Efficient Procedure for Induction Somatic Embryogenesis in Holm Oak: Roles of Explant Type, Auxin Type, and Exposure Duration to Auxin

Holm oak is the dominant tree species in the Mediterranean climate. Currently, worrisome degradation of its ecosystems has been observed, produced, among other factors, by changes in land use, extreme weather events, forest fires, climate change, and especially the increasingly frequent episodes of...

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Detalles Bibliográficos
Autores: Martínez-Santiago, María Teresa, Corredoira, Elena
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/296834
Acceso en línea:http://hdl.handle.net/10261/296834
https://api.elsevier.com/content/abstract/scopus_id/85149026913
Access Level:acceso abierto
Palabra clave:1-Naphthaleneacetic acid
Auxin
Indole-3-acetic acid
Indole-3-butyric acid
Leaves
Node explants
Quercus ilex
Shoot apex
Somatic embryogenesis induction
http://metadata.un.org/sdg/13
Take urgent action to combat climate change and its impacts
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spelling Efficient Procedure for Induction Somatic Embryogenesis in Holm Oak: Roles of Explant Type, Auxin Type, and Exposure Duration to AuxinMartínez-Santiago, María TeresaCorredoira, Elena1-Naphthaleneacetic acidAuxinIndole-3-acetic acidIndole-3-butyric acidLeavesNode explantsQuercus ilexShoot apexSomatic embryogenesis inductionhttp://metadata.un.org/sdg/13Take urgent action to combat climate change and its impactsHolm oak is the dominant tree species in the Mediterranean climate. Currently, worrisome degradation of its ecosystems has been observed, produced, among other factors, by changes in land use, extreme weather events, forest fires, climate change, and especially the increasingly frequent episodes of high tree mortality caused by “oak decline”, which has brought with it a social concern that transcends the productive interest. Breeding and conservation programs for this species are necessary to ensure the prevalence of these ecosystems for future generations. Biotechnological tools such as somatic embryogenesis (SE) have great potential value for tree improvement and have been shown to be highly efficient in the propagation and conservation of woody species. One challenge to this approach is that SE induction in holm oak has not yet been optimized. Here, we present a new reproducible procedure to induce SE in holm oak; we evaluated the responsiveness of different initial explants exposed to different types, concentrations, and durations of auxin. SE rates were significantly improved (37%) by culturing nodal segments for two weeks in induction medium. In addition, a significant auxin–genotype interaction was observed.This research was funded in part by MINECO (Spain), MICINN (Spain), and MITECO-TRAGSA (Spain) through the projects AGL2016-76143-C4-4-R and PID2020-112627RB-C33 and through the contract TSA0068372, respectively.Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Economía y Competitividad (España)Ministerio de Ciencia e Innovación (España)Ministerio de Transición Ecológica (España)TRAGSA Empresa de Transformación AgrariaMartínez, María Teresa [0000-0002-7416-875X]Corredoira, Elena [0000-0003-2839-8244]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/296834https://api.elsevier.com/content/abstract/scopus_id/85149026913reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO//AGL2016-76143-C4-4-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-112627RB-C33Forestshttps://doi.org/10.3390/f14020430Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2968342026-05-22T06:33:51Z
dc.title.none.fl_str_mv Efficient Procedure for Induction Somatic Embryogenesis in Holm Oak: Roles of Explant Type, Auxin Type, and Exposure Duration to Auxin
title Efficient Procedure for Induction Somatic Embryogenesis in Holm Oak: Roles of Explant Type, Auxin Type, and Exposure Duration to Auxin
spellingShingle Efficient Procedure for Induction Somatic Embryogenesis in Holm Oak: Roles of Explant Type, Auxin Type, and Exposure Duration to Auxin
Martínez-Santiago, María Teresa
1-Naphthaleneacetic acid
Auxin
Indole-3-acetic acid
Indole-3-butyric acid
Leaves
Node explants
Quercus ilex
Shoot apex
Somatic embryogenesis induction
http://metadata.un.org/sdg/13
Take urgent action to combat climate change and its impacts
title_short Efficient Procedure for Induction Somatic Embryogenesis in Holm Oak: Roles of Explant Type, Auxin Type, and Exposure Duration to Auxin
title_full Efficient Procedure for Induction Somatic Embryogenesis in Holm Oak: Roles of Explant Type, Auxin Type, and Exposure Duration to Auxin
title_fullStr Efficient Procedure for Induction Somatic Embryogenesis in Holm Oak: Roles of Explant Type, Auxin Type, and Exposure Duration to Auxin
title_full_unstemmed Efficient Procedure for Induction Somatic Embryogenesis in Holm Oak: Roles of Explant Type, Auxin Type, and Exposure Duration to Auxin
title_sort Efficient Procedure for Induction Somatic Embryogenesis in Holm Oak: Roles of Explant Type, Auxin Type, and Exposure Duration to Auxin
dc.creator.none.fl_str_mv Martínez-Santiago, María Teresa
Corredoira, Elena
author Martínez-Santiago, María Teresa
author_facet Martínez-Santiago, María Teresa
Corredoira, Elena
author_role author
author2 Corredoira, Elena
author2_role author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Ministerio de Ciencia e Innovación (España)
Ministerio de Transición Ecológica (España)
TRAGSA Empresa de Transformación Agraria
Martínez, María Teresa [0000-0002-7416-875X]
Corredoira, Elena [0000-0003-2839-8244]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv 1-Naphthaleneacetic acid
Auxin
Indole-3-acetic acid
Indole-3-butyric acid
Leaves
Node explants
Quercus ilex
Shoot apex
Somatic embryogenesis induction
http://metadata.un.org/sdg/13
Take urgent action to combat climate change and its impacts
topic 1-Naphthaleneacetic acid
Auxin
Indole-3-acetic acid
Indole-3-butyric acid
Leaves
Node explants
Quercus ilex
Shoot apex
Somatic embryogenesis induction
http://metadata.un.org/sdg/13
Take urgent action to combat climate change and its impacts
description Holm oak is the dominant tree species in the Mediterranean climate. Currently, worrisome degradation of its ecosystems has been observed, produced, among other factors, by changes in land use, extreme weather events, forest fires, climate change, and especially the increasingly frequent episodes of high tree mortality caused by “oak decline”, which has brought with it a social concern that transcends the productive interest. Breeding and conservation programs for this species are necessary to ensure the prevalence of these ecosystems for future generations. Biotechnological tools such as somatic embryogenesis (SE) have great potential value for tree improvement and have been shown to be highly efficient in the propagation and conservation of woody species. One challenge to this approach is that SE induction in holm oak has not yet been optimized. Here, we present a new reproducible procedure to induce SE in holm oak; we evaluated the responsiveness of different initial explants exposed to different types, concentrations, and durations of auxin. SE rates were significantly improved (37%) by culturing nodal segments for two weeks in induction medium. In addition, a significant auxin–genotype interaction was observed.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/296834
https://api.elsevier.com/content/abstract/scopus_id/85149026913
url http://hdl.handle.net/10261/296834
https://api.elsevier.com/content/abstract/scopus_id/85149026913
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO//AGL2016-76143-C4-4-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-112627RB-C33
Forests
https://doi.org/10.3390/f14020430
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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