor parameters at each modification step as well as to follow Con A – LPS reaction. In order to prevent non-specific adsorption of LPS on PEI covered surface different blocking strategies were tested to achieve the specific response between Con A and LPS. Results obtained in this work clearly show that blocking with bovine serum albumin (BSA) is not sufficient to prevent non-specific interactions of PEI and to ensure the selective biorecognition of LPS by Con A. To achieve more efficient PEI blocking a new method was proposed based on consecutive deposition of Con A-glycogen-Con A layers. Sensors modified with PEI-(Con A-Gly)2-Con A multilayers are shown to be highly sensitive, selective and reproducible. Presented biosensor is able to detect bacterial LPS in a very short detection time (20 min) with 2 μg mL−1 limit of detection, which is much lower than reported for other biosensors with Con A.The authors acknowledge financial support from the Spanish Ministry of Economy and Competitiveness (project CTQ2014-54553-C3-1-R and S.B.O. fellowship of FPI-MICINN program BES-2015-071250) co-funded by the European Regional Development Fund (Feder) and from the Government of Russian Federation (Grant 074-U01).Peer reviewedElsevierMinisterio de Economía, Industria y Competitividad (España)European CommissionMinisterio de Economía y Competitividad (España)Ministerio de Ciencia e Innovación (España)Russian GovernmentConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201820182017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/164274reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2014-54553-C3-1-Rhttp://doi.org/10.1016/j.electacta.2017.05.060Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1642742026-05-22T06:33:51Z
dc.title.none.fl_str_mv Impedimetric label-free sensor for specific bacteria endotoxin detection by surface charge registration
title Impedimetric label-free sensor for specific bacteria endotoxin detection by surface charge registration
spellingShingle Impedimetric label-free sensor for specific bacteria endotoxin detection by surface charge registration
Brosel-Oliu, Sergi
Impedimetric sensor
Surface charge
Interdigitated electrode array
Bacterial endotoxin
Concanavalin A
title_short Impedimetric label-free sensor for specific bacteria endotoxin detection by surface charge registration
title_full Impedimetric label-free sensor for specific bacteria endotoxin detection by surface charge registration
title_fullStr Impedimetric label-free sensor for specific bacteria endotoxin detection by surface charge registration
title_full_unstemmed Impedimetric label-free sensor for specific bacteria endotoxin detection by surface charge registration
title_sort Impedimetric label-free sensor for specific bacteria endotoxin detection by surface charge registration
dc.creator.none.fl_str_mv Brosel-Oliu, Sergi
Galyamin, Dmitry
Abramova, Natalia
Muñoz Pascual, Francisco Javier
Bratov, Andrey
author Brosel-Oliu, Sergi
author_facet Brosel-Oliu, Sergi
Galyamin, Dmitry
Abramova, Natalia
Muñoz Pascual, Francisco Javier
Bratov, Andrey
author_role author
author2 Galyamin, Dmitry
Abramova, Natalia
Muñoz Pascual, Francisco Javier
Bratov, Andrey
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía, Industria y Competitividad (España)
European Commission
Ministerio de Economía y Competitividad (España)
Ministerio de Ciencia e Innovación (España)
Russian Government
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Impedimetric sensor
Surface charge
Interdigitated electrode array
Bacterial endotoxin
Concanavalin A
topic Impedimetric sensor
Surface charge
Interdigitated electrode array
Bacterial endotoxin
Concanavalin A
description An impedimetric sensor based on a three dimensional electrode array modified with concanavalin A (Con A) was used for label-free detection of bacterial endotoxin: lipopolysaccharide (LPS) from Escherichia coli. The transducer permits the detection of the surface charge changes due to interaction of immobilized Con A biorecognition element and LPS of E. coli in test solution. The deposition of Con A on the surface was carried out using the layer-by-layer method with polyethyleneimine (PEI) polycation as an initial layer. The sensor surface characterization by means of electrochemical impedance spectroscopy technique allowed registering variations in superficial resistance provoked by surface charge changes and is demonstrated as an effective method to monitor sensor parameters at each modification step as well as to follow Con A – LPS reaction. In order to prevent non-specific adsorption of LPS on PEI covered surface different blocking strategies were tested to achieve the specific response between Con A and LPS. Results obtained in this work clearly show that blocking with bovine serum albumin (BSA) is not sufficient to prevent non-specific interactions of PEI and to ensure the selective biorecognition of LPS by Con A. To achieve more efficient PEI blocking a new method was proposed based on consecutive deposition of Con A-glycogen-Con A layers. Sensors modified with PEI-(Con A-Gly)2-Con A multilayers are shown to be highly sensitive, selective and reproducible. Presented biosensor is able to detect bacterial LPS in a very short detection time (20 min) with 2 μg mL−1 limit of detection, which is much lower than reported for other biosensors with Con A.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/164274
url http://hdl.handle.net/10261/164274
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2014-54553-C3-1-R
http://doi.org/10.1016/j.electacta.2017.05.060
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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