Hyaluronic Acid Combined with Serum Rich in Growth Factors in Corneal Epithelial Defects

The aim of this study is to assess if an adhesive biopolymer, sodium hyaluronate (NaHA), has synergistic effects with s-PRGF (a serum derived from plasma rich in growth factors and a blood derivative that has already shown efficacy in corneal epithelial wound healing), to reduce time of healing or p...

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Authors: Suárez Barrio, Carlota, Etxebarria, Jaime, Hernáez Moya, Raquel, Del Val Alonso, Marina, Rodríguez Astigarraga, Maddalen, Urkaregi Etxepare, Arantza, Freire Vallejo, Vanesa, Morales González, María Celia, Durán de la Colina, Juan Antonio, Vicario, Marta, Molina, Irene, Herrero Vanrell, Rocío, Andollo Victoriano, María Noelia
Format: article
Publication Date:2019
Country:España
Institution:Universidad del País Vasco
Repository:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/32703
Online Access:http://hdl.handle.net/10810/32703
Access Level:Open access
Keyword:cornea regeneration
corneal epithelial defect
hyaluronic acid (NaHA)
plasma rich plasma (PRP)
serum derived from plasma rich in growth factors (s-PRGF)
serum eye drops
wound healing
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spelling Hyaluronic Acid Combined with Serum Rich in Growth Factors in Corneal Epithelial DefectsSuárez Barrio, CarlotaEtxebarria, JaimeHernáez Moya, RaquelDel Val Alonso, MarinaRodríguez Astigarraga, MaddalenUrkaregi Etxepare, ArantzaFreire Vallejo, VanesaMorales González, María CeliaDurán de la Colina, Juan AntonioVicario, MartaMolina, IreneHerrero Vanrell, RocíoAndollo Victoriano, María Noeliacornea regenerationcorneal epithelial defecthyaluronic acid (NaHA)plasma rich plasma (PRP)serum derived from plasma rich in growth factors (s-PRGF)serum eye dropswound healingThe aim of this study is to assess if an adhesive biopolymer, sodium hyaluronate (NaHA), has synergistic effects with s-PRGF (a serum derived from plasma rich in growth factors and a blood derivative that has already shown efficacy in corneal epithelial wound healing), to reduce time of healing or posology. In vitro proliferation and migration studies, both in human corneal epithelial (HCE) cells and in rabbit primary corneal epithelial (RPCE) cultures, were carried out. In addition, we performed studies of corneal wound healing in vivo in rabbits treated with s-PRGF, NaHA, or the combination of both. We performed immunohistochemistry techniques (CK3, CK15, Ki67, SS4 integrin, ZO-1, alpha-SMA) in rabbit corneas 7 and 30 days after a surgically induced epithelial defect. In vitro results show that the combination of NaHA and s-PRGF offers the worst proliferation rates in both HCE and RPCE cells. Addition of NaHA to s-PRGF diminishes the re-epithelializing capability of s-PRGF. In vivo, all treatments, given twice a day, showed equivalent efficacy in corneal epithelial healing. We conclude that the combined use of s-PRGF and HaNA as an adhesive biopolymer does not improve the efficacy of s-PRGF alone in the wound healing of corneal epithelial defects.Research study supported by grants from the University of the Basque Country UPV/EHU-Instituto Clinico Quirurgico de Oftalmologia ICQO (US16/23), and from the Basque Foundation for Health Research and Innovation BIOEF (BIO14/TP/002).MDPI201920192019info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/32703reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoIngléshttps://www.mdpi.com/1422-0067/20/7/1655info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).Atribución 3.0 Españaoai:addi.ehu.eus:10810/327032026-06-18T09:23:17Z
dc.title.none.fl_str_mv Hyaluronic Acid Combined with Serum Rich in Growth Factors in Corneal Epithelial Defects
title Hyaluronic Acid Combined with Serum Rich in Growth Factors in Corneal Epithelial Defects
spellingShingle Hyaluronic Acid Combined with Serum Rich in Growth Factors in Corneal Epithelial Defects
Suárez Barrio, Carlota
cornea regeneration
corneal epithelial defect
hyaluronic acid (NaHA)
plasma rich plasma (PRP)
serum derived from plasma rich in growth factors (s-PRGF)
serum eye drops
wound healing
title_short Hyaluronic Acid Combined with Serum Rich in Growth Factors in Corneal Epithelial Defects
title_full Hyaluronic Acid Combined with Serum Rich in Growth Factors in Corneal Epithelial Defects
title_fullStr Hyaluronic Acid Combined with Serum Rich in Growth Factors in Corneal Epithelial Defects
title_full_unstemmed Hyaluronic Acid Combined with Serum Rich in Growth Factors in Corneal Epithelial Defects
title_sort Hyaluronic Acid Combined with Serum Rich in Growth Factors in Corneal Epithelial Defects
dc.creator.none.fl_str_mv Suárez Barrio, Carlota
Etxebarria, Jaime
Hernáez Moya, Raquel
Del Val Alonso, Marina
Rodríguez Astigarraga, Maddalen
Urkaregi Etxepare, Arantza
Freire Vallejo, Vanesa
Morales González, María Celia
Durán de la Colina, Juan Antonio
Vicario, Marta
Molina, Irene
Herrero Vanrell, Rocío
Andollo Victoriano, María Noelia
author Suárez Barrio, Carlota
author_facet Suárez Barrio, Carlota
Etxebarria, Jaime
Hernáez Moya, Raquel
Del Val Alonso, Marina
Rodríguez Astigarraga, Maddalen
Urkaregi Etxepare, Arantza
Freire Vallejo, Vanesa
Morales González, María Celia
Durán de la Colina, Juan Antonio
Vicario, Marta
Molina, Irene
Herrero Vanrell, Rocío
Andollo Victoriano, María Noelia
author_role author
author2 Etxebarria, Jaime
Hernáez Moya, Raquel
Del Val Alonso, Marina
Rodríguez Astigarraga, Maddalen
Urkaregi Etxepare, Arantza
Freire Vallejo, Vanesa
Morales González, María Celia
Durán de la Colina, Juan Antonio
Vicario, Marta
Molina, Irene
Herrero Vanrell, Rocío
Andollo Victoriano, María Noelia
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv cornea regeneration
corneal epithelial defect
hyaluronic acid (NaHA)
plasma rich plasma (PRP)
serum derived from plasma rich in growth factors (s-PRGF)
serum eye drops
wound healing
topic cornea regeneration
corneal epithelial defect
hyaluronic acid (NaHA)
plasma rich plasma (PRP)
serum derived from plasma rich in growth factors (s-PRGF)
serum eye drops
wound healing
description The aim of this study is to assess if an adhesive biopolymer, sodium hyaluronate (NaHA), has synergistic effects with s-PRGF (a serum derived from plasma rich in growth factors and a blood derivative that has already shown efficacy in corneal epithelial wound healing), to reduce time of healing or posology. In vitro proliferation and migration studies, both in human corneal epithelial (HCE) cells and in rabbit primary corneal epithelial (RPCE) cultures, were carried out. In addition, we performed studies of corneal wound healing in vivo in rabbits treated with s-PRGF, NaHA, or the combination of both. We performed immunohistochemistry techniques (CK3, CK15, Ki67, SS4 integrin, ZO-1, alpha-SMA) in rabbit corneas 7 and 30 days after a surgically induced epithelial defect. In vitro results show that the combination of NaHA and s-PRGF offers the worst proliferation rates in both HCE and RPCE cells. Addition of NaHA to s-PRGF diminishes the re-epithelializing capability of s-PRGF. In vivo, all treatments, given twice a day, showed equivalent efficacy in corneal epithelial healing. We conclude that the combined use of s-PRGF and HaNA as an adhesive biopolymer does not improve the efficacy of s-PRGF alone in the wound healing of corneal epithelial defects.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/32703
url http://hdl.handle.net/10810/32703
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://www.mdpi.com/1422-0067/20/7/1655
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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