Detection of growth hormone releasing peptides in serum by a Competitive receptor binding assay

The use of small peptides for medical purposes has gained great importance in the last decade. Among these molecules are the growth hormone releasing peptides (GHRPs). These substances are highly sought by cheating athletes because of their ergogenic effects. Thus, GHRPs are listed in the World Anti...

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Autores: Ferro, P., Krotov, G., Zvereva, I., Pérez Mañá, Clara, Mateus Rodriguez, Julian Andrés, Segura, Joan
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Universitat Pompeu Fabra
Repositorio:Repositorio Digital de la UPF
OAI Identifier:oai:repositori.upf.edu:10230/46039
Acceso en línea:http://hdl.handle.net/10230/46039
http://dx.doi.org/10.4172/2157-7064.1000351
Access Level:acceso abierto
Palabra clave:Growth hormone secretagogue
Ghrelin
Radiocompetition assay
Doping substances
Doping abuse
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spelling Detection of growth hormone releasing peptides in serum by a Competitive receptor binding assayFerro, P.Krotov, G.Zvereva, I.Pérez Mañá, ClaraMateus Rodriguez, Julian AndrésSegura, JoanGrowth hormone secretagogueGhrelinRadiocompetition assayDoping substancesDoping abuseThe use of small peptides for medical purposes has gained great importance in the last decade. Among these molecules are the growth hormone releasing peptides (GHRPs). These substances are highly sought by cheating athletes because of their ergogenic effects. Thus, GHRPs are listed in the World Antidoping Agency Prohibited List and several methods have been developed for their detection in urine samples. Serum is another useful matrix frequently used for anti-doping control. An experimental adaptation of a competitive methodology for GHRPs wide-group detection, previously developed for urine, is reported here using a serum matrix instead. The modifications have been mainly addressed the need to remove endogenous ghrelin (always present in serum) to avoid interference in the competitive binding assay. The modified test was first evaluated using serum spiked with different pure GHRPs (i.e., GHRP-2, GHRP-6 and Hexarelin), then using serum samples from pilot studies in which healthy subjects were treated intravenously with GHRP-2 and intranasally with GHRP-2 and GHRP-6. The results showed the reliability of the modified methodology for the detection of GHRPs in a serum matrixElsevier202020202017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/46039http://dx.doi.org/10.4172/2157-7064.1000351reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésJournal of Chromatography and Separation Techniques. 2017;8:351Copyright: © 2017 Ferro P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/460392026-06-12T07:21:37Z
dc.title.none.fl_str_mv Detection of growth hormone releasing peptides in serum by a Competitive receptor binding assay
title Detection of growth hormone releasing peptides in serum by a Competitive receptor binding assay
spellingShingle Detection of growth hormone releasing peptides in serum by a Competitive receptor binding assay
Ferro, P.
Growth hormone secretagogue
Ghrelin
Radiocompetition assay
Doping substances
Doping abuse
title_short Detection of growth hormone releasing peptides in serum by a Competitive receptor binding assay
title_full Detection of growth hormone releasing peptides in serum by a Competitive receptor binding assay
title_fullStr Detection of growth hormone releasing peptides in serum by a Competitive receptor binding assay
title_full_unstemmed Detection of growth hormone releasing peptides in serum by a Competitive receptor binding assay
title_sort Detection of growth hormone releasing peptides in serum by a Competitive receptor binding assay
dc.creator.none.fl_str_mv Ferro, P.
Krotov, G.
Zvereva, I.
Pérez Mañá, Clara
Mateus Rodriguez, Julian Andrés
Segura, Joan
author Ferro, P.
author_facet Ferro, P.
Krotov, G.
Zvereva, I.
Pérez Mañá, Clara
Mateus Rodriguez, Julian Andrés
Segura, Joan
author_role author
author2 Krotov, G.
Zvereva, I.
Pérez Mañá, Clara
Mateus Rodriguez, Julian Andrés
Segura, Joan
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Growth hormone secretagogue
Ghrelin
Radiocompetition assay
Doping substances
Doping abuse
topic Growth hormone secretagogue
Ghrelin
Radiocompetition assay
Doping substances
Doping abuse
description The use of small peptides for medical purposes has gained great importance in the last decade. Among these molecules are the growth hormone releasing peptides (GHRPs). These substances are highly sought by cheating athletes because of their ergogenic effects. Thus, GHRPs are listed in the World Antidoping Agency Prohibited List and several methods have been developed for their detection in urine samples. Serum is another useful matrix frequently used for anti-doping control. An experimental adaptation of a competitive methodology for GHRPs wide-group detection, previously developed for urine, is reported here using a serum matrix instead. The modifications have been mainly addressed the need to remove endogenous ghrelin (always present in serum) to avoid interference in the competitive binding assay. The modified test was first evaluated using serum spiked with different pure GHRPs (i.e., GHRP-2, GHRP-6 and Hexarelin), then using serum samples from pilot studies in which healthy subjects were treated intravenously with GHRP-2 and intranasally with GHRP-2 and GHRP-6. The results showed the reliability of the modified methodology for the detection of GHRPs in a serum matrix
publishDate 2017
dc.date.none.fl_str_mv 2017
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/46039
http://dx.doi.org/10.4172/2157-7064.1000351
url http://hdl.handle.net/10230/46039
http://dx.doi.org/10.4172/2157-7064.1000351
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Journal of Chromatography and Separation Techniques. 2017;8:351
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositorio Digital de la UPF
instname:Universitat Pompeu Fabra
instname_str Universitat Pompeu Fabra
reponame_str Repositorio Digital de la UPF
collection Repositorio Digital de la UPF
repository.name.fl_str_mv
repository.mail.fl_str_mv
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